ASSESSMENT OF IN VITRO GENOTOXICITY AND ANTIGENOTOXICITY OF LUTEOLIN BY USING COMET ASSAY

Yıl 2017, Cilt: Volume 2 Sayı: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology, 174 - 174, 16.02.2017

Esra Erıkel Deniz Yüzbaşıoğlu Fatma Ünal

Öz

Luteolin is a
flavone which occurs in medicinal plants as well as in some vegetables and
spices. Celery, green pepper, carrots, olives and artichokes are major
nutritional luteolin sources. Many studies have demonstrated that luteolin has
numerous biological activities such as anti-oxidant, anti-inflammatory,
cardioprotective, anti-diabetic, anti-allergic and anti-cancer. The purpose of
this study is to investigate genotoxic and antigenotoxic effect of luteolin
against H₂O₂ induced DNA damage by using comet assay in
human lymphocyte in vitro. The lymphocytes isolated from 2 healthy volunteer (1
male and 1 female) were incubated with five different concentrations of
luteolin (0.39, 0.78, 1.56, 3.12 and 6.25 µg/mL) alone and simultaneously with
H₂O₂ (100 mM) at 37^oC for 1 hour. A negative
(distilled water), a solvent (50% methanol) and a positive control (H2O2) were
also maintained. A total of 200 cells were evaluated per concentration for tail
intensity (%), tail length (μm), and tail moment by using Comet Assay IV,
Perceptive Instruments Ltd., UK. Luteolin alone did not induce significant DNA
damage in all the concentrations compared to negative and solvent controls.
Luteolin+ H₂O₂ treatment significantly reduced DNA damage
at all the concentrations compared to H₂O₂ treatment
alone (positive control) for all comet parameters. Our results have indicated
that luteolin exhibited chemopreventive activity against DNA damage induced by
H₂O₂ which has oxidative effects.

Anahtar Kelimeler

ASSESSMENT OF IN VITRO GENOTOXICITY AND ANTIGENOTOXICITY OF LUTEOLIN BY USING COMET ASSAY

Kaynakça

• Esra ERIKEL, Deniz YÜZBAŞIOĞLU, Fatma ÜNAL
• Gazi University, Science Faculty, Department of Biology, Genetic Toxicology Laboratory, 06500, Ankara, TURKEY