Effect of Drought Stress on Germination and Seedling Growth of Common Vetch (Vicia sativa L.) Cultivars

Öz

This study aimed to determine the germination and seedling development responses of common vetch (Vicia sativa L.) varieties under drought stress and identify sensitive/tolerant varieties. The study was conducted at the Laboratory of the Department of Field Crops, Faculty of Agriculture, Siirt University. Artificial drought stress was induced using polyethylene glycol (PEG-6000) material. The plant material for the study consisted of the following common vetch varieties: Görkem, Kralkızı, Özveren, Dicle, Alper, Doruk, Kubilay-82, D-135, IFVS-715, and Alınoğlu-2001. These varieties were germinated under different drought (PEG-6000) levels (0%, 5%, 10%, 15%, and 20%). The laboratory experiment was conducted with a randomized split-plot design, with four replications. In the research, parameters such as germination percentage, mean germination time, germination index, germination uniformity coefficient, radicle and hypocotyl length, lateral root number and length, seedling fresh and dry weights, and seedling vigor index were examined. Increasing drought levels significantly (p<0.01) affected germination and seedling characteristics of common vetch varieties. The effects of drought stress were observed from the 10% PEG level for germination percentage and from the 5% PEG concentration for all other parameters. According to the research findings, the tolerance of varieties to drought levels also varied. Among the common vetch varieties, Dicle, Alınoğlu-2001, and Kralkızı showed higher tolerance to drought in terms of germination and seedling development parameters compared to other varieties. Considering genotypic differences, the use of drought-resistant varieties is important in common vetch cultivation in drought-affected areas.

Anahtar Kelimeler

• Common vetch
• drought stress
• germination percentage
• germination index
• seedling vigor index

Kuraklık Stresinin Yaygın Fiğ (Vicia sativa L.) Çeşitlerinin Çimlenme ve Fide Gelişimine Etkisi

Öz

Bu çalışmada, yaygın fiğ (Vicia sativa L.) çeşitlerinin kuraklık stresi altında çimlenme ve fide gelişim tepkilerinin belirlenmesi ve hassas/tolerant çeşitlerin ortaya konulması amaçlanmıştır. Çalışma; Siirt Üniversitesi, Ziraat Fakültesi, Tarla Bitkileri Bölümü Laboratuvarı’nda yürütülmüştür. Yapay kuraklık stresi, polietilen glikol (polyethylene glycol, PEG-6000) materyali ile sağlanmıştır. Çalışmanın bitkisel materyalini yaygın fiğin Görkem, Kralkızı, Özveren, Dicle, Alper, Doruk, Kubilay-82, D-135, IFVS-715 ve Alınoğlu-2001 çeşitleri oluşturmuştur. Bu çeşitler, farklı kuraklık (PEG-6000) seviyeleri (% 0, % 5, % 10, % 15 ve % 20) altında çimlendirilmiştir. Laboratuvar deneyi tesadüf parsellerinde bölünmüş parseller deneme desenine göre 4 tekrarlamalı olarak yürütülmüştür. Araştırmada; çimlenme yüzdesi, ortalama çimlenme süresi, çimlenme indeksi, çimlenme üniformite katsayısı, kökçük ve sapçık uzunluğu, lateral kök sayısı ve uzunluğu, fide yaş ve kuru ağırlıkları ile fide güç indeksi parametreleri incelenmiştir. Kuraklık seviyesindeki artış, yaygın fiğ çeşitlerinde çimlenme ve fide özelliklerini çok önemli (p<0.01) derecede etkilemiştir. Kuraklık stresinin etkileri çimlenme yüzdesinde % 10 PEG seviyesinde, diğer tüm parametrelerde % 5 PEG konsantrasyonundan itibaren gözlemlenmiştir. Araştırma sonucuna göre kuraklık seviyelerine karşı çeşitlerin gösterdiği stres toleransı da farklılık göstermiştir. Yaygın fiğ çeşitlerinden Dicle, Alınoğlu-2001 ve Kralkızı çeşitleri, çimlenme ve fide gelişim parametreleri açısından kuraklığa diğer çeşitlere göre daha toleranslı olmuştur. Kuraklık yaşanan alanlarda, yaygın fiğ yetiştiriciliğinde genotipik farklılık dikkate alınarak kuraklığa dayanıklı çeşitlerin kullanılması önem taşımaktadır.

