Investigation of Fungal Causal Agents Root Rot and Wilt on Pistachio (Pistacia vera L.) Orchard: Siirt Province Location, Türkiye

Öz

In recent years, symptoms such as root rot and wilt have been observed in pistachio (Pistacia vera L.) orchards in this province, and drying of trees has been detected. This study investigated soil-borne fungal pathogens that cause diseases in crops. Survey studies were carried out according to the controlled sampling survey method in Siirt province center, Eruh, Şirvan, Kurtalan districts and villages in 2021-2022. Fungal species were determined based on morphological characters such as color, growth in nutrient media, and spore structures, and by matching analysis of Internal Transcribed Spacer (ITS) gene sequences with other genes in the National Center for Biotechnology Information (NCBI) gene bank. Pathogenicity study was carried out on one-year-old Siirt variety in the greenhouse and pathogenic isolates were determined. As a result of the study, mostly Fusarium species (F. solani, F. oxysporum, F. verticillioides, F. equseti, F. acuminatum, F. avenaceaum, F. proliferatum, F. brchygibbosum, Fusarium spp.) and Diaporthe hongkongensis, Scydalidium sp., Rosellinia sp., Nectria diminuta, Neoscytalidium dimitatum, Rhizoctonia solani, Macrophomina phaseolina, Phoma spp., and Cylindrocarpon spp. were isolated and some of them found to be pathogenic at different rates.

Anahtar Kelimeler

• Pistachio
• soil pathogens
• polymerase chain reaction
• Siirt

Antepfıstığı (Pistacia vera L.) Bahçelerinde Kök Çürüklüğü ve Solgunluğa Neden Olan Fungal Etmenlerin Araştırılması: Siirt İli Lokasyonu, Türkiye

Öz

Antepfıstığı (Pistacia vera L.) bahçelerinde son yıllarda kök çürüklüğü ve solgunluk gibi belirtiler gözlemlenmiş ve ağaçlarda kurumalar tespit edilmiştir. Bu çalışmada kurumalara neden olan toprak kökenli fungal patojenler araştırılmıştır. Çalışma; Siirt ili Merkez, Eruh, Şirvan, Kurtalan ilçeleri ve köylerinde 2021-2022 yıllarında güdümlü örnekleme sörvey metoduna göre yürütülmüştür. Elde edilen fungus türleri, renk, gelişim durumları, spor yapıları gibi morfolojik karakterlerine ve ITS (Internal Transcribed Spacer) gen dizilerinin NCBI (The National Center for Biotechnology Information) gen bankasındaki diğer genlerle eşleştirme analizleri ile belirlenmiştir. Patojenisite çalışması, bir yaşındaki Siirt çeşidi üzerinde yapılmış ve patojen izolatlar belirlenmiştir. Çalışma sonucunda ağırlıklı olarak Fusarium türleri (F. solani, F. oxysporum, F. verticillioides, F. equseti, F. acuminatum, F. avenaceaum, F. proliferatum, F. brchygibbosum, Fusarium spp.) ile Diaporthe hongkongensis, Scydalidium sp., Rosellinia sp., Nectria diminuta, Neoscytalidium dimitatum, Rhizoctonia solani, Macrophomina phaseolina, Phoma spp. ve Cylindrocarpon spp., izole edilmiş ve bunların farklı oranlarda patojen oldukları tespit edilmiştir.

