Investigation and comparison of antimicrobial and disinfectant resistance in different serotypes of Salmonella isolated from poultry in Aegean region

Yıl 2017, Sayı: Issue 1(2) - 3. INTERNATIONAL BIOCIDAL CONGRESS, 6 - 6, 01.02.2017

İhsan Yasa Nazenin Eftekhari Belgin Umay

Öz

Aims: Poultry are the
major food-producing animals in several countries and have been implicated as
sources of antimicrobial-resistant Salmonella. The susceptibility to
disinfectants has been less reported and very little is known about
disinfectants which are used as food preservative. The aims of the present
study was to investigate the prevalence of antibiotic resistance and compare it
with the susceptibility of selected disinfectants in Salmonella enterica serotypes
isolated from poultry in Aegean region. Methods-Results: A total of 50 Salmonella
enterica isolates from poultry were included in this study. Salmonella
enterica infantis (30%) and Salmonella enterica enteritidis (14%)
were the most frequent serogroups in this study. Antimicrobial susceptibility
testing was performed according to the Clinical and Laboratory Standards
Institute (CLSI) guidelines. Presence of class 1 integron among the isolates
was investigated using primers specific conserved region. Different resistance
gene arrays were identified among class 1 integrons. Aminoglycoside
adenyltransferase (aadA1) and aminoglycoside acetyltransferase (aacA4) gene
cassettes was detected in all of isolates. Six different
disinfectants;chlorhexidine gluconate 20% (CHX), benzalkonium chloride 50%
(BC), 1,2 benzisothiazolinon/ 2-methyl-2H isithiazolin-3 one 1.02-1.04 g/ml
(BIT/MIT), 5 chloro 2 methyl -2H isothiazolin-3 one/ 2-methyl-2H isothiazolin-3
one + Broponol 1.05-1.07 g/ml (CIT/MIT+Broponol), 5 chloro 2 methyl -2H
isothiazolin-3 one/ 2-methyl-2H isothiazolin-3 one 1.02 g/ml (CIT/MIT) and
Microcare SB were utilized in this study. All of these disinfectants were used
as preservative in food industry. The isolates showed a high susceptibility to
CIT/MIT, MIT/BIT, CIT/MIT+Bropanol, CHX, BTC and a low susceptibility to
Microcare SB. The minimal inhibitory concentrations (MICs) of these
disinfectants were determined. Conclusion: The data obtained in this
study suggested that class 1 integrons are widely distributed among serogroups
of Salmonella enterica and demonstrated disinfectant resistance
serotyped Salmonella. The study shows the need for monitoring the antimicrobial
and disinfectant resistance varied in different Salmonella serotypes.

 

Keywords:
Salmonella
enterica,
integron, disinfectant

 

 

Anahtar Kelimeler

Investigation and comparison of antimicrobial and disinfectant resistance in different serotypes of Salmonella isolated from poultry in Aegean region

Kaynakça

• Ihsan Yasa, Nazenin Eftekhari, Belgin Umay Department of biology, Faculty of Sciences, Ege University, Izmir, Turkey