Anahtar Kelimeler

• Yaygın fiğ
• kuraklık stresi
• çimlenme yüzdesi
• çimlenme indeksi
• fide güç indeksi

Kaynakça

1. Acikbas, S., Ozyazici, M.A., Bektas, H., 2021. The effect of salinity on root architecture in forage pea (Pisum sativum ssp. arvense L.). Legume Research-An International Journal, 44(4): 407-412.
2. Açıkgöz, N., Açıkgöz, N., 2001. Tarımsal araştırmaların istatistiki değerlendirilmesinde yapılan bazı hatalar: I. Tek faktörlü denemeler. Anadolu, 11(1): 135-147.
3. Ahmadloo, F., Tabari, M., Behtari, B., 2011. Effect of drought stress on the germination parameters of Cupressus seeds. International Journal of Forest, Soil and Erosion, 1(1): 11-17.
4. Álvarez-Aragón, R., Palacios, J.M., Ramírez-Parra, E., 2023. Rhizobial symbiosis promotes drought tolerance in Vicia sativa and Pisum sativum. Environmental and Experimental Botany, 208: 105268.
5. Anonymous, 2019. Summary for policymakers. In: P.R. Shukla, J. Skea, E. Calvo Buendia, V. Masson-Delmotte, H.-O. Pörtner, D.C. Roberts, P. Zhai, R. Slade, S. Connors, R. van Diemen, M. Ferrat, E. Haughey, S. Luz, S. Neogi, M. Pathak, J. Petzold, J. Portugal Pereira, P. Vyas, E. Huntley, K. Kissick, M. Belkacemi, J. Malley (Eds.), Climate Change and Land: An IPCC Special Report on Climate Change, Desertification, Land Degradation, Sustainable Land Management, Food Security, and Greenhouse Gas Fluxes in Terrestrial Ecosystems. (https://www.ipcc.ch/site/assets/uploads/sites/4/2022/11/SRCCL_SPM.pdf), (Erişim Tarihi: 14.06.2023).
6. Arslan, M., Aksu, E., Doğan, E., 2018. Kuraklık stresine tolerans bakımından iki mürdümük (Latyhrus sativus L.) genotipinin değerlendirilmesi. Türk Tarım ve Doğa Bilimleri Dergisi, 5(3): 261-267.
7. Aslan, H., Atış, İ., 2018. Bazı yaygın mürdümük çeşitlerinde kuraklık stresinin çimlenme ve fide gelişimine etkisi. Mustafa Kemal Üniversitesi Ziraat Fakültesi Dergisi, 23(2): 218-231.
8. Asma, H.I., Ashraf, M.Y., Saleem, M.H., Ashraf, M.A., Ali, B., Shereen, A., Farid, G., Ali, M., Shirazi, M.U., Yasin, G., 2023. Alleviating effects of salicylic acid spray on stage-based growth and antioxidative defense system in two drought-stressed rice (Oryza sativa L.) cultivars. Turkish Journal of Agriculture and Forestry, 47(1): 79-99.

9. Aydın, M., Hossein Pour, A., Haliloğlu, K., Tosun, M., 2015. Effect of putrescine application and drought stress on germination of wheat (Triticum aestivum L.). Atatürk University Journal of the Agricultural Faculty, 46(1): 43-45.
10. Babu, K., Rosaiah, G., 2017. A study on germination and seedling growth of Blackgram (Vigna mungo L. Hepper) germplasm against Polyethylene glycol 6000 stress. IOSR Journal of Pharmacy and Biological Sciences, 12(5): 90-98.