Anahtar Kelimeler

• Antepfıstığı
• toprak patojenleri
• polimeraz zincir reaksiyonu
• Siirt

Kaynakça

1. Agrios, G.N., 2005. Plant Pathology (5th Edition). Elsevier-Academic Press, San Diego, CA.
2. Aminaee, M.M., Ershad, D., 1999. Occurrence of Verticillium wilt on pistachio trees in Kerman province. Iranian Journal of Plant Pathology, 35(1-4): 59.
3. Anonim, 2008. Subtropik ve Sert Kabuklu Meyve Hastalıkları ve Zararlıları. Zirai Mücadele Teknik Talimatları, Cilt 5, Ankara.
4. Anonim, 2023. Bitkisel Üretim İstatistikleri. Türkiye İstatistik Kurumu, (https://data.tuik.gov.tr), (Erişim Tarihi: 10.05.2023).
5. Arie, T., 2019. Fusarium diseases of cultivated plants, control, diagnosis, and molecular and genetic studies. Journal of Pesticide Science, 44(4): 275-281.
6. Ashkan, M., Abusaidi, D., 1995. Pre and post-emergence damping-off of pistachio caused by Rhizoctonia solani. Proceedings of the 12th Iranian Plant Protection Congress, 2-7 September, Karaj, Iran, pp. 219.
7. Ashworth, L.Jr., Zimmerman, G., 1976. Verticillium wilt of the pistachio nut tree: Occurrence in California and control by soil fumigation. Phytopathology, 66(12): 1449-1451.
8. Aydın, M.H., 2019a. Siirt ilinde antepfıstığı (Pistacia vera L.) bahçelerinde hastalıkların tespiti. I. Uluslararası Harran Multidisipliner Çalışmalar Kongresi, 8-10 Mart, s. 85-86, Şanlıurfa, Türkiye.

