Effects of Some Plant Growth Regulators and Phloroglucinol on Micropropagation of SP-2 (Prunus spinosa) Clonal Rootstock

Year 2020, Volume: 7 Issue: 4, 1057 - 1064, 20.10.2020

Esra Bulunuz Palaz

https://doi.org/10.30910/turkjans.678171

Cited By: 1

Abstract

Bu çalışma, 2017 yılında bazı oksin, sitokinin ve phloroglucinol konsantrasyonlarının in vitro kültür koşullarında SP-2(Prunus spinosa) klon anacının rejenerasyon ve köklenme olasılıkları üzerine etkilerini araştırmak amacıyla yürütülmüştür. Materyal olarak ½ MS besin ortamı ve farklı IBA kombinasyonları (0.1-0.5-1.00 mg L-1), NAA (0.25 mg L-1), BA (0.5-1.00-2.00 mg L-1), Kinetin (0.5-1.00-2.00 mg L-1), Phloroglucinol (30-60-90-120 mg L-1) ve MSK-0 (herhangi bir hormon içermeyen normal ½ MS) kullanıldı. Çalışmanın sonunda eksplant başına maksimum sürgün uzunluğunun MSK-7 kombinasyonunda (25.11 mm) olduğu tespit edildi. Ayrıca, en yüksek kök uzunluğu MSK-2 ( 176.74 mm), en yüksek kök sayısı MSK-5 (2 adet/explant) kombinasyonlarında gözlendi. Bu çalışma sonucunda kontrol kombinasyonu (MSK-0) dağılımın alt kısımlarında kalırken, en iyi köklenme değeri MSK-2 ve MSK-3 kombinasyonlarının kullanıldığı bitkilerde görülmüştür. Bu çalışmanın sonucuna göre, Phloroglucinol (PG), klonal anaç adayları SP-2'nin (Prunus spinosa) in vitro çoğaltımında olumlu bir etkiye sahip olduğu belirlenmiştir.

Keywords

In vitro, Prunus spinosa, mikroüretim, erik, anaç

Effects of Some Plant Growth Regulators and Phloroglucinol on Micropropagation of SP-2 (Prunus spinosa) Clonal Rootstock

Abstract

This study was conducted in 2017 in order to work on the impacts of certain auxin, cytokine and phloroglucinol concentrations on propagation and rooting possibilities of SP-2 (Prunus spinosa) in vitro culture conditions. ½ MS nutrient medium and different combinations of IBA (0.1-0.5-1.00 mg L-1), NAA (0.25 mg L-1), BA (0.5-1.00-2.00 mg L-1), Kinetin (0.5-1.00-2.00 mg L-1), Phloroglucinol (30-60-90-120 mg L-1) and MSK-0 (normal ½ MS without any hormone) were used as materials. It was observed at the end of the study that maximum shoot length per explant was at the MSK-7 combination (25.11 mm) and the highest plant multiplication was found as 6 for MSK-10 combinations per explant. In addition, the highest root length was 176.74 mm for the MSK-2 combinations while the highest root number was observed as 2 as per explant for MSK-5 combinations. As a result of this study, while control medium (MSK-0) remained below the distribution, the best rooting value was seen on the material, where MSK-2 and MSK-3 combinations was used. According to the result of this study, Phloroglucinol (PG) had a positive impact in vitro propagation of clonal rootstock candidates SP-2 (P. spinosa).

Keywords

In vitro, micropropagation, plum, Prunus spinosa, rootstock

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