11. Bewely, J., Black, M., 1994. Seeds: Physiology of Development and Germination. New York.
12. Bilgili, D., Atak M., Mavi, K., 2018. Bazı ekmeklik buğday genotiplerinde tuz ve kuraklık stresinin çimlenme ve fide gelişimine etkisi. Mustafa Kemal Üniversitesi Ziraat Fakültesi Dergisi, 23(1): 85-96.
13. Bilir Ekbic, H., Gecene, İ., Ekbic, E., 2022. Determination of the tolerance of fox grapes (Vitis labrusca L.) to drought stress by PEG application in vitro. Erwerbs-Obstbau, 64(1): 87-94.
14. Borawska-Jarmułowicz, B., Mastalerczuk, G., Gozdowski, D., Małuszyńska, E., Szydłowska, A., 2017. The sensitivity of Lolium perenne and Poa pratensis to salinity and drought during the seed germination and under different photoperiod conditions. Zemdirbyste-Agriculture, 104(1): 71-78.
15. Caruso, A., Chefdor, F., Carpin, S., Depierreux, C., Delmotte, F.M., Kahlem, G., Morabito, D. 2008. Physiological characterization and identification of genes differentially expressed in response to drought induced by PEG 6000 in Populus canadensis leaves. Journal of Plant Physiology, 165(9): 932-41.
16. Chai, X., Dong, R., Liu, W., Wang, Y., Liu, Z., 2017. Optimizing sample size to assess the genetic diversity in common vetch (Vicia sativa L.) populations using start codon targeted (SCoT) markers. Molecules, 22(4): 567.
17. Chen, J., Wu, W., Zheng, Y., Hou, K., Xu, Y., Zai, J., 2010. Drought resistance of Angelica dahurica during seedling stage under polyethylene glycol (PEG-6000)-simulated drought stress. China journal of Chinese Materia Medica, 35(2): 149-153.
18. Çarpıcı, B.E., Erdel, B., 2015. Bazı yonca çeşitlerinde (Medicago sativa L.) kuraklık stresinin çimlenme özellikleri üzerine etkisi. Derim, 32(2): 201-210.
19. Çığ, F., Erman, M., İnal, B., Bektaş, H., Sonkurt, M., Mirzapour, M., Ceritoğlu, M., 2022. Mitigation of drought stress in wheat by bio-priming by PGPB containing ACC deaminase activity. Ataturk University Journal of Agricultural Faculty, 53(1): 51-57.
20. Daryanto, S., Wang, L., Jacinthe, P.A., 2015. Global synthesis of drought effects on food legume production. PloS One, 10(6): e0127401.
21. De la Rosa, L., Zambrana, E., Ramirez-Parra, E., 2020. Molecular bases for drought tolerance in common vetch: designing new molecular breeding tools. BMC Plant Biology, 20(1): 1-18.
22. Dezfuli, P.M., Sharif-zadeh, F., Janmohammadi, M., 2008. Influence of priming techniques on seed germination behavior of maize inbred lines (Zea mays L.). ARPN Journal of Agricultural and Biological Science, 3(3): 22-25.
23. Dola, D.B., Mannan, M.A., Sarker, U., Mamun, M.A.A., Islam, T., Ercisli, S., Saleem, M.H., Ali, B., Pop, O.L., Marc, R.A., 2022. Nano-iron oxide accelerates growth, yield, and quality of Glycine max seed in water deficits. Frontiers in Plant Science, 13: 992535.
24. Ellis, R.A., Roberts, E.H., 1981. The quantification of ageing and survival in orthodox seed. Seed Science and Technology, 9(2): 373-409.
25. Fallahi, H.R., Fadaeian, G., Gholami, M., Daneshkhah, O., Hosseini, F.S., Aghhavani-Shajari, M., Samadzadeh, A., 2015. Germination response of grasspea (Lathyrus sativus L.) and arugula (Eruca sativa L.) to osmotic and salinity stresses. Plant Breeding and Seed Science, 71(1): 97-108.
