BISPHENOL A LEADS TO NECROPTOSIS IN SH-SY5Y NEUROBLASTOMA CELLS

Yıl 2017, Cilt: Volume 2 Sayı: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology, 231 - 231, 16.02.2017

Beyza Ayazgök Tuba Tüylü Küçükkılınç

Öz

Bisphenol-A is a
entensively used phenolic chemical in the production of polycarbonate plastic
and epoxy resins. BPA could strip from water pipes, food and beverage containers,
eyeglasses lenses, dental monomers. In this way general population is
continually and unavoidably exposed to BPA from several sources.

We have
previously reported that low dose BPA induce cell death in SH-SY5Ycells. In this
study we aimed to examine necroptotic effects of BPA in SH-SY5Y cells. SH-SY5Y
cells were seeded at 300.000 cells per well in 6-well plates and cultured in
DMEM at 37°C with 5% CO2 and treated with 1 nM of BPA and 50 µM Necrostain
1(RIPK1 inhibitor) dissolved in DMSO. After 48 hours, samples were pooled then
washed with PBS. A 100 μL aliquot of cells from each sample was centrifuged at
12000 g, +4 ºC for 2 minutes and resuspended in 100 μL annexin binding buffer
added to 5 μL Annexin V. Then, samples were centrifuged and resuspended again
in 100 μL of the same buffer and added with 1 μL PI at room temperature
protected from light and analysed at Tali® Image-Based Cytometer. Our results
suggest that 1 nM BPA leads to necrosis in SH-SY5Y cells at 48 hours. Necrostatin-1
is also used to indicate whether BPA induced necrosis in neuroblastoma cells is
programmed. Treatment with Nec-1 significantly switched necrotic cell death to
apoptotis. Consequently BPA induces RIPK-1 dependent necroptosis of SH-SY5Y
cells and has a important role in the initiation of necrosis.

 

Anahtar Kelimeler

BISPHENOL A LEADS TO NECROPTOSIS IN SH-SY5Y NEUROBLASTOMA CELLS

Kaynakça

• Beyza AYAZGÖK, Tuba Tüylü KÜÇÜKKILINÇ
• Hacettepe University Faculty of Pharmacy Department of Biochemistry