GENOTOXIC PROPERTIES OF SOME N-PHENYLBENZAMIDE AND N-PHENYLACETAMIDE DERIVATIVES

Yıl 2017, Cilt: Volume 2 Sayı: İssue 1 (1) - 2.İnternational Congress Of Forensic Toxicology, 305 - 305, 16.02.2017

Fatma Zilifdar Egemen Foto Tugba Ertan Bolelli Esin Akı İsmail Yalçın Nuran Diril

Öz

Benzamide and
acetamide derivatives exhibit various types of biological properties such as
antihelmentic, antihistaminic, antifungal, antibacterial and anticancer. We
previously synthesized 12 N-phenyl benzamid and N-phenylacetamide derivatives
which might have anticancer activity on account of their heterocyclic structure
and evaluated anticancer activity on cancer cells. They exhibited significant
cytotoxic activity on HeLa cells. However, it is well known that genotoxicity
posed by chemotherapy is a major concern since it induces DNA damage and
instability in the patients’ genome. 
Therefore, the genotoxic properties of this series were investigated by
a prokaryotic and an eukaryotic test systems in this study.

Due to the fact
that each genotoxicity tests are based on different cellular mechanism,
genotoxic potentials of chemicals should be assessed with battery test systems.
Therefore, we performed alkaline Comet assay on L929 cells and microplate
technique of Bacillus subtilis spore Rec assay. All determinations were made in
triplicate. Results were evaluated with T-test and S-probit analyze for comet
assay and rec assay, respectively with the confidence interval 95-99%.

According to the
results, only two compounds (c-8 and c-11) exhibited genotoxic activity and 7
compounds had no genotoxic potentials at both tests. However, the results of
both genotoxicity tests for 3 compounds (c-5, c-6, c11) were not parallel.
Though their genotoxic potential should be tested with other assays, compounds
founded non-genotoxic for both tests in this study are promising for their
anticancer activity.

Anahtar Kelimeler

GENOTOXIC PROPERTIES OF SOME N-PHENYLBENZAMIDE AND N-PHENYLACETAMIDE DERIVATIVES

Kaynakça

• Fatma ZİLİFDAR, Egemen FOTO, Tugba Ertan-BOLELLİ, Esin AKI, Ismail YALÇIN, Nuran DİRİL
• Molecular Biology Department, Faculty of Science, Hacettepe University, Beytepe, Ankara, Turkey Pharmaceutical Chemistry Dept Faculty of Pharmacy, Ankara University, Tandogan, Ankara, Turkey