Yıl 2019, Cilt 20 , Sayı 1, Sayfalar 63 - 70 2019-04-15

DETERMINATION OF GROWTH KINETICS AND BIOCHEMICAL COMPOSITION OF Nitzschia palea (Kützing) W. Smith ISOLATED FROM FRESHWATER SOURCES IN TURKEY

Dilek YALÇIN DUYGU [1]


Bu çalışma, daha ileri çalışmaların önünü açmak amacıyla yığın kültür koşullarında, Nitzschia palea (Kützing) W. Smith diatom türünün biyokimyasal tayini ve büyüme dinamikleri hakkında ayrıntılı bilgi vermek amacıyla yapılmıştır. Çalışma materyali, Ankara, Türkiye'den toplanan tatlı su örneğinden izole edilmiştir. Diatom kültürü Allen besi ortamında 168 saat boyunca yetiştirilmiş ve büyüme dinamikleri hücre yoğunluğu ile kuru ağırlık analizleriyle belirlenmiştir. Spesifik büyüme hızı, kültürün ikilenme süresi ve biyokimyasal bileşimleri de incelenmiştir. Nitzschia palea’nın moleküler karakterizasyonu Fourier Transform Infrared Spektroskopisi kullanılarak gerçekleştirilmiştir. Ekimi takip eden 168. saatte, Allen besi kültür ortamında kültürlerin hücre yoğunluğu 2,0x106±1,0x105 hücre/mL ve kuru biyokütlesi 0,212±0,041 (g L-1) olarak tespit edilmiştir. Nitzschia palea’nın spesifik büyüme oranı 96. saatte (0,010 h-1) ve ikilenme süresi 68 h-1 olarak hesaplanmıştır. Nitzschia palea’nın protein miktarı (%41,21), karbonhidrat miktarı (%21,74), lipit miktarı (%16,84) ve kül miktarı (%19,88) olarak belirlenmiştir. Bu sonuçlar, N. palea'nın farklı endüstri alanlarında özellikle biyodizel üretimi için kullanılabileceğini göstermiştir.

This study was performed in order to bring out a detailed information on growth dynamics and biochemical determination of the diatom species Nitzschia palea (Kützing) W. Smith under batch culture conditions in order to pave the way for further studies. The study material was isolated from a fresh water sample collected from Ankara, Turkey. The diatoms were cultured in Allen medium for 168 hours and the growth dynamics were determined by cell density and dry weight analyses. Specific growth rate, duplication time of the culture and biochemical compositions were also investigated. Molecular characterization of the N. palea strain was performed by applying Fourier Transform Infrared Spectroscopy. The cell density and the dry biomass of the culture at the end of the 168 hours incubation period was determined as 2.0x106±1.0x105cells/mL and 0.212±0.041 g L-1, respectively. The algal specific growth rate was found as 0.010 h-1 at 96-h and the doubling time was calculated as 68 h-1. The protein content was measured as 41.21%, carbohydrate content as 21.74%, lipid content as 16.84% and ash content as 19.88%. These results indicated that N. palea may be used in different fields of industries, especially in biodiesel production.

batch culture, lipid, carbohydrate, protein, ash, Nitzschia palea, ash, FTIR
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Birincil Dil en
Konular Deniz ve Tatlı Su Biyolojisi
Bölüm Araştırma Makalesi/Research Article
Yazarlar

Orcid: 0000-0003-2127-8186
Yazar: Dilek YALÇIN DUYGU (Sorumlu Yazar)
Kurum: GAZI UNIVERSITY, GAZİ FACULTY OF EDUCATION
Ülke: Turkey


Tarihler

Başvuru Tarihi : 17 Aralık 2018
Kabul Tarihi : 22 Mart 2019
Yayımlanma Tarihi : 15 Nisan 2019

