Allelic Variation and Composition for Seed Proteins in Synthetic Hexaploid Wheats (2n=42, BBAuAuDD)

Sonya Doneva; Diana Yordanova; Nadia Daskalova; Penko Spetsov

Allelic Variation and Composition for Seed Proteins in Synthetic Hexaploid Wheats (2n=42, BBAuAuDD)

Year 2014, Volume: 1 Issue: Özel Sayı-2, 1891 - 1897, 01.03.2014

Sonya Doneva Diana Yordanova Nadia Daskalova Penko Spetsov

Abstract

Seven hexaploid synthetic wheats (2n=42, BBAuAuDD), derived by crossing accessions and hybrids from T. turgidum (2n=28, BBAuAu) to diploid Aegilops tauschii (2n=14, DD), following by colchicine treatment for chromosome doubling, were subjected for seed protein analysis. Amphidiploids № 531 and № 107 revealed x-type subunit 1 at the Glu-A1 locus, but four other synthetics (№ 32, 106, 530, 532) expressed subunit 1Ax1.1, uncommon for bread wheat, that could be an example of expanding allelic diversity for HMW-GS along with the D-genome derived genes. At the Glu-B1 locus, five x-type subunits, viz., 7, 13, 17, 14 and 22, and four y-type subunits viz., 8, 16, 18 and 15, and their five combinations were detected. Diversity at the Glu-D1 was found to be greater than the variety of genes contained at Glu-A1 and Glu-B1 loci. In synthetics, the subunit 1Dx1.5+1Dy10 was predominantly observed, which is different from the T. aestivum genome and affect the wheat quality to great extent. Other detected D-genome subunits were 1Dx2+1Dy11 and 1Dx4+1Dy10.1. LMW-GS discovered two different lines in each of № 32, 530 and 532 (with b and c alleles) that was further supported by the gliadin compositions. D-genome synthetic hexaploid wheats seemed to be excellent sources for selecting different glutenin compositions in wheat breeding, as well for gliadins, revealing diverse ω- and γ genes

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References

Branlard, G., Dardevet, M., 1985. Diversity of grain
proteins and bread wheat quality.
Correlation between high-molecular
weight subunits of glutenin and flour
quality characteristics. J Cereal Sci 3: 345- 354.
Ciaffi, M., Margiotta, B., Colaprico, G., De-Stafanis, E., Sgrulletta, D., Lafiandra, D., 1995. Effect of high temperatures during grain filling on the amount of insoluble proteins in durum wheat. J Genet Breed 49: 285-296.
Doneva, S., Spetsov, P., 2011. Polymorphism in glutenins and gliadins in Aegilops tauschii accessions (2n=14, DD). Field Crop Studies, t. VII-2: 243-254 (in Bulg.).
Doneva, S., Spetsov, P., 2013. Protein compositions in three synthetic hexaploid wheats (2n=42, ВВAUAUDD). Annual scientific book of TU–Varna, t. 2: 23-29 (in Bulg.).
Gupta, R.B., Shepherd, K.W., 1988. Low-molecular glutenin subunits in wheat: their variation, inheritance and association with bread- making quality. Proc of 7th Int. Wheat Genet. Symposium, Cambridge, UK, 13-19 July: 943-949.
Gupta, R.B., Shepherd, K.W., 1990. Two-step one- dimensional SDS-PAGE analysis of LMW subunits of glutenin. 1. Varation and genetic control of the subunits in hexaploid wheats. Theor Appl Genet 80: 65-74.
Jackson, E.A., Morel, M.H., Sontag-Strohm T., Branlard, G., Metakovsky, E.V., Redaelli, R., 1996. Proposal for combining the classification system of alleles of Gli-1 and Gli-3 loci in bread wheat ( Triticum aestivum L.). J Genet & Breed 50: 321-336.
Khan, K., Mcdonald, C.E., Banasik, O.J., 1983. Polyacrylamide gel electrophoresis of gliadin identification – procedural modifications variety and observations. Cereal Chem 60: 178- 181.
Laemmli, U. K., 1970. Clavage of structural proteins
during the assembly of the head of
bacteriophage T4. Nature, vol. 227: 680 – 685.
Lafiandra, D., D’Ovodio, R., Porceddu, E., Margiotta, B., Colaprico, G., 1993. New data supporting high mol glutenin subunit 5 as the determinant of quality differences among the pairs 5+10 and 2+12. J Cereal Sci 18: 197 – 205.
Lagudah, E. S., MacRitchie, F., Halloran, G.M., 1987. The influence of high – molecular weight subunits of glutenin from T. tauschii on flour quality of synthetic hexaploid wheat. J Cereal Sci 5: 129 – 138.
Li, Y., Chengyan, H., Xinxia, S., Qiunqi, F., Genying, L., Xiusheng, C., 2009. Genetic variation of wheat landraces contributions improvement in China. Euphytica 169: 159- 168. between varieties to quality wheat
Metakovsky, E.V., 1991. Gliadin allele identification in common wheat. II. Catalogue of gliadin alleles in common wheat. J Genet & Breed 45: 325-344.
Payne, P.I., 1987. Genetics of wheat storage proteins and the effect of allelic variations on bread – making quality. Ann. Rev. Plant Physiol. 38: 141 – 153.
Payne, P.I., Holt, M., Jackson, E.A., Law, C.N., 1984.
Wheat storage proteins: their
genetics and potential for manipulation by