9. Aydın, M.H., 2019b. Siirt ilinde fıstık (Pistacia vera L.) bahçelerinde izole edilen bazı fungusların patojenisitelerinin belirlenmesi. ISPEC Uluslararası Tarım ve Kırsal Kalkınma Kongresi, 10-12 Haziran, s. 243-248, Siirt, Türkiye.
10. Aydın, M.H., Turhan, G., 2009. Rhizoctonia solani’nin fungal antagonistlerinin belirlenmesi üzerinde araştırmalar. Anadolu Ege Tarımsal Araştırma Enstitüsü Dergisi, 19(2): 49-72.
11. Aydın, M.H., Ünal, F., 2021. Anastomosis groups and pathogenicity of Rhizoctonia solani Kühn ısolates obtained from pistachio (Pistacia vera L.) saplings in Siirt province, Turkey. Turkish Journal of Agricultural Research, 8(1): 18-26.
12. Baker, K.F., Cook, R.J., 1974. Biological Control of Plant Pathogens. W.H. Freeman and Company, San Francisco. Banihashemi, Z., 1995. Identifıcation of Phytophthora species associated with pistachio Gummosis in southern Iran. Acta Horticulturae, 419(58): 349-352.
13. Blodgett, J.T., Swart, W.J., Louw, S., 1998. First report of Fusarium sambucinum, F. oxysporum, and F. subglutinans associated with stem decay of Amaranthus hybridus in South Africa. Plant Diseases, 82(9): 1062.
14. Booth, C., 1971. Fusarium a Laboratory Guide to The Identification of The Major Species. C.M.I. Kew Surrey, England.
15. Bora, T., Karaca, İ., 1970. Kültür Bitkilerinde Hastalığın ve Zararın Ölçülmesi. Ege Üniversitesi Yardımcı Ders Kitabı, Yayın No: 167, Ege Üniversitesi Matbaası, Bornova-İzmir.
16. Chen, S.F., Morgan, D., Hasey, J.K, Anderson, K., Michailides, T., 2014. Phylogeny, morphology, distribution, and pathogenicity of Botryosphaeriaceae and Diaporthaceae from English walnut in California. Plant Disease, 98(5): 636-652.
17. Chitzanidis, A., 1995. Pistachio diseases in greece. Acta Horticulturae, 419(57): 345-348.
18. Cook, R.J., Baker, K.F., 1983. The Nature and Practice of Biological Control of Plant Pathogens. American Physical Society (APS), SZ Paul, Minnesoto.
19. Crespo Palomo, M., Lawrence, D., Nouri, M., Doll, D., Trouillas, F., 2019. Characterization of Fusarium and Neocosmospora species associated with crown rot and stem canker of pistachio rootstocks in California. Plant Disease, 103(8): 1931-1939.
20. Derviş, S., Türkölmez, Ş., Çiftçi, O., Serçe, Ç.U., Dikilitas, M., 2019. First report of Neoscytalidium dimidiatum causing canker, shoot blight, and root rot of pistachio in Turkey. Plant Diseases, 103(6): 1411-1411.
21. Doyle, J.J., Doyle, J.L., 1987. A rapid DNA isolation procedure from small quantities of fresh leaf tissues. Phytochemical Bulletin, 19(1): 11-15.
22. Epstein, L., Beede, R., Kaur, S., Ferguson, L., 2004. Rootstock effects on pistachio trees grown in Verticillium dahliae infested soil. Phytopathology, 94(4): 388-395.
23. Eskalen, A., Walter, G., Khan, A., 2001. Rootstock susceptibility to Phaemoniella chlamydospora and Phaeoacremonium spp. Phytopathologia mediterranea, 40(3): 433-438.
24. Fani, S.R., Zamanizadeh, H., Mirabolfathy, M., 2006. Root and crown rot of pistachio trees in Sistan and Baluchestan Provinces. Acta Horticulturae, 726(63): 647-651.
25. Frąc, M., Hannula, S.E., Bełka, M., Jędryczka, M., 2018. Fungal biodiversity and their role in soil health. Front Microbiology, 9(1): 707.
26. Ghosh, T., Biswas, M.K., Guin, C., Roy, P., 2018. A review on characterization, therapeutic approaches and pathogenesis of Macrophomina phaseolina. Plant Cell Biotechnology and Molecular Biology, 19(3-4): 72-84.
27. Güldür, M.E., Dikilitas, M., Ak, B.E., 2009. Pistachio diseases in the southeastern Anatolian region. Acta Horticulturae, 912: 739-742.
28. Hanlin, R.H., 2001. Illustrated Genera of Ascomycetes. Vol: 1.2, APS Press, The American Phytopathological Society, St. Paul, Minnesota.
29. Holtz, B.A., Michailides, T.J., Ferguson, L., Hancock, J.D., Weinhold, A.R.., 1996. First report of Rhizoctonia solani (AG-4) on pistachio rootstock seedlings in California. Plant Diseases, 80(11): 1303.
30. Ilkhan, L., Farokhi-Nejad, R., Aminaee, M., Behzadei-Rahimzadeh, H., 2011. Rhizoctonia root and crown rot of pistachio and its biological control in Kerman. Iranian Journal of Plant Pathology, 47(1): 93-99.
31. Iqbal, U., Mukhtar, T., 2014. Morphological and pathogenic variability among Macrophomina phaseolina isolates associated with mungbean (Vigna radiata L.) Wilczek from Pakistan. Scientific World Journal, 15: 950175.
32. Karaca, M., İnce, A.G., Elmasulu, S.Y., Onus, A.N., Turgut, K., 2005. Coisolation of genomic and organelle DNAs from 15 genera and 31 species of plants. Analytical Biochemistry, 343(2): 353-355.
33. Kaur, S., Dhillon, G.S., Brar, S.K., Vallad, G.E., Chand, R., Chauhan, V.B., 2012. Biology, economic importance and current diagnostic trends. Critical Review Microbiology, 38(2): 136-151.
