Ardahan’da Yetişen Kediotunun (Valeriana officinalis L.) Antimikrobiyal Ve Antioksidan Potansiyelinin Belirlenmesi

Yıl 2019, Cilt: 6 Sayı: 2, 271 - 275, 22.04.2019

Vesile Düzgüner Nurcan Erbil

https://doi.org/10.30910/turkjans.557113

Cited By: 1

Öz

Valerianceae familyasına ait olan kediotu (Valeriana officinalis L.) yerel olarak Avrupa ve Asya’da bulunan çiçekli bir bitkidir ve çoğunlukla uykusuzluk hastalığı ve kaygı bozukluklarının tedavisi için kullanılmaktadır. Bu çalışmada Ardahan yöresinden toplanan kediotunun antioksidan ve antimikrobiyal aktiviteleri incelenmiştir. Kediotunun metanol/ethanol ekstraktlarının antimikrobiyal aktivitesi Pseudomonas aeroginosa (ATCC 9027), Staphylococcus aureus (ATCC 6538), Bacillus megaterium (DSM 32), Escherichia coli, Yarrovia lipolytica, Candida albicans ve Saccharomyces cerevisiae’ya karşı test edilmiştir. Bitkilerin Glutatyon (GSH) düzeyi spektrofotometrik olarak, total oksidan ve antioksidan kapasiteleri (sırasıyla TOK ve TAK) ise ELISA yöntemi ile ölçülmüştür. Kediotunun, bu çalışmada kullanılan test mikroorganizmalarına karşı hafif oranda antibakteriyel aktiviteye sahip olduğu tespit edilmiştir. Metanol ekstraktlarındaki GSH düzeyi etanol ekstraktlarından daha yüksek çıkmıştır. Benzer şekilde total antioksidan kapasite ölçümlerinde methanol ekstraktlarındaki değerler etanol ekstraktlarından fazla saptanmıştır. Sonuç olarak Valeriana officinalis’in yararlı etkilerini belirleyen antibakteriyal ve antioksidatif içeriklerinin etki mekanizması ile ilişkisinin in vitro çalışmalarla desteklenmesi gerektiği kanısına varılmıştır.

Anahtar Kelimeler

Antimikrobiyal, Antioksidan, Glutatyon, Oksidatif Stres, Valeriana Officinalis

Determination of Antioxidative and Antimicrobial Potential of Valerian (Valeriana officinalis L.) Grown in Ardahan Region

Öz

Valerian (Valeriana
officinalis L.) that belongs to Valerianceae
family is a flowering plant native to Europe, and Asia and it is most commonly
used for the treatment of insomnia and anxiety disorders. In this study, the antimicrobial activity and an
antioxidant capacity of valerian collected from Ardahan region were investigated. Methanol/ethanol extracts of
valerian were tested for antimicrobial activity against Pseudomonas aeroginosa (ATCC 9027), Staphylococcus aureus (ATCC 6538), Bacillus megaterium (DSM 32), Escherichia coli, Yarrovia lipolytica, Candida albicans and Saccharomyces cerevisiae.
The measurements of glutathione (GSH) levels were done spectrophotometrically and total oxidant and antioxidant capacity
(TOC and TAC, respectively) were performed by ELISA method. Antibacterial
activity was found at different rate against
test microorganisms used in this study.
Additionally, GSH levels were higher in methanolic extracts than
ethanolic extracts. Similarly, it was determined that TAC in the methanolic extract was much better than
ethanolic extract. In conclusion, it
has been concluded that the mechanism of action of antibacterial and
antioxidative ingredients that determine beneficial effects of Valeriana officinalis should be
supported by in vitro studies.

Anahtar Kelimeler

Antimicrobial, Antioxidant, Glutathione, Oxidative S tress, Valeriana officinalis

