FAST QUANTITATION OF NORTRIPTYLINE BY LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY (LC-MS/MS)

Yıl 2017, Cilt: Volume 2 Sayı: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology, 298 - 298, 16.02.2017

Abdullah Sıvrıkaya Sedat Abusoglu , Ali Unlu

Öz

Therapeutic drug
monitoring (TDM) of tricyclic
antidepressants (TCAs) is considered
useful in patients with major depressive disorder, since these drugs display
large individual differences in clearance, and the therapeutic windows of these
drugs are relatively small. Nortriptyline is a second-generation tricyclic
antidepressant and used in the relief of symptoms of depression and in some
cases of nocturnal enuresis. The aim of this study was to develop a simple,
fast and accurate tandem mass method for determination and quantification of
nortriptyline. This method is designed for high sample throughput of only 10µL
serum sample.

Mass
spectrometric analyses were performed using a Shimadzu LC-20-AD (Kyoto, Japan) coupled with a ABSCIEX API 3200 triple
quadrupole mass spectrometer (USA)
equipped with an electrospray ion source (ESI)
operating in positive mode.

The standard
curves for nortriptyline was linear within the range of 1,56-100 µg/L. Total
run time was 5 minutes. Chromatographic separation was performed on a C18
column (4.6×50 mm, 5 µm, Phenomenex Luna)
with a mobile phase consisting of 1% formic acid in water and MeOH (10:90, V/V) at a flow rate of 0.2 ml/min.

The narrow
therapeutic range of these drugs are of importance to be analyzed at critical
concentrations. This method has capability to determine these levels.

Data from
calibration curves reveal that the method is accurate and precise.

Anahtar Kelimeler

FAST QUANTITATION OF NORTRIPTYLINE BY LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY (LC-MS/MS)

Kaynakça

• Abdullah SIVRIKAYA, Sedat ABUSOGLU, Ali UNLU
• Department of Biochemistry, Selcuk University Faculty of Medicine, Konya, Turkey