Molecular phylogeny of Ochlerotatus species inferred from COI sequences

Yıl 2017, Sayı: Issue 1(2) - 3. INTERNATIONAL BIOCIDAL CONGRESS, 31 - 31, 01.02.2017

Nurper Guz Enver Durmuşoğlu

Öz

Accurate
identification of mosquito species is essential in epidemiological studies of
vector-borne pathogens. Among these vectors, Ochlerotatus is a large highly
varied group of cryptic species with similar morphological characters shared by
members of species complexes. Here we presented molecular identification of
three Ochlerotatus species using a short fragment of the mitochondrial
cytochrome c oxidase subunit I (COI) sequence serving as a DNA bio-barcode. The
nucleotide sequence from each specimen was compared with barcode sequences on
NCBI using nucleotide Basic Local Alignment Search Tool (BLASTn). The results
of the BLASTn search in the Genbank data base retrieved highest homology to the
species including Ochlerotatus caspius (99%), Ochlerotatus detritus (98%) and
Ochlerotatus provocans (96%). To our knowledge, this study represents the first
report of the emergence of O. provocans in Muğla Province. Neighbour-joining
trees were drawn based on DNA barcodes and all the specimens were clustered in
agreement with their taxonomic classification at species level. Nucleotide
composition and haplotype diversity of DNA barcodes were also presented in our
study. Furthermore genetic distances, including intraspecific and interspecific
pairwise sequence divergence along with nearest-neighbour distance, were
calculated using the Kimura-2- parameter. As a result data obtained from this
study clearly demonstrate that DNA barcodes could be used as an efficient
molecular tool in identification of especially cryptic species.

 

 

 

Keywords: Cytochrome c oxidase oxidase subunit I, DNA barcoding,
Ochlerotatus sp., Phylogenetic analysis, new record

Anahtar Kelimeler

Molecular phylogeny of Ochlerotatus species inferred from COI sequences

Kaynakça

• Nurper Guz, Enver Durmuşoğlu Ankara University, Insect Molecular Biology Laboratory, Department of Plant Protection, Faculty of Agriculture, Ankara, Turkey 2Ege University, Department of Plant Protection, Faculty of Agriculture, İzmir, Turkey