26. Fang, X., Turner, N.C., Yan, G., Li, F., Siddique, K.H.M., 2010. Flower numbers, pod production, pollen viability, and pistil function are reduced and flower and pod abortion increased in chickpea (Cicer arietinum L.) under terminal drought. Journal of Experimental Botany, 61(2): 335-345.
27. Farooq, M., Gogoi, N., Barthakur, S., Baroowa, B., Bharadwaj, N., Alghamdi, S.S., 2016. Drought stress in grain legumes during reproduction and grain filling. Journal of Agronomy and Crop Science, 203(2): 81-102.
28. Farooq, M., Wahid, A., Kobayashi, N., Fujita, D., Basra, S.M.A., 2009. Plant Drought Stress: Effects, Mechanisms and Management. In: Lichtfouse, E., Navarrete, M., Debaeke, P., Véronique, S., Alberola, C. (eds) Sustainable Agriculture. Springer, Dordrecht.
29. Farooq, T.H., Rafy, M., Basit, H., Shakoor, A., Shabbir, R., Riaz, M.U., Ali, B., Kumar, U., Qureshi, K.A., Jaremko, M., 2022. Morpho-physiological growth performance and phytoremediation capabilities of selected xerophyte grass species towards Cr and Pb stress. Frontiers in Plant Science, 13: 997120.
30. Farsiani, A., Ghobadi, M.E., 2009. Effects of PEG and NaCl stress on two cultivars of corn (Zea mays L.) at germination and early seedling stages. International Journal of Agricultural and Biosystems Engineering, 3(9): 442-445.
31. Feng, J., Wang, D., Shao, C., Zhang, L., Tang, X., 2018. Effects of cold plasma treatment on alfalfa seed growth under simulated drought stress. Plasma Science and Technology, 20(3): 35-50.
32. Gheidary, S., Akhzari, D., Pessarakli, M., 2017. Effects of salinity, drought, and priming treatments on seed germination and growth parameters of Lathyrus sativus L. Journal of Plant Nutrition, 40(10): 1-23.
33. Ghorbanpour, A., Mami, Y., Ashournezhad, M., Abri, F., Amani, M., 2011. Effect of salinity and drought stress on germination of fenugreek. African Journal of Agricultural Research, 6(24): 5529-5532.
34. Guo, S.J., Yang, K.M., Huo, J., Zhou, Y.H., Wang, Y.P., Li, G.Q., 2015. Influence of drought on leaf photosynthetic capacity and root growth of soybeans at grain filling stage. Ying Yong Sheng Tai Xue Bao= Chinese Journal of Applied Ecology, 26(5): 1419-1425.
35. Gürbüz, A., Kaya, M., Divanlı Türkan, A., Kaya, G., Kaya, M.D., Çiftçi, C.Y., 2009. Bazı nohut (Cicer arietinum L.) çeşitlerinde tane iriliği ve kuraklık stresinin çimlenme özelliklerine etkisi. Akdeniz Üniversitesi Ziraat Fakültesi Dergisi, 22(1): 69-74.
36. Huang, Y.F., Gao, X.L., Nan, Z.B., Zhang, Z.X., 2017. Potential value of the common vetch (Vicia sativa L.) as an animal feedstuff: a review. Journal of Animal Physiology and Animal Nutrition, 101(5): 807-823.
37. Huang, Y. Zhang, Z., Nan, Z., Unkovich, M., Coulter, J.A., 2021. Effects of cultivar and growing degree day accumulations on forage partitioning and nutritive value of common vetch (Vicia sativa L.) on the Tibetan plateau. Journal of the Science of Food and Agriculture, 101(9): 3749-3757.
38. Kalsa, K.K., Abebie, B., 2012. Influence of seed priming on seed germination and vigor traits of Vicia villosa ssp. dasycarpa (Ten.). African Journal of Agricultural Research, 7(21): 3202-3208.
39. Khan, M.A., Kashmir, S., Ali, H.H., Gul, B., Raza, A., Umm-E-Kulsoom, U., Uslu, O.S., Waheed, H., 2019. Environmental factors can affect the germination and growth of Parthenium hysterophorus and Rumex crispus. Pakistan Journal of Botany, 51(6): 2195-2202.
