Dikegulac-Sodium Effect on Micropropagation and Biochemical Parameters in the Cherry Rootstocks CAB-6P and Gisela 6

Virginia Sarropoulou; Kortessa Dımassıtherıou; İoannis Therıos

Dikegulac-Sodium Effect on Micropropagation and Biochemical Parameters in the Cherry Rootstocks CAB-6P and Gisela 6

Year 2014, Volume: 1 Issue: Özel Sayı-2, 1713 - 1726, 01.03.2014

Virginia Sarropoulou Kortessa Dımassıtherıou İoannis Therıos

Abstract

The effects of dikegulac-sodium (DS) alone and in combination with benzyladenine (BA) οn the morphogenetic and biochemical responses in the cherry rootstocks CAB-6P (Prunus cerasus L.) and Gisela 6 (Prunus cerasus x Prunus canescens) were investigated. In the CAB-6P rootstock, DS did not promote shoot proliferation whereas its application at 40, 120 and 150 μΜ suppressed shoot length. In the Gisela 6 rootstock, the number of shoots per explant and shoot proliferation rate were greater with 80 μΜ DS. Furthermore, DS significantly stimulated rhizogenesis in both rootstocks. Leaf chlorophyll concentration of CAB-6P microshoots was maximum in the control treatment, whereas in the Gisela 6 rootstock, 40 μΜ DS had a positive effect on it. In the CAB-6P explants, DS decreased leaf (20-150 μΜ) and root (80-150 μΜ) carbohydrate concentration as well as proline concentration in roots (40-150 μΜ). All DS concentrations, especially 80 μΜ increased leaf carbohydrate concentration of the Gisela 6 explants. Carbohydrate and proline concentrations in roots were 2.5 times greater with 80 μΜ DS, compared to the control. In the CAB-6P rootstock, a synergistic effect was found between BA and 250 μΜ DS regarding shoot fresh weight (FW). Best rooting results in terms of root number per rooted explant and rooting percentage were obtained with 500 μΜ DS. In the Gisela 6 rootstock, 250 μΜ DS promoted the positive effect of 4.4 μΜ BA concerning shoot number per explant. DS seems to be a promising growth regulator in micropropagation of the cherry rootstocks CAB-6P and Gisela 6

Keywords

Carbohydrates, cherry rootstocks, chlorophyll content, dikegulac-sodium, growth retardants, proline

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