Urtica dioica, Silybum marianum ve Cynara scolymus Ekstraktlarının Antikanser, Antibakteriyel ve Antioksidan Aktivitesi

Yıl 2022, , 51 - 62, 28.04.2022

Rabia Yılmaz , Hilal Çalık , Hatice Feyzan Ay , Fatih Erci , Rabia Çakır Koç

https://doi.org/10.54537/tusebdergisi.1089792

Öz

Mide kanseri, dünya çapında en yaygın kanser türlerinden biridir ve kansere bağlı ölümlerin önde gelen nedenlerindendir. Bu kanser türü, moleküler düzeyde yeterince anlaşılamamış bir karsinogeneze sahip, agresif ve heterojen bir hastalıktır. Bu nedenle etkili ilaç tedavi stratejilerine yönelik yapılan araştırmalar, hastalığın tedavisinde önemli bir rol oynamaktadır. Bu etkili tedavi stratejilerinden biri, yan etkileri düşük ve içeriğinde biyolojik olarak aktif birçok bileşik bulunan bitkisel bazlı terapötiklerdir. Bu çalışmada U.dioica, S. marianum ve C. scolymus bitkisel ekstraktlarının antikanser aktivitelerinin değerlendirilmesi için L929, AGS ve SH-SY5Y hücre hatlarındaki hücre canlılığına etkisi XTT testi ile analiz edilmiştir. Ekstraktların antibakteriyel ve antioksidan aktiviteleri için ise sırasıyla agar kuyusu difüzyon testi ve CUPRAC yöntemi kullanılmıştır. U. dioica ve S. marianum ekstraktlarının AGS ve SH-SY5Y kanser hücrelerinin canlılığı üzerinde önemli bir etki göstermediği görülmüştür. C. scolymus ekstraktı ise tüm konsantrasyonlarda AGS kanser hücreleri üzerinde yüksek antikanser etki göstermiş ancak SH-SY5Y hücreleri üzerinde hiçbir etkiye neden olmamıştır. U. dioica ve C. scolymus ekstraktları sırasıyla S. aureus ve B. cereus bakterilerine karşı antibakteriyel aktivite sergilemiştir. S. marianum ekstraktı herhangi bir antibakteriyel etki göstermemiştir. CUPRAC testi sonucunda U. dioica ve S. marianum ekstraktlarının güçlü antioksidan aktivite gösterdiği bulunmuştur. Sonuç olarak, elde edilen sonuçlar enginar olarak bilinen C. scolymus eksraktının antibakteriyel ve mide kanseri hücrelerinde antikanser terapötik potansiyelini ortaya koymuştur. Ancak, bu ekstraktın terapötik özelliklerinin daha iyi anlaşılması için daha fazla araştırmaya ihtiyaç vardır.

Anahtar Kelimeler

Urtica dioica, Silybum marianum, Cynara scolymus, Antikanser aktivite, Antibakteriyel aktivite

Anticancer, Antibacterial and Antioxidant Activity of Urtica dioica, Silybum marianum and Cynara scolymus Extracts

Öz

Gastric cancer (GC) is one of the most prevalent cancer types worldwide and one of the leading causes of cancer-related deaths. Gastric cancer is an aggressive and heterogeneous disease with a poorly understood carcinogenesis at the molecular level. Therefore, the research for effective drug therapy strategies plays a significant role in treating the disease. One of these effective treatment strategies is herbal-based therapeutics, which have low side effects and contain many biologically active compounds. In this study, the effect of U.dioica, S. marianum and C. scolymus herbal extracts on cell viability in L929, AGS and SH-SY5Y cell lines was analyzed by XTT test to evaluate the anticancer activities. Antibacterial and antioxidant activities of extracts were determined by the agar well diffusion test and CUPRAC method, respectively. We found that U. dioica and S. marianum extracts showed no significant effect on the viability of AGS and SH-SY5Y cancer cells. C. scolymus extract demonstrated strong anticancer activity on AGS cancer cells at all concentrations but had no effect on SH-SY5Y cells. U. dioica and C. scolymus exhibited antibacterial activity against S. aureus and B. cereus, respectively. No antibacterial activity was found in S. marianum extract. U. dioica and S. marianum extracts have shown strong antioxidant activity in CUPRAC assay. In conclusion, the obtained results revealed the antibacterial and anticancer therapeutic potential of C. scolymus extract known as artichoke in gastric cancer cells. However, more research is required to better explain the therapeutic properties of these extracts.

Anahtar Kelimeler

Urtica dioica, Silybum marianum, Cynara scolymus, Anticancer activity, Antibacterial activity

Kaynakça

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