Biostimulant Effects of Cladophora glomerata and Limnospira platensis on Growth and Rhizosphere Activity of Mentha spicata

Yıl 2026, Cilt: 13 Sayı: 1, 1 - 11, 14.03.2026

Göksal Sezen , Çiğdem Küçük , Reyyan Okutan

https://doi.org/10.19159/tutad.1742063

https://izlik.org/JA57GL72KP

Öz

This study aims to assess the biological properties of rhizosphere soil and the growth performance of Mentha spicata through the application of macroalgae and microalgae, promoting sustainable agriculture and environmental conservation. Seedlings were treated various concentrations (0%, 0.2%, 0.4%, and 0.6%) of C. glomerata as a macroalgae source and L. platensis as a microalgae source. Applications were carried out at 15-day intervals, starting one month after sowing. While the treatments varied in their effects on these growth factors, they significantly enhanced plant metrics compared to the control group. The highest chlorophyll content in leaves was achieved with the combined application of Cladophora glomerata (0.6%) and Limnospira platensis (0.6%). The findings demonstrated that microalgae and macroalgae treatments not only improved plant growth but also significantly increased β-glucosidase and dehydrogenase enzyme activities in the rhizosphere compared to the control (p<0.05). Additionally, soil chlorophyll levels were markedly elevated by algal treatments (p<0.05). The results showed that the combined application of C. glomerata and L. platensis is a promising biostimulant approach for improving mint growth and soil biological activity.

Anahtar Kelimeler

Microalgal-based biofertilizers , macroalgae , microalgae , microbial activity , organic agriculture

