Boldenon Uygulamasının Egzersiz Yaptırılan Sıçanlarda Bazı Organ Hasarı Belirteçlerine Etkileri

Öz

Bu çalışmanın amacı boldenon uygulamasının egzersiz yaptırılan sıçanlarda iskelet kası, karaciğer ve kalp organlarına etkilerini incelemektir. Ratlar, kontrol (C), egzersiz (E), boldenon (B) ve boldenon + egzersiz (BE) olmak üzere 4 gruba ayrıldı. C ve E gruplarında 6, diğer gruplarda ise 7’şer sıçan vardı. E ve BE gruplarındaki sıçanlara 8 hafta boyunca 1.5km/saat hızda olacak şekilde haftada 5 gün 45 dakikalık koşu egzersizi yaptırıldı. Buna ek olarak B ve BE grubundaki sıçanlara haftada 10mg/kg dozda Boldenon uygulaması yapıldı. Verilerin istatistiki değerlendirilmesinde SPSS 22 paket programı kullanılarak sonuçlar ort±SS olarak verildi. Verilerin gruplar arası karşılaştırılmalarında ANOVA ve Duncan testi uygulandı. 8 Haftalık çalışmanın sonunda sıçanlardan alınan kan örneklerinde Yapılan istatistiki inceleme sonucunda Aspartat Aminotransferaz (AST) ve Kreatin fosfokinaz (CK-MB) değerlerinin B ve BE gruplarında C ve E gruplarına göre anlamlı ölçüde yüksek çıktığı görülmüştür (p<0,05). Alanin Aminotransferaz (ALT) ve Alkalen Fosfataz (ALP) değerlerinin ise diğer gruplarla kıyaslandığında E grubunda anlamlı ölçüde düşük çıktığı gözlemlenmiştir (p<0,05), her iki parametrede de B ve BE grubu ortalama değerlerinin C ve E grubu ortalama değerlerinden daha yüksek çıktığı görülmüştür. Laktat dehidrogenaz (LDH) değerleri ise E, B ve BE gruplarında C grubuna göre anlamlı ölçüde yüksek çıkmıştır (p<0,05). Bulgulardan elde edilen sonuçla; Anabolik-androjenik steroid (AAS) kullanımının karaciğer enzim seviyelerini (ALT, AST, ALP, LDH) ve kalp kası hasarının belirteci olan CK-MB seviyelerini artırdığı gözlemlenmiştir. Bu bulgularla AAS kullanımının kalp ve karaciğer üzerine olumsuz etkileri olduğu söylenebilir. Ayrıca egzersiz yapmanın AAS’lerin bu olumsuz yan etkilerinin miktarını azaltmadığı gibi, BE grubunun ortalama değerlerinin tüm parametrelerde diğer gruplardan daha yüksek çıkması, egzersizin bu yan etkileri daha da artırıyor olabileceğini gösterebilmektedir.

Anahtar Kelimeler

• Anabolik androjenik steroidler
• Boldenon
• Egzersiz
• İskelet kası
• Kalp

Effect of Boldenone Administration on Some Organ Damage Markers in Trained Rats

Öz

The aim of this study was to examine the effects of Boldenone administration on skeletal muscle, liver and heart organs in exercised rats. Rats were divided into 4 groups as Control(C), Exercise (E), Boldenone(B) and Boldenone + Exercise (BE). There are 6 rats in the C and E groups and 7 rats in the other groups. The rats in groups E and BE were given a 45-minute treadmill exercise 5 days a week at a speed of 1.5km/hour for 8 weeks. In the statistical evaluation of the data, the results were given as mean±SD using the SPSS 22 package program. ANOVA and Duncan tests were used to compare the data between groups. At the end of the 8-week study, blood samples taken from rats were found to be significantly higher in AST and CK-MB values in B and BE groups than in C and E groups as a result of the statistical analysis (p0,05), it was observed that the mean values of the B and BE groups were higher than the mean values of the C and E groups in both parameters. LDH values were significantly higher in E, B and BE groups compared to C group (p<0.05). With the result obtained from the findings; It has been observed that the use of AAS increases liver enzyme levels (ALT, AST, ALP, LDH) and CK-MB levels, which is a marker of heart muscle damage. With these findings, it can be said that the use of Anabolic Androgenic Steroids has negative effects on the heart and liver. In addition, the fact that the mean values of the BE group were higher than the other groups in all parameters, as exercise did not reduce the amount of these negative side effects of AASs, indicates that exercise may increase these side effects even more.

Anahtar Kelimeler

• Skeletal muscle.
• Boldenone
• Anabolic androgenic steroids

Kaynakça

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