HYPERICIN INDUCED CHANGES IN CYTOTOXICITY AND METABOLIC ACTIVITIES IN HT-29 ADENOCARCINOMA CELLS

Yıl 2017, Cilt: Volume 2 Sayı: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology, 192 - 192, 16.02.2017

Aysun Kılıç Süloğlu Güldeniz Selmanoğlu

Öz

Photodynamic
therapy (PDT) is an alternative way to the most common cancer treatment
methods. The use of PDT as a cancer therapy is particularly attractive because
of its selectivity on cancer cells. Hypericin (HYP) which is derived from
Hypericum perforatum, continues to be one of the most promising
photosensitizer, as it fascinates with its antitumor, antiviral and
antidepressant properties. In this study, HT-29 (grade-1) human colon
adenocarcinoma cells treated with 0.04 μM 0.08 μM or 0.15 μM HYP concentrations
and after 24 hours cells were irridated with fluorescent lamps. The effects of
HYP examined 16 and 24 hours after the activation. The effect of HYP on cell
viability analysed with trypan blue staining and MTT tests. Besides, amounts of
glucose, lactate and activity of lactate dehydrogenase (LDH) were measured for
the metabolic activity. According to the results, in HT-29 cells as HYP
concentration increased cytotoxicity increased. While glucose consumption and
lactate production decreased, the activity of LDH increased depending on the
HYP concentration. In conclusion, this work demonstrates that HYP-mediated PDT
have a critical role in leading alterations in metabolic pathways depending on
the HYP concentration and incubation time.

Anahtar Kelimeler

HYPERICIN INDUCED CHANGES IN CYTOTOXICITY AND METABOLIC ACTIVITIES IN HT-29 ADENOCARCINOMA CELLS

Kaynakça

• Aysun Kılıç SÜLOĞLU, Güldeniz SELMANOĞLU
• Department of Biology, Faculty of Science, Hacettepe University, 06800, Beytepe Campus, Ankara, Turkey