Kaynakça

• Anbi, A.A., Mirshekari, B., Eivazi, A., Yarnia, M., Behrouzyar, E.K., 2020. PGPRs affected photosynthetic capacity and nutrient uptake in different Salvia species. Journal of Plant Nutrition, 43(1): 108-121.
• Barone, V., Puglisi, I., Fragalà, F., Lo Piero, A.R., Giuffrida, F., Baglieri, A., 2019. Novel bioprocess for the cultivation of microalgae in hydroponic growing system of tomato plants. Journal of Applied Phycology, 31: 465-470.
• Bharti A., Prasanna, R., Kumar, G., Nain, L., Rana, A., Ramakrishnan, B., Shivay, Y.S., 2020. Cyanobacterium-primed chrysanthemum nursery improves performance of the plant and soil quality. Biology and Fertility of Soils, 57: 89-105.
• Bharti A., Prasanna, R., Kumar, G., Nain, L., Rana, A., Ramakrishnan, B., Shivay, Y.S., 2021. Cyanobacterial amendment boosts plant growth and flower quality in Chrysanthemum through improved nutrient availability. Applied Soil Ecology, 162: 103899.
• Cavallo, A., Giangrande, A., Accogli, R., Marchiori, S., 2006. A test on the use of Cladophora prolifera (Roth.) Kutz. (Chlorophyta, Cladophorales) as effective fertilizer for agricultural use. Thalassia Salentina, 29: 101-106.
• Ceylan, B., Sezen, G., 2024. Determination of biological activity of some macro/micro algae. Kastamonu University Journal of Engineering and Sciences, 10(1): 1-6.
• Chabili, A., Hakkoum, Z., Minaoui, F., Douma, M., Meddich, A., Loudiki, M., 2025. Germination screen of eco-extracts from soil cyanobacteria and microalgae for their biostimulant effects on wheat seeds emergence and vigor. Algal Research, 89: 104087.
• Chen, Y., Li, J., Huang, Z., Su, G., Li, X., Sun, Z., Qin, Y., 2020. Impact of short-term application of seaweed fertilizer on bacterial diversity and community structure, soil nitrogen contents, and plant growth in maize rhizosphere soil. Folia Microbiologica, 65(3): 591-603.
• Chookalaii, H., Riahi, H., Shariatmadari, Z., Mazarei, Z., Seyed Hashtroudi, M., 2020. Enhancement of total flavonoid and phenolic contents in Plantago major L. with plant growth promoting cyanobacteria. Journal of Agricultural Science and Technology, 22(2): 505-518.
• Daunoras, J., Kacergius, A., Gudiukaite, R., 2024. Role of soil microbiota enzymes in soil health and activity changes depending on climate change and the type of soil ecosystem. Biology (Basel), 13(2): 85.
• De Almeida, R.F., Naves, E.R., Da Mota, R.P., 2015. Soil quality: Enzymatic activity of soil β-glucosidase. Global Journal of Agricultural Research and Reviews, 3(2): 146-450.
• De Silva, A.G.S.D., Hashim, Z.K., Solomon, W., Zhao, J.B., Kovács, G., Kulmány, I.M., Molnár, Z., 2024. Unveiling the role of edaphic microalgae in soil carbon sequestration: potential for agricultural ınoculants in climate change mitigation. Agriculture, 14(11): 2065.
• Dziergowska, K., Wełna, M., Szymczycha-Madeja, A., Chęcmanowski, J., Michalak, I., 2021. Valorization of Cladophora glomerata biomass and obtained bioproducts into biostimulants of plant growth and as sorbents (biosorbents) of metal ions. Molecules, 26(22): 6917.
• Gamage, A., Gangahagedara, R., Gamage, J., Jayasinghe, N., Kodikara, N., Suraweera, P., Merah, O., 2023. Role of organic farming for achieving sustainability in agriculture. Farming System, 1(1): 100005.
• Gayathri, M., Shunmugam, S., Thajuddin, N., Muralitharan, G., 2017. Phytohormones and free volatile fatty acids from cyanobacterial biomass wet extract (BWE) elicit plant growth promotion. Algal Research, 26: 56-64.
• Geries, L.S.M., Elsadany, A.Y., 2021. Maximizing growth and productivity of onion (Allium cepa L.) by Spirulina platensis extract and nitrogen-fixing endophyte Pseudomonas stutzeri. Archives of Microbiology, 203(1): 169-181.
• Gezinç, H., Hasdemir, M., 2021. Medicinal and Aromatic Plants Sector Policy Document 2020-2024. General Directorate of Agricultural Research and Policies, Ankara, (https://www.tarimorman.gov.tr), (Accessed: 08.17. 2025). (In Turkish).
• Girard, A., Schweiger, A.K., Carteron, A., Kalacska, M., Laliberté, E., 2020. Foliar spectra and traits of bog plants across nitrogen deposition gradients. Remote Sensing, 12(15): 2448.
• Górka, B., Korzeniowska, K., Lipok, J., Wieczorek, P.P., 2018. The biomass of algae and algal extracts in agricultural production. In: K. Chojnacka, P. Wieczorek, G. Schroeder and I. Michalak (Eds.), Algae Biomass: Characteristics and Applications: Towards Algae-Based Products, Cham: Springer International Publishing, pp. 103-114.
• Jiajun, H., Hongcheng, G., Yiyun, X., Mintian, G., Shiping, Z., Tsang, Y.F., 2019. Using a mixture of microalgae, biochar, and organic manure to increase the capacity of soil to act as carbon sink. Journal of Soils Sediments, 19(11): 3718-3727.
• Khan, M.I., Shin, J.H., Kim, J.D., 2018. The promising future of microalgae: Current status, challenges, and optimization of a sustainable and renewable industry for biofuels, feed, and other products. Microbial Cell Factories, 17: 1-21.
• Küçük, Ç., Şinşek, N., 2020. The effects of different agricultural wastes on some microbiologial properties of soil. International on Mathematic, Engineering and Natural Sciences, 15: 451-460.
• Küçük, Ç., Uslu, P., Sezen, G., 2024. Cladophora sp. and Arbuscular Mycorrhizal Fungus (AMF) spore inoculation on maize (Zea mays L.) developmental parameters and some rhizosphere soil enzymes. Süleyman Demirel University Journal of Natural and Applied Sciences, 28(2): 189-196.
