LETHAL MUSHROOM TOXINS: ANALYSIS OF THE AMANITINS AND APPLICATION OF LATERAL FLOW IMMUNOASSAY

Yıl 2017, Cilt: Volume 2 Sayı: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology, 7 - 7, 16.02.2017

Ertuğrul Kaya

Öz

Deaths from mushroom poisoning, due to the ingestion
ofmushrooms containing amatoxins, seem to be increasingboth in Turkey and
worldwide. The amatoxinsfound in Amanita phalloides (also called thedeath cap
mushroom) are most toxic agents, and they are responsible for more than 95% of
the cases of deadlymushroom poisoning. Alpha amanitin is best known toxin of
this group. Alpha amanitin analysis can be carried out with special methods
such as chromatography and ELISA. ELISA method is suitable in order to analyse
urine and blood containingfor alpha amanitin. Gas chromatography system is not
suitable to analyse for alpha amanitin. HPLC and LC-MS/MS systems are best
analytic methods for all materials containing alpha amanitin.

Some analytical machines, supplies and experienced personal
are required to perform these analytical methods. There is a need for more
simple analytical method. The lateral flow immunoassay method may be an easy
solution for this purpose. The lateral flow immunoassays are commonly used
worldwide for different purposes such as pregnancy test and drug screening
test. This test works based on antigen-antibody complexes that is coupled with
visible molecules. These tests are theoretically possible for every analyte
whose antibody is producible, and by changing the antibody concentrations, the
sensitivity of these tests can be adjusted to the desired thresholds.

Analysis of the amanitins and application of alpha amanitin
to lateral flow immunoassay will be declarate in this speech.

Anahtar Kelimeler

LETHAL MUSHROOM TOXINS: ANALYSIS OF THE AMANITINS AND APPLICATION OF LATERAL FLOW IMMUNOASSAY

Kaynakça

• Ertuğrul KAYA
• Department of Pharmacology, Düzce University School of Medicine, Turkey