In vitro Carbonic Anhydrase Inhibitory Effects of The Extracts of Satureja cuneifolia

Yıl 2021, Cilt: 8 Sayı: 4, 1146 - 1150, 24.10.2021

Fatma Gülruy Aydın , Emir Alper Türkoğlu , Müslüm Kuzu , Turgut Taşkın

https://doi.org/10.30910/turkjans.980819

Cited By: 1

Öz

Carbonic anhydrases catalyze the interconversion between carbon dioxide and bicarbonate. Carbonic anhydrase inhibition has therapeutic importance and there are many drugs which use this mechanism. Carbonic anhydrase inhibitors are used as diuretic, antiglaucoma, antitumor and antiepileptic agents. As an alternative for synthetic chemicals with strong side effects, natural products have gained popularity in the recent years. In this study, Satureja cuneifolia, a medicinal plant used as folk medicine, was investigated for its inhibition effects on carbonic anhydrase I and II enzymes. Four different extracts were obtained with maceration method and three different solvents were used. The results have shown that methanol extracts of Satureja cuneifolia has the strongest inhibition activity on the enzymes with the IC50 values of 31 µg/mL for hCA I and 12 µg/mL for hCA II. Further purification and analytical studies will be needed to obtain the active natural molecules and their potential for pharma and food industries.

Anahtar Kelimeler

Carbonic anhydrase, Inhibition, Extract, Satureja cuneifolia

Teşekkür

The authors thank Dr. İsmail ŞENKARDEŞ from Department of Pharmaceutical Botany at Marmara University for his help in plant identification.

Satureja cuneifolia Ekstraktlarının in vitro Karbonik Anhidraz İnhibitör Etkileri

Öz

Karbonik anhidrazlar, karbon dioksit ve bikarbonat arasındaki karşılıklı dönüşümü katalize eder. Karbonik anhidraz inhibisyonunun terapötik önemi vardır ve bu mekanizmayı kullanan birçok ilaç vardır. Karbonik anhidraz inhibitörleri diüretik, antiglokom, antitümör ve antiepileptik ajanlar olarak kullanılmaktadır. Güçlü yan etkileri olan sentetik kimyasallara alternatif olarak son yıllarda doğal ürünler popülerlik kazanmıştır. Bu çalışmada, halk ilacı olarak kullanılan tıbbi bir bitki olan Satureja cuneifolia’nın karbonik anhidraz I ve II enzimleri üzerindeki inhibisyon etkileri araştırılmıştır. Maserasyon yöntemi ile dört farklı ekstrakt elde edilmiş ve üç farklı çözücü kullanılmıştır. Sonuçlar, Satureja cuneifolia’nın metanol ekstrelerinin, hCA I için 31 µg/mL ve hCA II için 12 µg/mL IC50 değerleri ile enzimler üzerinde en güçlü inhibisyon aktivitesine sahip olduğunu göstermiştir. Aktif doğal moleküllerin elde edilebilmesi ve bunların ilaç ve gıda endüstrileri için potansiyellerini değerlendirmek için daha fazla saflaştırma ve analitik çalışmalara ihtiyaç duyulmaktadır.

Anahtar Kelimeler

Karbonik anhidraz, İnhibisyon, Ekstrakt, Satureja cuneifolia

Kaynakça

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