SYNTHESIS, CHARACTERIZATION, ANTIMICROBIAL ACTIVITY, ANTIMUTAGENIC EFFECT AND DNA BINDING STUDIES OF 2-HYDROXY SCHIFF BASES

Yıl 2017, Cilt: Volume 2 Sayı: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology, 168 - 168, 16.02.2017

Bahar Gök Neslihan Demır Mustafa Yıldız Pınar Gök

Öz

Schiff bases
have been reported for their biological activities. They have been widely used
ligands, due to their metal complexes having variety of applications in
anticancer, antibacterial drugs, catalysis, functional materials, and optical
resolution. The DNA binding, cytotoxicity and apoptosis induction activity were
studied for Schiff bases. They have been widely used in the pharmaceutical
industry, biological research in the medical field, and agriculture. In this
study, synthesis, characterization, antimicrobial activity, antimutagenic, DNA
cleveage and DNA binding properties of 2-hydroxy Schiff Bases.

The Schiff bases
prepared from the reaction of 2-hydroxybenzaldehyde with substitue anilines. To
determine the antimicrobial activity of 2-hydroxy schiff bases Escherichia
coli, Pseudomonas aerouginosa, Proteus vulgaris, Staphylococcus aureus,
Enterococcus faecalis, Bacillus subtilis, Candida albicans ve Candida
tropicalis. Gentamicin, ampicillin and fluconasol were used as controls in this
study. We prepared solutions of 2-hydroxy Schiff bases at 500, 50, 5 and 0.5
ppm concentrations. For Ames test, Salmonella thyphimurium TA98 and TA100
mutant srains were used.  The potential
binding ability of the Schiff base to calf tymus DNA (CT-DNA) was characterized
by UV-vis spectroscopy. Also, DNA cleavage activity of the compounds were
studied by agarose gel electrophoresis.

As a result, the
Schiff bases were active against of yeasts and as well as active against
bacteries. The compounds have intercalative binding, and antimutagenic effects.

Anahtar Kelimeler

SYNTHESIS, CHARACTERIZATION, ANTIMICROBIAL ACTIVITY

Kaynakça

• Bahar GÖK, Neslihan DEMIR, Mustafa YILDIZ, Pınar GÖK
• Çanakkale Onsekiz Mart University, Graduate School, Department of Biology 17100 Çanakkale Çanakkale Onsekiz Mart University, Faculty of Arts and Sciences, Department of Biology, 17100 Çanakkale Çanakkale Onsekiz Mart University, Faculty of Arts and Sciences, Department of Chemistry, 17100 Çanakkale