HISTOLOGICAL EVALUATION OF (4S)-2-(4-HYDROXY-3-METHOXYPHENYL) THIAZOLIDINE-4-CARBOXYLIC ACID ON ZEBRAFISH (Danio rerio) GILLS
Öz
Investigation
the histopathological effects of (4s)-2-(4-hydroxy-3-methoxyphenyl)
thiazolidine-4-carboxylic acid on gill tissue of zebrafish were aimed.
(4S)-2-(4-hydroxy-3-methoxyphenyl)
thiazolidine-4-carboxylic acid is new synthesized substance which obtained from
cysteine and valine. Because of thiazolidine derivates have important
biological responses scientist work intensively on these compounds recent
years. It is obvious that thiazolidine contained compounds will be used in
future in the pharmaceutical industry to treat important diseases.
After one-week
adaptation period zebrafish divided into four group (n=10) as one control and 3
experimental groups (0.2 mM, 0.4mM 0.6mM). Gills were dissected after 5 day of
the exposure. Tissues were fixed with 10% neutral buffered formalin and dehydration
were carried out in an ascending series of ethanol. Tissues were cleared in
xylene, embedded in paraffin wax and cut into 5 µm sections on a microtome. The
sections were stained with hematoxylin (H&E). Results were evaluated with
light microscope.
In control group
normal gill histology was observed. In 0.2 mM exposure group, apical fusion,
hyperplasia at primary lamellae and degeneration at secondary lamellae were
detected. In 0.4 mM exposure group, hyperplasia at both primary and secondary
lamellae, fusion at secondary lamellae, hypertrophy at secretory cells and
vacuolization at primary lamellae were monitored. In 0.6 mM exposed group,
severe vacuolization at secretory cells, hyperplasia at both primary and
secondary lamellae, vacuolization at primary lamellae and apical fusion were
observed.
Anahtar Kelimeler
Kaynakça
- Cansu AKBULUT, Zeynep İŞEL, Mustafa ZENGİN, Nazan Deniz YÖN
- Sakarya University Science and Letters Faculty Department of Biology, Sakarya, Turkey Sakarya University Science and Letters Faculty Department of Chemistry, Sakarya, Turkey
Öz
Kaynakça
- Cansu AKBULUT, Zeynep İŞEL, Mustafa ZENGİN, Nazan Deniz YÖN
- Sakarya University Science and Letters Faculty Department of Biology, Sakarya, Turkey Sakarya University Science and Letters Faculty Department of Chemistry, Sakarya, Turkey
Ayrıntılar
| Yazarlar | |
|---|---|
| Yayımlanma Tarihi | 16 Şubat 2017 |
| Yayımlandığı Sayı | Yıl 2017 Cilt: Volume 2 Sayı: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology |