MASS SPECTROMETRIC QUANTITATION OF SERUM CEPHAZOLIN

Yıl 2017, Volume 2, Issue 1(4), 29 - 29, 01.11.2017

Ali Unlu Sedat Abusoglu Abdullah Sivrikaya

Öz

Objectives

Cefazolin, a first generation
cephalosporin β-lactam antibiotic,
is commonly used for prophylaxis during cesarean delivery, and in treatment of
pulmonary infections and many surgical procedures. The aim of this work was to
develop a simple, fast and accurate liquid-chromatography-mass spectrometry method
for determination of serum cefazolin.

 

Methods

Mass spectrometric analyses were
performed using an Shimadzu LC-20-AD (Kyoto, Japan) coupled with a ABSCIEX API
3200 triple quadrupole mass spectrometer (USA) equipped with an electrospray
ion source (ESI) operating in positive mode.

Results

The standard curves for cefazolin
was linear within the range of 0.39-200 µg/ml. Total run time was 5 minutes. Chromatographic
separation was performed on a C18 column (4.6×50 mm, 5
µm, Phenomenex Luna) with a mobile phase consisting of 1%
formic acid in water and MeOH (10:90, V/V)
at a flow rate of 0.8 ml/min.

 

Discussion

Serum cefazolin measurement can
be easily performed by LC-MS/MS system to identify the risk of the patients
with therapeutic drug monitoring. This method performs this drug analysis with
high throughput.

 

Conclusions

Exact determination of cefazolin
provides reliable levels in clinical studies to avoid toxic effects.

 

Keywords

Antibiotic levels, mass
spectrometry, cefazolin, toxicity.

 

Acknowledgments: No conflict
of interest

Anahtar Kelimeler

MASS SPECTROMETRIC QUANTITATION OF SERUM CEPHAZOLIN

Kaynakça

• Ali Unlu*, Sedat Abusoglu, Abdullah Sivrikaya
• Department of Biochemistry, SelcukUniversity Faculty of Medicine, Konya, Turkey