Investigation of the Change in Yield and Bioactivity of Dandelion (Taraxacum officinale) Tincture Depending on Liquid/Solid Ratio and Ethanol Concentration

Year 2018, Volume: 5 Issue: 2, 163 - 174, 06.05.2018

Mahmut Kaplan , Mustafa Mücahit Yılmaz Serap Köprü Hürmet Gözelle Gamze Muhderem Rabia Uslu

https://doi.org/10.30910/turkjans.421360

Cited By: 1

Abstract

In this study, dandelion (Taraxacum
officinale) plant was used for the production of tincture, a hydroalcoholic
extract type, and it was aimed to determine process factor levels for the
optimization of the production conditions in order to increase the final product
quality. For this purpose, central composite rotatable design based on response
surface methodology was utilized for the optimization process. The liquid /
solid ratio and the ethanol concentration were chosen as factors for the
preparation of the samples. In order to determine bioactive properties of samples,
total phenolic content, antiradical capacity (EC₅₀) value and
extract yield properties were determined. Liquid / solid ratio and ethanol
concentration were found to be very effective over the total amount of phenolic
materials. The total phenolic content values ​​of the samples ranged from
605-1754 mg GAE/L, EC₅₀ values ​​ranged from 9.80-199.2, and extract
yields ranged from 2.32-6.49%. Increasing the amount of liquid for herbs
increased the total phenolic content, which led to a decrease in the yield of
the extract. The result of the optimization process showed that the highest
total phenolic content could be achieved in a 1: 4 solid / liquid ratio at a
concentration of 57% ethanol.

Keywords

Dandelion, tincture, bioactivity, optimization, response surface methodology

Sıvı/Katı Oranı ve Etanol Konsantrasyonuna Göre Karahindiba (Taraxacum officinale) Tentürünün Verim ve Biyoaktivitesindeki Değişimin İncelenmesi

Abstract

Bu çalışmada, bir hidroalkolik ekstrakt
tipi olan tentür üretimi amacıyla karahindiba (Taraxacum officinale) bitkisi kullanılmış ve son ürün kalitesini
artırmaya yönelik olarak üretim şartlarını optimize etmek üzere proses faktör
seviyelerinin belirlenmesi amaçlanmıştır. Bu amaçla, optimizasyon işlemi için
tepki yüzey yöntemi tabanlı merkezi tümleşik tasarım dizaynından
faydalanılmıştır. Örneklerin hazırlanması amacıyla sıvı/katı oranı ve etanol
konsantrasyonu faktör olarak seçilmiştir. Örneklerin biyoaktif özelliklerinin
belirlenmesi amacıyla toplam fenolik madde miktarı, antiradikal kapasite ve EC₅₀
değeri ile verim özellikleri hesaplanmıştır. Sıvı/katı oranı ve etanol
konsantrasyonunun örneklerin toplam fenolik madde miktarı üzerinde oldukça etkili
olduğu görülmüştür. Örneklerin toplam fenolik madde miktarı değerleri 605-1754
mg GAE/L aralığında, EC₅₀ değerleri 9.80-199.2 aralığında, ekstrakt
verimleri ise %2.32-6.49 aralığında değişim göstermiştir. Bitki örneklerinde sıvı
miktarının artışı toplam fenolik madde miktarını artırırken ekstrakt veriminde
düşüşe yol açmıştır. Optimizasyon işlemi sonucunda en yüksek toplam fenolik
madde miktarının %57 etanol konsantrasyonunda ve 1:4 katı/sıvı oranında elde
edilebileceği sonucuna ulaşılmıştır.

Keywords

Karahindiba, tentür, biyoaktivite, optimizasyon, tepki yüzey yöntemi

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