• Lopez-Rodriguez, A., Mayorga, J., Flaig, D., Fuentes, G., Hernandez, V., Gomez, P.I., 2023. Genetic characterization and assessment of the biotechnological potential of strains belonging to the genus Arthrospira/Limnospira (Cyanophyceae) deposited in different culture collections. Algal Research, 73: 103164.
• Mahadik, B.B., Kabnoorkar, P.S., 2020. Effect of different algal powder on growth and productivity of Moongbean (Phaseolus radiata). Flora and Fauna, 26: 22-28.
• Maizatul, A.Y., Radin Mohamed, R.M.S., Al-Gheethi, A.A., Hashim, M.K.A., 2017. An overview of the utilisation of microalgae biomass derived from nutrient recycling of wet market wastewater and slaughterhouse wastewater. International Aquatic Research, 9(3):177-193.
• Marks, E.A., Montero, O., Rad, C., 2019. The biostimulating effects of viable microalgal cells applied to a calcareous soil: Increases in bacterial biomass, phosphorus scavenging, and precipitation of carbonates. Science of the Total Environment, 692: 784-790.
• Michalak, I., Lewandowska, S., Detyna, J., Olsztyńska-Janus, S., Bujak, H., Pacholska, P., 2018. The effect of macroalgal extracts and near infrared radiation on germination of soybean seedlings: Preliminary research results. Open Chemistry, 16: 1066-1076.
• Michalak, I., Miller, U., Tuhy, Ł., Sówka, I., Chojnacka, K., 2017. Characterisation of biological properties of co-composted Baltic seaweeds in germination tests. Engineering in Life Sciences, 17: 153-164.
• Nayak, S., Prasanna, R., Pabby, A., Dominic, T.K., Singh, P.K., 2004. Effect of urea, blue green algae and Azolla on nitrogen fixation and chlorophyll accumulation in soil under rice. Biology and Fertility of Soils, 40: 67-72.
• Osorio-Reyes, J.G., Valenzuela-Amaro, H.M., Pizana-Aranda, J.J.P., Ramirez-Gamboa, D., Melendez-Sanchez, E.R., Lopez-Arellanes, M.E., Castaneda-Antonio, M.D., Coronado-Apodaca, K.G., Gomes Araujo, R., Sosa-Hernandez, J.E., Melchor-Martinez, E.M., Iqbal, H.M.N., Parra-Saldivar, R., Martinez-Ruiz, M., 2023. Microalgae-based biotechnology as alternative biofertilizers for soil enhancement and carbon footprint reduction: advantages and implications. Marine Drugs, 21(2): 93.
• Prasanna, R., Babu, S., Bidyarani, N., Kumar, A., Triveni, S., Monga, D., Mukherjee, A.K., Kranthi, S., Gokte-Narkhedhar, N., Adak, A., Yadav, K., Nain, L., Saxena, A.K., 2015. Prospecting cyanobacteria fortified composts as plant growth promoting and biocontrol agents in cotton. Experimental Agriculture, 51: 42-65.
• Prasanna, R., Kanchan, A., Ramakrishnan, B., Ranjan, K., Venkatachalam, S., Hossain, F., Shivay, Y.S., Krishnan, P., Nain, L., 2016. Cyanobacteria-based bioinoculants influence growth and yields by modulating the microbial communities favourably in the rhizospheres of maize hybrids. European Journal of Soil Biology, 75: 15-23.
• Ranjan, K., Priya, H., Ramakrishnan, B., Prasanna, R., Venkatachalam, S., Thapa, S., Shivay, Y.S., 2016. Cyanobacterial inoculation modifies the rhizosphere microbiome of rice planted to a tropical alluvial soil. Applied Soil Ecology, 108: 195-203.
• Riahi, H., Shariatmadari, Z., Heidari, F., Nohooji, M.G., Zarezadeh, S., 2023. Cyanobacterial elicitors as efficient plant growth promoters affect the biomass and metabolic profiles of four species of Mentha L.: A comparative study. South African Journal of Botany, 162: 568-576.
• Riba, M., Kiss-Szikszai, A., Gonda, S., Parizsa, P., Deák, B., Török, P., Valkó, O., Felföldi, T., Vasas, G., 2020. Chemotyping of terrestrial Nostoc-like isolates from alkali grassland areas by non-targeted peptide analysis. Algal Research, 46: 101798.
• Santini, G., Biondi, N., Rodolfi, L., Tredici, M.R., 2021. Cyanobacteria: An emerging strategy to improve yields and sustainability in agriculture. Plants, 10(4): 643.
• Sezen, G., Küçük, Ç., 2024. Effects of biochar and Cladophora glomerata treatments on wheat (Triticum aestivum L.) growth and rhizosphere enzyme activities. Commagene Journal of Biology, 8(2): 80-86.
• Shah, A., Smith, D.L., 2020. Flavonoids in agriculture: Chemistry and roles in, biotic and abiotic stress responses, and microbial associations. Agronomy, 10(8): 1209.
• Shakeela, S., Padder, S.A., Bhat, Z.A., 2017. Isolation and characterization of plant growth promoting rhizobacteria associated with medicinal plant Picrorhiza kurroa. Journal of Pharmacognosy and Phytochemistry, 6(3): 157-168.
• Shukla, P.S., Mantin, E.G., Adil, M., Bajpai, S., Critchley, A.T., Prithiviraj, B., 2019. Ascophyllum nodosum-based biostimulants: Sustainable applications in agriculture for the stimulation of plant growth, stress tolerance, and disease management. Frontiers in Plant Science, 10: 462648.
• Supraja, K.V., Behera, B., Balasubramanian, P., 2020. Efficacy of microalgal extracts as biostimulants through seed treatment and foliar spray for tomato cultivation. Industrial Crops and Products, 151: 112453.
• Tabatabai, M.A., 1982. Soil enzymes. In: A.L. Page (Ed.), Methods of Soil Analysis: Part 2 Chemical and Microbiological Properties, Agronomy Monographs, 9: 903-947.
• Win, T.T., Barone, G.D., Secundo, F., Fu, P., 2018. Algal biofertilizers and plant growth stimulants for sustainable agriculture. Industrial Biotechnology, 14(4): 203-211.
• Yeşil, M., Kara, K., 2014. Effect of nitrogen and phosphorus dosages on agricultural properties of Mentha spicata L. and Mentha villoso-nervata Opiz. genotypes. Academic Journal of Agriculture, 3(1): 23-32. (In Turkish).
• Zarezadeh, S., Riahi, H., Shariatmadari, Z., Sonboli, A., 2020. Effects of cyanobacterial suspensions as bio-fertilizers on growth factors and the essential oil composition of chamomile, Matricaria chamomilla L. Journal of Applied Phycology, 32: 1231-1241.