34. Kurt, S., Uysal, A., Soylu, E.M., Kara, M., Soylu, S., 2019. First record of Neoscytalidium novaehollandiae associated with pistachio dieback and decline in the Southeastern Anatolia Region of Turkey. Mycologia Iranica, 6(1): 55-57.
35. Liu, J.K., Hyde, K.D., Jones, E.B.G., Ariyawansa, H.A., Bhat, D.J., Boonmee, S., Maharachchikumbura, S.S.N., McKenzie, E.H.C., Phookamsak, R., Phukhamsakda, C., Shenoy, B.D., Abdel-Wahab, M.A., Bart, B., 2015. Fungal diversity notes 1-110: Taxonomic and phylogenetic contributions to fungal species. Fungal Diversity, 72(1): 1-197.
36. Mahdikhani, M., Matinfar, M., Aghaalikhani, A., 2018. First report of Phytophthora palmivora causing crown and root rot of pistachio trees in northern Iran. Journal of Plant Pathology, 100(3): 583.
37. Michailides, T.J., Morgan, D.P., 2004. Panicle and Shoot Blight of Pistachio: A Majör Threat to the California Pistachio Industry. APSnet Feature. (https://www.apsnet.org/edcenter/apsnetfeatures/Pages/Pistachio.aspx), (Erişim Tarihi: 19.10.2023).
38. Michailides, T.J., Morgan, D.P., Doster, M.A., Kaska, N., Kuden, A.B., Ferguson, L., Michailides, T., 1995. Diseases of pistachio in California and their significance. Acta-Horticulturae, 419(56): 337-344.
39. Morgan, D.P., Epstein, L., 1992. Verticillium Wilt resistance in pistachio rootstock cultivars: Assay and assesment of two interspecific hybrids. Plant Disease, 76(3): 310-313.
40. Naffaa, W., Rasheed, A., 2017. First report of Fusarium solani causing root rot of pistachio (Pistacia vera L.) seedlings in nurseries in Syria. The Arab Journal for Arid Environments, 10(1-2): 32-36.
41. Nouri, M.T., Lawrence, D.P., Holland, L.A., Doll, D.A., Kallsen, C.E., Culumber, C.M., Trouillas, F.P., 2019. Identification and pathogenicity of fungal species associated with canker diseases of pistachio in California. Plant Diseases, 103(9): 2397-2411.
42. Nouri, M.T., Lawrence, D.P., Kallsen, C.E., Trouillas, F.P., 2020. Macrophomina crown and root rot of pistachio in California. Plants, 9(2): 134-152.
43. O’Hanlon, R., McCracken, A.R.., Cooke, L.R., 2016. Diversity and ecology of Phytophthora species on the island of Ireland. Biology and Environment: Proceedings of the Royal Irish Academy, 116B(1): 27-51.
44. Ören, E., Koca, G., Gencer, R., Bayraktar, H., 2020. First report of Neoscytalidium novaehollandiae associated with stem canker and branch dieback of almond trees. Australasian Plant Disease Notes, 15(1): 17.
45. Phillips, A.J.L., Alves, A., Abdollahzadeh, J., Slippers, B., Wingfield, M.J., Groenewald, J.Z. Crous, P.W., 2013. The Botryosphaeriaceae: Genera and species known from culture. Study in Mycology, 76(1): 51-167.
46. Samuels, G.J., 2006. Trichoderma: Systematics, the sexual state, and ecology. Phytopathology, 96(2): 195-206.
47. Saremi, H., Amiri, M.E., Mirabolfathi, M., 2010. Application of soil solarization for controlling soilborne fungal pathogens in newly established pistachio and olive orchards. International Journal Fruit Science, 10(2): 143-156.
48. Sarpkaya, K., Erkılıç, A., 2020. Studies on biology, epidemiology and control of Pseudocercospora pistacina in pistachio. Acta Horticulturae, 1269(5): 33-40.
49. Seifert, K., 1996. Fuskey: Fusarium Interactive Key. Agriculture and AgriFood, Canada.
50. Sheibani, A., 1995. Pistachio production in Iran. Acta Horticulturae, 419(27): 165-174.
51. Sneh, B., Burpee, L., Ogoshi, A., 1991. Identification of Rhizoctonia Species. The American Phytopathological Society, St. Paul, MN, USA.
52. Tatlı, F., 1996. Şanlıurfa antepfistığı alanlarında görülen fungal hastalık etmenlerinin saptanması. Yüksek Lisans Tezi, Harran Üniversitesi, Fen Bilimleri Enstitüsü, Şanlıurfa.
53. Teviotdale, B.L., Michailides, T.J., Pscheidt, J.W., 2002. Compendium of Nut Crop Diseases in Temperate Zones. The American Phytopathological Society, St. Paul, MN.
54. Triki, M., Rhouma, A., Chaabouni, A.C., Ioos, R., 2011. Emergence of Fusarium solani causing root rot of pistachio trees in Tunisia. International Society for Horticultural Science, 91(22): 717-721.
55. Trouillas, F.P., Nouri, M.T., Bourret, T.B., 2022. Identification and characterization of Phytophthora species associated with crown and root rot of pistachio trees in California. Plant Diseases, 106(1): 197-206.
56. Türkölmez, Ş., Çiftçi, O., Derviş, S., Ulubaş Serçe, Ç., 2015. First report of Phytophthora palmivora causing crown and root rot of pistachio trees in Turkey. Plant Disease, 99(12): 1866.
57. Zhang, X.Y., Yu, X.X., Yu, Z., Xue, Y.F., Qi, L.P., 2014. A simple method based on laboratory inoculum and field inoculum for evaluating potato resistance to black scurf caused by Rhizoctonia solani. Breeding Science, 64(2): 156-163.