A DOSE–RESPONSE STUDY FOLLOWING IN VIVO EXPOSURE TO DI (2-ETHYLHEXYL)-PHTHALATE (DEHP): HISTOLOGIC, IMMUNO HISTOCHEMICAL, MORPHOMETRIC AND HORMONAL EFFECTS ON REPRODUCTIVE ORGANS IN PUBERTAL MALERATS

Yıl 2017, Cilt: Volume 2 Sayı: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology, 147 - 147, 16.02.2017

Gözde Karabulut Nurhayat Barlas

Öz

The aim of this
study was to investigate the effect of DEHP on there productive system of
malerats. Pubertal 24 male Wistar albino rats were exposed to 100,200 and 400
mg/kg/day of DEHP by gavage for 28 days and at the end of the study, testes,
epididiymis, seminal vesicle and prostate gland were taken and investigated
histopathologically. Also, in serum, testosterone was measured with
testosterone kit. Seminiferous tubules of testis were scored for tubule
diameter, lumen diameter and height of tubule epithelium. In testis, the Tunel
method was applied and evaluated.

There was no
significant difference between first and final body weights. In 200 and 400
mg/kg/day of DEHP, absolute right and left testes weights were decreased. DEHP
exposure caused congestion, degeneration of tubules and tubular atrophy in
testis, tight lumens of tubules, tubuleswithout sperm andless sperm in thelumen
in epididiymis, atrophic tubules and prostatic intraepithelial neoplasia in
prostate and decrement of secretion and cells in the lumen in seminal vesicle.
The testosterone was decreased in all treatment groups compared to the control
group. There were shown apoptotic cells in the lumen of testes in dose groups
with using Tunel method. Luminal diameter and tubular diameter were increased
in 100 mg/kg/day of DEHP dose group compared to the other groups statistically
significant. Epithelial length had the highest level in 400 mg/kg/day of DEHP
dose group.

This study showed that DEHP has side effects on male reproductive system
histologically and hormonal.

Anahtar Kelimeler

A DOSE–RESPONSE STUDY FOLLOWING IN VIVO EXPOSURE TO DI (2-ETHYLHEXYL)-PHTHALATE (DEHP): HISTOLOGIC, IMMUNO HISTOCHEMICAL

Kaynakça

• Gözde KARABULUT, Nurhayat BARLAS
• Hacettepe University, Science Faculty, Department of Biology, 06800, Beytepe Campus, Ankara, Turkey