40. Khodarhmpour, Z., 2011. Effect of drought stress induced by polyethylene glycol (PEG) on germination indices in corn (Zea mays L.) hybrids. African Journal of Biotechnology, 10(79): 18222-18227.
41. Kumar, N., Nandwal, A.S., Devi, S., Sharma, K.D., Yadav, A., Waldia, R.S., 2010. Root characteristics, plant water status and CO2 exchange in relation to drought tolerance in chickpea. Journal of SAT Agricultural Research, 8: 1-5.
42. Levitt, J., 1980. Responses of Plants to Environmental Stress. Academic Press, New York.
43. Li, Y., Li, H., Li, Y., Zhang, S., 2017. Improving water-use efficiency by decreasing stomatal conductance and transpiration rate to maintain higher ear photosynthetic rate in drought-resistant wheat. The Crop Journal, 5(3): 231-239.
44. Liu, Z., Liu, P., Luo, D., Liu, W., Wang, Y., 2014. Exploiting Illumina sequencing for the development of 95 novel polymorphic EST-SSR markers in common vetch (Vicia sativa subsp. sativa). Molecules, 19(5): 5777-5789.
45. López, R., Aranda, I., Gil, L., 2009. Osmotic adjustment is a significant mechanism of drought resistance in Pinus pinaster and Pinus canariensis. Investigación Agraria: Sistemas y Recursos Forestales, 18(2): 159-166.
46. Micheletto, S., Rodriguez-uribe, L., Hernandez, R., Richins, R.D., Curry, J., Connell, M.A.O., 2007. Comparative transcript profiling in roots of Phaseolus acutifolius and P. vulgaris under water deficit stress. Plant Sciences, 173(5): 510-520.
47. Molor, A., Khajidsuren, A., Myagmarjav, U., Vanjidorj, E., 2016. Comparative analysis of drought tolerance of Medicago L. plants under stressed conditions. Mongolian of Agricultural Sciences, 19(03): 32-40.
48. Mujtaba, S.M., Faisal, S., Khan, M.A., Mumtaz, S., Khanzada, B., 2016. Physiological studies on six wheat (Triticum aestivum L.) genotypes for drought stress tolerance at seedling stage. Agricultural Research & Technology: Open Access Journal, 1(2): 1-6.
49. Muscolo, A., Sidari, M., Anastasi, U., Santonoceto, C., Maggio, A., 2014. Effect of PEG-induced drought stress on seed germination of four lentil genotypes. Journal of Plant Interactions, 9(1): 354-363.
50. Mut, Z., Akay, H., 2010. Effect of seed size and drought stress on germination and seedling growth of naked oat (Avena sativa L.). Bulgarian Journal of Agricultural Science, 16(4): 459-467.
51. Nguyen, V., Riley, S., Nagel, S., Fisk, I., Searle, I.R., 2020. Common vetch: a drought tolerant, high protein neglected leguminous crop with potential as a sustainable food source. Frontiers in Plant Science, 11: 818.
52. Niu, J., Zhang, S., Liu, S., Ma, H., Chen, J., Shen, Q., Ge, C., Zhang, X., Pang, C., Zhao, X., 2018. The compensation effects of physiology and yield in cotton after drought stress. Journal of Plant Physiology, 224-225: 30-48.
53. Okçu, G., Kaya, M.D., Atak, M., 2005. Effects of salt and drought stresses on germination and seedling growth of pea (Pisum sativum L.). Turkish Journal of Agriculture and Forestry, 29(4): 237-242.
54. Özyazıcı, M.A., 2022. Yeşil Gübreleme ve Yeşil Gübreleme Amacıyla Kullanılan Bitkiler. In: M.A. Özyazıcı ve S. Açıkbaş (Editörler), Yeşil Gübreleme, Iksad Publishing House, Ankara, Türkiye, s. 3-50.
