BIODEGRADATION OF HEPTACHLOR BY PHANEROCHAETE CHRYSOSPORIUM ME446: TOXIC EFFECTS OF HEPTACHLOR AND ITS METABOLITES ON MICE

Yıl 2017, Cilt: Volume 2 Sayı: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology, 161 - 161, 16.02.2017

Münevver Arısoy

Öz

Many organic
pollutants like heptachlor, persist in the environment. These toxic and
canserogenic pollutants tend to the accumulate in the body fat of organisms.
Inthis study the physiological conditions which cause the degradation of
heptachlor (1,4,5,6,7,8,8-heptachloro -3a, 4,7,7a-tetrahydro—4,7-methanoindene)
by Phanerochaete chrysosporium ME446, a white- rot fungus, were determined.

For the degradation
of heptachlor the optimum pH was found to be 5, incubation period 20 days, and
incubation tempruture 30-350C, while the optimum concentration was 50µM. The
maximum heptachlor degradation rate under optimum physiological conditions was
found to be 97.30 %.

Following the
identification of the LD50 dose of heptachlor, its effects on cells in the
blood of mice were investigated toxicological evaluations. The LD50 dose of
heptachlor was found to be 97.50 mg/kg. Although this dose normally causes
death in mice at a rate of 50%, under the same physological conditions, the
resuldant methabolites obtained through Phanerochaete chrysosporium ME446
treatment caused no deaths. On the other hand, it was observed that when direct
application of heptachlor produced quantative effects in the blood cells, no
significant effects of metabolites were seen.

Anahtar Kelimeler

BIODEGRADATION OF HEPTACHLOR BY PHANEROCHAETE CHRYSOSPORIUM ME446: TOXIC EFFECTS OF HEPTACHLOR AND ITS METABOLITES ON MICE

Kaynakça

• Münevver ARISOY
• Ankara University, Faculty of Health Sciences, Department of Nutrition and Dietary, Altındag, Ankara, Turkey.