EVALUATION OF CYTOCHROME P450 2C19 ENZYME ACTIVITY IN PATIENT WITH ANKYLOSING SPONDYLITIS

Yıl 2017, Cilt: Volume 2 Sayı: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology, 47 - 47, 16.02.2017

Mustafa Tugrul Goktas Erdem K. Özer Halil Kara İlknur Albayrak Gezer Umit Yasar

Öz

Ankylosing spondylitis (AS) is a chronic autoimmune disease which is
characterized by the increase of the proinflammatory cytokines (TNF-α, IL-6,
IL-17 and IL-23). Some studies have indicated that the enhanced levels of the
proinflammatory cytokines at the same time downregulate cytochrome P450
enzymes. In a previous study, we found that CYP2C19 activity was lower in
Behçet's patients compared to healthy volunteers. The purpose of this
study was to determine the CYP2C19 enzyme activity in the Turkish ankylosing
spondylitis (AS) patients and healthy volunteers.

Thirty-two AS patients (19 male, 13
female) and 51 healthy volunteers (31 male, 20 female) were recruited in the
study. Three hours after a single oral dose of lansoprazole (30 mg), drug and
its metabolite 5-hydroxy lansoprazole was determined by HPLC. Differences in
metabolic rate of lansoprazole were compared with two-tailed Mann–Whitney U
test.

The average of lansoprazole metabolic ratio
(MR; lansoprazole/5-hydroxy lansoprazole) in AS patients was
1.4 fold higher as compared to the healthy volunteers group (p**= 0.006, median
and 95% CI: 20.2 [17.2-27.3] and 10.1 [9.9-20.8],
respectively).

 

 

 

 

 

 

CYP2C19 enzyme activity was significantly
lower in the AS patients as compared to the healthy volunteers. The decrease
CYP2C19 enzyme activity may be releated with the inceased production of the
inflammatory cytokines in AS patients. This work supports the results that we
obtained from patients with Behcet disease. These findings have significant
inferences for the drug toxicity or unresponsiveness to treatment of CYP2C19
substrates in AS patients.

Anahtar Kelimeler

EVALUATION OF CYTOCHROME P450 2C19 ENZYME ACTIVITY IN PATIENT WITH ANKYLOSING SPONDYLITIS

Kaynakça

• Yildirim Beyazit University, Faculty of Medicine, Department of Pharmacology, Ankara, Turkey
• Selçuk University, Faculty of Medicine, Department of Pharmacology, Konya, Turkey
• Selçuk University, Faculty of Medicine, Department of Physical Medicine and Rehabilitation, Konya, Turkey
• Hacettepe University, Faculty of Medicine, Department of Pharmacology, Ankara, Turkey