55. Özyazıcı, M.A., Manga, İ., 2000. Çarşamba ovası sulu koşullarında yeşil gübre olarak kullanılan bazı baklagil yembitkileri ile bitki artıklarının kendilerini izleyen mısır ve ayçiçeğinin verim ve kalitesine etkileri. Türk Tarım ve Ormancılık Dergisi, 24(1): 95-103.
56. Pandey, J., Devadasu, E., Saini, D., Dhokne, K., Marriboina, S., Raghavendra, A.S., Subramanyam, R., 2023. Reversible changes in structure and function of photosynthetic apparatus of pea (Pisum sativum) leaves under drought stress. The Plant Journal, 113(1): 60-74.
57. Pantola, S., Vibhuti Bargali, K., Bargali, S.S., 2017. Screening of three leguminous crops for drought stress tolerance at germination and seedling growth stage. Indian Journal of Agricultural Sciences, 87(4): 467-472.
58. Partheeban, C., Chandrasekhar, C.N., Jeyakumar, P., Ravikesavan, R., Gnanam, R., 2017. Effect of PEG induced drought stress on seed germination and seedling characters of maize (Zea mays L.) genotypes. International Journal of Current Microbiology and Applied Sciences, 6(5): 1095-1104.
59. Pawar, K.N., Veena, V.B., 2020. Evaluation of cotton genotypes for drought tolerance using PEG-6000 water stress by slanting glass plate technique. International Journal of Current Microbiology and Applied Sciences, 9(11): 3203-3212.
60. Petrovic, G., Jovicic, D., Nikolic, Z., Tamindzic, G., Ignjatov, M., Milosevic, D., Milosevic, B., 2016. Comparatıve study of drought and salt stress effects on germınatıon and seedling growth of pea. Genetika, 48(1): 373-381.
61. Piwowarczyk, B., Kaminska, I., Rybinski, W., 2014. Influence of PEG generated osmotic stress on shoot regeneration and some biochemical parameters in Lathyrus culture. Czech Journal Genetic Plant Breed, 50(2): 77-83.
62. Quan, W., Liu, X., Wang, H., Chan, Z. 2016. Comparative physiological and transcriptional analyses of two contrasting drought tolerant alfalfa varieties. Frontiers in Plant Science, 6: 1256.
63. Rouhi, V., Samson, R., Lemeur, R., Van Damme, P. 2006. Stomatal resistance under drought stress conditions induced by PEG 6000 on wild almond. Communications in Agricultural and Applied Biological Sciences, 71(1): 269-273.
64. Rueden, C.T., Schindelin, J., Hiner, M.C., DeZonia, B.E., Walter, A.E., Arena, E.T., Eliceiri, K.W., 2017. ImageJ2: Imagej for the next generation of scientific image data. BMC Bioinformatics, 18(1): 529.
65. Ruffini Castiglione, M., Frediani, M., Gelati, M. T., Ravalli, C., Venora, G., Caputo, P., Cremonini, R., 2011. Cytology of Vicia species. X. Karyotype evolution and phylogenetic implication in Vicia species of the sections Atossa, Microcarinae, Wiggersia and Vicia. Protoplasma, 248(4): 707-716.
66. Sabagh, A.E.L., Hossain, A., Islam, M.S., Iqbal, M.A., Amanet, K., Mubeen, M., Nasim, W., Wasaya, A., Llanes, A., Ratnasekera, D., Singhal, R.K., Kumari, A., Meena, R.S., Abdelhamid, M., Hasanuzzaman, M., Raza, M.A., Özyazici, G., Ozyazici, M.A., Erman, M., 2021. Prospective role of plant growth regulators for tolerance to abiotic stresses. In: T. Aftab and K.R. Hakeem (Eds.), Plant Growth Regulators, 1st Eds., Springer, Cham., Switzerland, pp. 1-38.
67. Scott, S.J., Jones, R.A., Williams, W.A., 1984. Review of data analysis methods for seed germination. Crop Science, 24(6): 1192-1199.
