Determination of Optimum Duration and Concentration of Stevia (Stevia rebaudiana Bert.) Seed Priming with Boric Acid (H3BO3)

Yıl 2017, , 24 - 30, 28.02.2017

Mehdi Aghighi Shahverdı Heshmat Omıdı Seyed Jalal Tabatabaeı

https://doi.org/10.19159/tutad.300701

Cited By: 7

Öz

In order to determine optimal duration and concentration of Stevia seed priming with boric acid for improving
germination, an experimental factorial completely randomized design with three replications was conducted in the
laboratory of Seed Science and Technology, Shahed University of Tehran in 2015. The first factor was different
concentrations of boric acid (0, 0.5, 1.0, 1.5 and 2.0 percent) and the second factor was priming duration (0, 8, 16, 24 and 32
hours). Effects of concentration and priming duration with boric acid were significant on germination percentage, mean
germination time, germination rate, germination energy, germination uniformity, mean daily germination, germination daily
speed, germination value, seedling length and seed vigor index. The highest values for germination percentage, germination
rate, germination energy, means of daily germination, germination value and seedling vigor index were observed after 24
hours of boric acid priming. The highest germination percentage, germination rate, germination potential, germination value
and seed vigor index were obtained in 2.0% boric acid priming. 24 hours priming at 1.0% concentration of boric acid had
the highest germination uniformity. The seedling length was highest in 24 hours priming at 1.5% concentration of boric acid.
In general, Stevia seed priming with 1.5 to 2.0% of boric acid for 24 hours had positive effects on germination indexes and
seedling growth.

Anahtar Kelimeler

Boric acid, germination, micronutrients, nutri-priming, seed vigor

Determination of Optimum Duration and Concentration of Stevia (Stevia rebaudiana Bert.) Seed Priming with Boric Acid (H3BO3)

Öz

In order to determine optimal duration and concentration of Stevia seed priming with boric acid for improving germination, an experimental factorial completely randomized design with three replications was conducted in the laboratory of Seed Science and Technology, Shahed University of Tehran in 2015. The first factor was different concentrations of boric acid (0, 0.5, 1.0, 1.5 and 2.0 percent) and the second factor was priming duration (0, 8, 16, 24 and 32 hours). Effects of concentration and priming duration with boric acid were significant on germination percentage, mean germination time, germination rate, germination energy, germination uniformity, mean daily germination, germination daily speed, germination value, seedling length and seed vigor index. The highest values for germination percentage, germination rate, germination energy, means of daily germination, germination value and seedling vigor index were observed after 24 hours of boric acid priming. The highest germination percentage, germination rate, germination potential, germination value and seed vigor index were obtained in 2.0% boric acid priming. 24 hours priming at 1.0% concentration of boric acid had the highest germination uniformity. The seedling length was highest in 24 hours priming at 1.5% concentration of boric acid. In general, Stevia seed priming with 1.5 to 2.0% of boric acid for 24 hours had positive effects on germination indexes and seedling growth.