Kaynakça

• Abourashed, E.A., Koetter, U., Brattstrom, A. 2004. In vitro binding experiments with a valerian, hops and their fixed combination extract (Ze91019) to selected central nervous system receptors. Phytomedicine, 11: 633-8.
• Andreassen, O.A., Jorgensen, H.A. 2000. Neurotoxicity Associated with Neuroleptic Induced Oral Dyskinesias in rats. Implications for Tardive Dyskinesia? Progress in Neurobiology, 61: 525-41.
• Andreatini, R., Sartori, V.A., Seabra, M.L., Leite, J.R. 2002. Effect of valepotriates (valerian extract) in generalized anxiety disorder: A randomized placebo‐controlled pilot study. Phytotherapy Research, 16: 650‐654.
• Arranz, S., Cert, R., Pérez-Jiménez, J., Cert, A., Saura-Calixto, F. 2008. Comparison between free radical scavenging capacity and oxidative stability of nut oils. Food Chemistry, 110: 985-990.
• Chen, H.W., Wei, B.J., He, X.H., Liu, Y., Wang, J. 2015. Chemical Components and cardiovascular activities of Valeriana spp. Evidence-Based Complementary and Alternative Medicine, doi:10.1155/2015/947619.
• Circosta, C., De Pasquale, R., Samperi, S., Pino, A., Occhiuto, F. 2007. Biological and analytical characterization of two extracts from Valeriana officinalis. Journal of Ethnopharmacology, 112: 361‐367.
• Gülçin, I., Oktay, M., Küfrevioglu, Ö.I., Aslan, A. 2002. Determination of Antioxidant Activity of Lichen Cetraria islandica (L). Journal of Ethnopharmacology, 79: 325-329.
• Hadley, S., Petry, J.J. 2003. Valerian. American Family Physician, 67: 1755‐1758.
• Halliwell, B. ve Gutteridge, J.M., 1990. Role of free radicals and catalytic metal ions in human disease: An overview. Methods in Enzimology, 186: 1-85.
• Jugran, A.K., Bahukhandi, A., Dhyani, P., Bhatt, I.D., Rawal, R.S., Nandi, S.K., Palni, L.M.S. 2015. The effect of inoculation with mycorrhiza: AM on growth, phenolics, tannins, phenolic composition and antioxidant activity in Valeriana jatamansi Jones. Journal of Soil Science and Plant Nutrition, 15(4): 1035-1049.
• Letchamo, W., Ward, W., Heard, B., Heard, D. 2004. Essential oil of Valeriana officinalis L. Cultivars and their antimicrobial activity as influenced by harvesting time under commercial organic cultivation. Journal of Agricultural Food Chemistry, 52 (12): 3915-3919.
• Lister, E., Wilson, P. 2001. Measurement of Total Phenolics and ABTS Assay for Antioxidant Activity (personel communication). Crop Research Institute Lincoln, New Zealand.
• Liu, X.G., Gao, P.Y., Wang, G.S., Song, S.J., Li, L.Z., Li, X., Yao, X.S., Zhang, Z.X. 2012. In vivo antidepressant activity of sesquiterpenes from the roots of Valeriana fauriei Briq. Fitoterapia, 83: 599 603.
• Malva, J.O., Santos, S., Macedo, T. 2004. Neuroprotective properties of Valeriana officinalis extracts. Neurotoxicolgical Research, 6: 131‐40.
• Müller, L.G., Salles, L.A., Stein, A.C., Betti, A.H., Sakamoto, S., Cassel, E., Vargas, R.F., Von Poser, G.L., Rates, S.M.K. 2012. Antidepressant‐like effect of Valeriana glechomifolia Meyer (valerianaceae) in mice. Progress on Neuro-Psychopharmacology & Biological Psychiatry, 36: 101‐109.
• Neamati, A., Chaman, F., Hosseini, M., Boskabady, M.H. 2014. The effects of Valeriana officinalis L. hydro-alcoholic extract on depression like behavior in ovalbumin sensitized rats. Journal of Pharmacy and Bioallied Sciences, 6(2): 97-103.
• Oliveira, D.M., Barreto, G., De Andrade, D.E.V., Saraceno, E., Aon-Bertolino, L., Capani, F., El Bacha, R.S., Giraldez, L.D. 2009. Cytoprotective effect of Valeriana officinalis extract on in vitro experimental model of Parkinson disease. Neurochemical Research, 34: 215-220.
• Pereira, R.P., Fachinetto, R., Prestes, A.S., Puntel, R.L., Silva, G.N.S., Heinzmann, B.M., Boschetti, T.K., Athayde, M.L., Burger, M.E., Morel, A.F., Morsh, V.M., Rocha, J.B.T. 2009. Antioxidant effects of different extracts from Melissa officinalis, Matricaria recutita and Cymbopogon citrates. Neurochemical Research, 34: 973-983.
• Sedlak, J., Lindsay, R.H. 1968. Estimation of Total protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Analytical Biochemistry, 25(1): 192-205.
• Sudati, J.H., Fachinetto, R., Pereira R.P., Boligon, A.A., Athayde, M.L., Soares, F.A., De Vargas Barbosa, N.B., Teixeira Rocha J.B. 2009. In vitro antioxidant activity of Valeriana officinalis against different neurotoxic agents. Neurochemical Research, 34: 1372‐1379.
• Sung, M.N., Jung, H.C., Dae, Y.Y., Woosuk, K., Hyo, Y.J., Jong, W., Soo-Yong, K., Jaeil, P., Dong-Woo, K., Wan, J.K., Yeo, S.Y., In, K.H. 2013. Valeriana officinalis extract and its main component, valerenic acid, ameliorate d-galactose-induced reductions in memory, cell proliferation, and neuroblast differentiation by reducing corticosterone levels and lipid peroxidation. Experimental Gerontology, 48(11): 1369-1377.
• Wu, J.W., Hsieh, C.L., Wang, H.Y., Chen, H.Y. 2009. Inhibitory effects of guava (Psidium guajava L.) leaf extracts and its active compounds on the glycation process of protein. Food Chemistry, 113: 78-84.