68. Seleiman, M.F., Al-Suhaibani, N., Ali, N., Akmal, M., Alotaibi, M., Refay, Y., Battaglia, M.L., 2021. Drought stress impacts on plants and different approaches to alleviate its adverse effects. Plants, 10(2): 259.
69. Soleymani, A., Shahrajabian, M.H., 2018. Changes in germination and seedling growth of different cultivars of cumin to drought stress. Cercetări Agronomice în Moldova, 1(173): 91-100.
70. Şahin, H.H., 2019. Türkiye’de yetiştirilen bazı yem bezelyesi (Pisum sativum spp. arvense L.) çeşitlerinin kuraklık stresine tepkilerinin belirlenmesi. Yüksek Lisans Tezi, Akdeniz Üniversitesi, Fen Bilimleri Enstitüsü, Antalya, 32s.
71. Tiryaki, İ., 2016. Yoncada (Medicago sativa L.) kuraklık stresi ve tolerantlık mekanizması. Kahramanmaraş Sütçü İmam Üniversitesi Doğa Bilimleri Dergisi, 19(3): 296-304.
72. Toosi, A.F., Bakar, B., Azizi, M., 2014. Effect of drought stress by using PEG 6000 on germination and early seedling growth of Brassica juncea var. Ensabi. Scientific Papers. Series A. Agronomy, Vol. LVII, 360-363.
73. Türkan, I., Bor, M., Özdemir, F., Koca, H., 2005. Differential responses of lipid peroxidants in the leaves of drought tolerant P. acutifolius gray and drought sensitive P. vulgaris L. subjected to polyethylene glycol mediated water stress. Plant Science, 168(1): 223-231.
74. Uslu, Ö. S., Gedik, O., Alhumedi, M., Alminfi, K., 2021. Kuraklık stresinin bazı yem bezelyesi (Pisum sativum L.) çeşitlerinin çimlenme ve fide gelişimi üzerine etkisi. Uluslararası Anadolu Ziraat Mühendisliği Bilimleri Dergisi, 3(2): 28-36.
75. Wang, W., Wang, C., Pan, D., Zhang, Y., Luo, B., Ji, J., 2018. Effects of drought stress on photosynthesis and chlorophyll fluorescence images of soybean (Glycine max) seedlings. International Journal of Agricultural and Biological Engineering, 11(2): 196-201.
76. Wang, Y.R., Yu, L., Nan, Z.B., Liu, Y.L., 2004. Vigor tests used to rank seed lot quality and predict field emergence in four forage species. Crop Sciences, 44(2): 535-541.
77. Wei, Y., Jin, J., Jiang, S., Ning, S., Liu, L., 2018. Quantitative response of soybean development and yield to drought stress during different growth stages in the Huaibei Plain China. Agronomy, 8(7): 97.
78. Yasmeen, S., Wahab, A., Saleem, M.H., Ali, B., Qureshi, K.A., Jaremko, M., 2022. Melatonin as a foliar application and adaptation in lentil (Lens culinaris Medik.) crops under drought stress. Sustainability, 14: 16345.
79. Yılmaz, M.B., Kısakürek, Ş., 2020. Tuz ve PEG kaynaklı kuraklık stresinin çok yıllık çim (Lolium perenne L.) çeşitlerinde fide gelişimine etkisi. Mustafa Kemal Üniversitesi Tarım Bilimleri Dergisi, 25(3): 360-369.
80. Yılmaz, M., Doğru, A., Kozan, Y., 2022. Kuraklık stresinin bazı serin iklim çim alan buğdaygillerinin çimlenmesi ve sürgün gelişimi üzerine etkileri. Journal of Agricultural Biotechnology, 3(1): 1-10.
81. Yousefi, A.R., Rashidi, S., Moradi, P., Mastinu, A., 2020. Germination and seedling growth responses of Zygophyllum fabago, Salsola kali L. and Atriplex canescens to PEG-induced drought stress. Environments, 7(107): 1-10.
82. Zar, J.H., 1996. Biostatistical Analysis. 3rd Edition, Prentice Hall, New Jersey, U.S.A.