Variation in Heat Stress-Induced Some Physiological Changes and Peroxidase Activities Among Three Tomato (Lycopersicon esculentum Mill.) Cultivars

Year 2014, Volume: 1 Issue: Özel Sayı-2, 1492 - 1498, 01.03.2014

Ece Turhan Cigdem Aydogan Sergul Ergın Nilufer Ozturk

Abstract

Variation in heat stress-induced some physiological changes and peroxidase (POX) activities were studied in three tomato (Lycopersicon esculentum Mill.) cultivars, Çaltı, Pembe and Yaren. For this purpose, the leaves were collected from tomato plants at the first bloom and yield stages. The leaves were subjected to heat stress treatments in water bath at 35, 40, 45, 50, 55 and 60 °C with gradual increments every half an hour. The leaves were then analysed for ion leakage, loss of turgidity, soluble peroxidase (S-POX) and cell wall-bound peroxidase (CWB-POX) activities. In general, effects of heat stress on the variables studied were significant. Results revealed that ion leakage and loss of turgidity were increased parallel to the temperatures. In addition, loss of turgidity values were higher in the yield stage than those in the first bloom stage. Considering the POX activities, the SPOX activity was greater in the first bloom stage than in the yield stage in all cultivars. Moreover, the highest and the lowest enzyme activity were detected in cvs. Yaren and Pembe, respectively. In contrast to S-POX activity, the CWB-POX activity was greater in the yield stage than in the first bloom stage in cvs. Çaltı and Pembe. The highest CWB-POX activity was detected in cv. Çaltı while the lowest activity was detected in cv. Pembe. Data also indicated that, generally CWB-POX activity was high in the samples in response to heat stress treatments

Keywords

Tomato, heat stress, ion leakage, loss of turgidity, peroxidase

Variation in Heat Stress-Induced Some Physiological Changes and Peroxidase Activities Among Three Tomato (Lycopersicon esculentum Mill.) Cultivars

Abstract

Çaltı, Pembe ve Yaren domates (Lycopersicon esculentum Mill.) çeşitlerinde sıcaklık stresinin teşvik ettiği bazı fizyolojik değişiklikler ve peroksidaz (POX) aktivitelerindeki farklılıklar belirlenmiştir. Bu amaçla domates bitkilerinden ilk çiçeklenme ve verim dönemlerinde yaprak örnekleri toplanarak, su banyosunda sıcaklık kademeli olarak arttırılarak 35, 40, 45, 50, 55 ve 60 °C sıcaklıklarda, her sıcaklık kademesinde 30 dakika tutulmuştur. Yaprak örneklerinde iyon sızıntısı, turgor kaybı, çözünebilir peroksidaz (S-POX) ve hücre duvarı peroksidaz (CWB-POX) aktiviteleri belirlenmiştir. Genel olarak sıcaklık stresinin incelenen parametreler üzerindeki etkileri önemli bulunmuştur. Sonuçlar, iyon sızıntısı ve turgor kaybı değerlerinin sıcaklıklara paralel olarak arttığını göstermiştir. Bunun yanı sıra turgor kaybı değerleri verim döneminde ilk çiçeklenme dönemine göre daha yüksek bulunmuştur. POX aktiviteleri değerlendirildiğinde, S-POX aktivitesinin tüm çeşitlerde ilk çiçeklenme döneminde verim dönemine oranla daha yüksek olduğu tespit edilmiştir. Diğer taraftan en yüksek ve en düşük S-POX aktivitesi sırasıyla Yaren ve Pembe çeşitlerinde olmuştur. CWB-POX aktivitesi ise S-POX aktivitesinin aksine verim döneminde ilk çiçeklenme dönemine oranla daha yüksek bulunmuştur. En yüksek CWB-POX aktivitesinin Çaltı, en düşük CWB-POX aktivitesinin ise Pembe çeşidinde olduğu saptanmıştır. Veriler aynı zamanda CWB-POX aktivitesinin genellikle sıcaklık stresi uygulamasına bağlı olarak arttığını göstermiştir

Keywords

Domates, sıcaklık stresi, iyon sızıntısı, turgor kaybı, peroksidaz

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