Bibtex @araştırma makalesi { trkjnat498426, journal = {Trakya University Journal of Natural Sciences}, issn = {2147-0294}, eissn = {2528-9691}, address = {}, publisher = {Trakya Üniversitesi}, year = {2019}, volume = {20}, pages = {63 - 70}, doi = {10.23902/trkjnat.498426}, title = {DETERMINATION OF GROWTH KINETICS AND BIOCHEMICAL COMPOSITION OF Nitzschia palea (Kützing) W. Smith ISOLATED FROM FRESHWATER SOURCES IN TURKEY}, key = {cite}, author = {YALÇIN DUYGU, Dilek} }
APA YALÇIN DUYGU, D . (2019). DETERMINATION OF GROWTH KINETICS AND BIOCHEMICAL COMPOSITION OF Nitzschia palea (Kützing) W. Smith ISOLATED FROM FRESHWATER SOURCES IN TURKEY. Trakya University Journal of Natural Sciences , 20 (1) , 63-70 . DOI: 10.23902/trkjnat.498426
MLA YALÇIN DUYGU, D . "DETERMINATION OF GROWTH KINETICS AND BIOCHEMICAL COMPOSITION OF Nitzschia palea (Kützing) W. Smith ISOLATED FROM FRESHWATER SOURCES IN TURKEY". Trakya University Journal of Natural Sciences 20 (2019 ): 63-70 <https://dergipark.org.tr/tr/pub/trkjnat/issue/44590/498426>
Chicago YALÇIN DUYGU, D . "DETERMINATION OF GROWTH KINETICS AND BIOCHEMICAL COMPOSITION OF Nitzschia palea (Kützing) W. Smith ISOLATED FROM FRESHWATER SOURCES IN TURKEY". Trakya University Journal of Natural Sciences 20 (2019 ): 63-70
RIS TY - JOUR T1 - DETERMINATION OF GROWTH KINETICS AND BIOCHEMICAL COMPOSITION OF Nitzschia palea (Kützing) W. Smith ISOLATED FROM FRESHWATER SOURCES IN TURKEY AU - Dilek YALÇIN DUYGU Y1 - 2019 PY - 2019 N1 - doi: 10.23902/trkjnat.498426 DO - 10.23902/trkjnat.498426 T2 - Trakya University Journal of Natural Sciences JF - Journal JO - JOR SP - 63 EP - 70 VL - 20 IS - 1 SN - 2147-0294-2528-9691 M3 - doi: 10.23902/trkjnat.498426 UR - https://doi.org/10.23902/trkjnat.498426 Y2 - 2019 ER -
EndNote %0 Trakya University Journal of Natural Sciences DETERMINATION OF GROWTH KINETICS AND BIOCHEMICAL COMPOSITION OF Nitzschia palea (Kützing) W. Smith ISOLATED FROM FRESHWATER SOURCES IN TURKEY %A Dilek YALÇIN DUYGU %T DETERMINATION OF GROWTH KINETICS AND BIOCHEMICAL COMPOSITION OF Nitzschia palea (Kützing) W. Smith ISOLATED FROM FRESHWATER SOURCES IN TURKEY %D 2019 %J Trakya University Journal of Natural Sciences %P 2147-0294-2528-9691 %V 20 %N 1 %R doi: 10.23902/trkjnat.498426 %U 10.23902/trkjnat.498426
ISNAD YALÇIN DUYGU, Dilek . "DETERMINATION OF GROWTH KINETICS AND BIOCHEMICAL COMPOSITION OF Nitzschia palea (Kützing) W. Smith ISOLATED FROM FRESHWATER SOURCES IN TURKEY". Trakya University Journal of Natural Sciences 20 / 1 (Nisan 2019): 63-70 . https://doi.org/10.23902/trkjnat.498426
AMA YALÇIN DUYGU D . DETERMINATION OF GROWTH KINETICS AND BIOCHEMICAL COMPOSITION OF Nitzschia palea (Kützing) W. Smith ISOLATED FROM FRESHWATER SOURCES IN TURKEY. Trakya Univ J Nat Sci. 2019; 20(1): 63-70.
Vancouver YALÇIN DUYGU D . DETERMINATION OF GROWTH KINETICS AND BIOCHEMICAL COMPOSITION OF Nitzschia palea (Kützing) W. Smith ISOLATED FROM FRESHWATER SOURCES IN TURKEY. Trakya University Journal of Natural Sciences. 2019; 20(1): 70-63.