DETECTION OF CATHINONES IN URINE BY LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY WITH A VALIDATED METHOD

Yıl 2017, Cilt: Volume 2 Sayı: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology, 221 - 221, 16.02.2017

Mukaddes Gürler Pınar Taşkın Ebru Özcan Şahin Karkın

Öz

Cathinone is the
principal active constituent of the Khat plant (Catha edulis), and has similar
stimulant properties to natural amphetamine. Khat leaves are chewed as a
recreational drug especially in Arabian Peninsula and East Africa.
Internationally, cathinone is a Schedule I drug under the Convention on
Psychotropic Substances. Substituted cathinones are derivatives of cathinone;
some of them have medical uses as well, but some are strong psychoactive drugs
and commonly sold in “bath salts”. Their use may have very serious public
health and safety consequences. Therefore, testing for these drugs is an
important issue for laboratories. Their potential for thermal instability
during gas chromatography- mass spectrometry and the need for sensitivity in
forensic toxicology determinations liquid chromatography-tandem mass
spectrometry LC-MSMS is preferable for the determination of synthetic cathinones
in biological samples.

Our aim was to
develop a validated LC-MSMS method for determining synthetic cathinones
(Methedron, Buphedron, Mephedron, Alpha-PVP, Bupropion, d,I-4-etilmetcathinon)
in human urine samples. For this purpose, we used certified standard materials
with an internal standard solution and gradient grade chemicals. We studied the
linearity, LOD, LOQ, accuracy and imprecision, repeatability, reproducibility,
recovery and carry-over as validation parameters of the assay. Tandem mass
spectrometric detection at positive electrospray ionization in the MRM mode was
used. The results indicated that all parameters were in acceptable analytical
ranges. This validated method is suitable for analyzing cathinones in clinical
and forensic toxicology cases.

 

Anahtar Kelimeler

DETECTION OF CATHINONES IN URINE BY LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY WITH A VALIDATED METHOD

Kaynakça

• Hacettepe Üniversity Medical Faculty, Forensic Toxicology Laboratory