DOES FIPRONIL HAS THE NEUROTOXIC EFFECT ON NEUROBLASTOMA SH-SY5Y CELL LINE?

Yıl 2017, Cilt: Volume 2 Sayı: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology, 132 - 132, 16.02.2017

Özge Nur Kanat , Güldeniz Selmanoğlu

Öz

In our country,
fipronil, an insecticide that is used to spraying mainly cereals, maize and
sunflower, causes negative effects on nervous system. It is known that the
insecticide causes changes in the number and morphology of nerve cell.

In the present
study, it was investigated the cytotoxic effect of fipronil in SH-SY5Y
neuroblastoma cell line. Accordingly, SH-SY5Y cells exposed to 125, 250 and 500
µM fipronil doses and incubated for 24 and 48 hours. The cytotoxicity of the
fipronil was analysed by MTT
((3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) test, as an in
vitro cell growth inhibition assay and LDH (Lactate dehydrogenase) assay, to
determine the cell membrane damage. Experiments have been triplicated and the
cell cytotoxicity was defined as% in all group.

The MTT assay
results revealed that fipronil significantly reduced cell viability in a
concentration dependent manner. Cell death increased apparently time dependent
manner in all doses group, especially 500 µM fipronil.

Release of LDH
was detected in the culture medium. The LDH assay results revealed that
fipronil significantly increased cytotoxicity in a concentration dependent
manner. Cell viability was significantly diminished in all doses group,
especially 500 µM fipronil.

We observed that
the results of both tests are consistent with each other. According to the
results; toxicity increases depending on time and dose manner. After this
preliminary study, further detailed investigation will be performed.

Anahtar Kelimeler

DOES FIPRONIL HAS THE NEUROTOXIC EFFECT ON NEUROBLASTOMA SH-SY5Y CELL LINE?

Kaynakça

• Özge Nur KANAT, Güldeniz SELMANOĞLU Hacettepe University, Faculty of Science, Department of Biology, Beytepe Campus, 06800 Ankara, Turkey.