Anahtar Kelimeler

Boric acid, germination, micronutrients, nutri-priming, seed vigor

Kaynakça

• Abdollahi, M., Eshghi, S., Tafazzoli, E., Moosavi, N., 2012. Effects of paclobutrazol, boric acid and zinc sulfate on vegetative and reproductive growth of strawberry cv. Selva. Journal Agriculture Science Technology, 14(2): 357-363.
• Aghighi Shahverdi, M., Omidi, H., 2015. Effect of hormone priming and hydropriming on germination of Stevia (Stevia rebaudiana Bertoni) under salt stress. Iranian of Seed Sciences and Research, (In Press).
• Bayat, M., Rahmani, A., Amirnia, R., Ramazani, M., 2015. The effect of priming and duration of priming on germination indices and seedling characteristics of Althaea officinalis L. plant. Iranian Journal of Seed Science and Technology, 4(1): 73-82.
• Biradar, K.S., Salimath, P.M., Ravikumar, R.L., 2007. Genetic variability for seedling vigor, yield and yield components in local germplasm collections of Greengram [Vigna radiata (L.) Wilczek]. Karnataka Journal Agriculture Science, 20(3): 608-609.
• Deb, P., Das, A., Ghosh, S.K., Suresh, C.P., 2010. Improvement of seed germination and seedling growth of papaya (Carica papaya L.) through different pre-sowing seed treatments. Acta Horticulture, 851: 313-316.
• Ellis, R.H., Roberts, E.H., 1981. The quantification of aging and survival in orthodox seeds. Seed Science and Technology, 9: 377-409.
• Farooq, M., Basra, S.M.A., Warraich, E.A., Khaliq, A., 2006. Optimization of hydropriming techniques for rice seed invigoration. Seed Science and Technology, 34(2): 529-534.
• Farooq, M., Wahid, A., Siddique, K.H.M., 2012. Micronutrient application through seed treatments- a review. Journal of Soil Science and Plant Nutrition, 12(1): 125-142.
• Ghasemi Gholozani, K., Dalil, B., 2011. Germination and seed vigor tests. Publications Jahad Daneshgahi Mashhad.
• Goettemoeller, J., Ching, A., 1999. Seed germination in Stevia rebaudiana. In: Janick, J (Eds), Perspectives on new crops and new uses. ASHS Press, Alexandria, VA, pp: 510-511.
• Harris, D., Joshi, A., Khan, P.A., Gothkar, P., Sodhi, P.S., 1999. On-farm seed priming in semi-arid agriculture: Development and evaluation in maize, rice and chickpea in India using participatory methods. Experimental Agriculture, 35(1): 15-29.
• Hoogenboom, G., Peterson, C.M., Huck, M.G., 1987. Shoot growth rate of soybean as affected by drought stress. Agronomy Journal, 79(4): 598-607.
• Hossein, M.A., Shamim Kabri, A.M., Jahan, T.A., Hassan, M.N., 2008. Micropropagation of Stevia rebaudiana. International Journal of Sustainable Crop Production, 3: 1-90.
• Iqbal, S., Farooq, M., Nawaz, A., Rehman, A., 2012. Optimizing boron seed priming treatments for improving the germination and early seedling growth of wheat. Journal of Agriculture and Social Sciences, 8: 57-61.
• Johnson, S.E., Lauren, J.G., Welch, R.M., Duxbury, J.M., 2005. A comparison of the effects of micronutrient seed priming and soil fertilization on the mineral nutrition of chickpea (Cicer arietinum), lentil (Lens culinaris), rice (Oryza sativa) and wheat (Triticum aestivum) in Nepal. Experimental Agriculture, 41(4): 427-448.
• Kaur, S., Gupta, A.K., Kaur, N., 2005. Seed priming increase crop yield possibly by modulating enzymes of sucrose metabolism in chickpea. Journal of Agronomy and Crop Science, 191(2): 81-87.
• Liopa-Tsakalidi, A., Kaspiris, G., Salahas, G., Barouchas, P., 2012. Effect of salicylic acid (SA) and gibberellic acid (GA1) pre-soaking on seed germination of Stevia (Stevia rebaudiana) under salt stress. Journal of Medicinal Plants Research, 6(3): 416-423.
• Maiti, R.K., Purohit, S.S., 2008. Stevia: A miracle plant for human health Agrobios (India). Jodhpur India.
• McDonald, M.B., 2000. Seed priming. In M. Black, & J. D. Bewley (Ed.). Seed Technology and Biological Basis, Black (Chapter 9, pp: 287-325). Sheffield Academic Press, England.
• Memon, N.N., Gandahi, M.B., Pahoja, V.M., Sharif, N., 2013. Response of seed priming with Boron on germination and seedling sprouts of Broccoli. International Journal of Agriculture Science and Research, 3(2): 183-194.
• Mengel, K., Kirkby, E.A., Kosegarten, H., Appel, T., 2001. Principles of plant nutrition. Kluwer Academic Publishers, Dordrecht, the Netherlands.
• Mirshekari, B., 2012. Seed priming with iron and boron enhances germination and yield of dill (Anethum graveolens). Turkish Journal of Agriculture and Forestry, 36(1): 27-33.
• Oddone, B., 1997. How to Grow Stevia. Technical Manual. Guarani Botanicals, Pawtucket, CT.
• Pagter, M., Bragato, C., Malagoli, M., Brix, H., 2009. Osmotic and ionic effects of NaCl and Na2SO4 salinity on Phragmites australis. Aquatic Botany, 90(1): 43-51.
• Panwar, P., Bhardwaj, S.D., 2005. Handbook of practical forestry, Agrobios (INDIA).
• Raina, R., Bhandari, S.K., Chand, R., Sharma, Y., 2013. Strategies to improve poor seed germination in Stevia rebaudiana, a low calorie sweetener. Journal of Medicinal Plants Research, 7(24): 1793-1799.
• Raji, A.A., Mohammad, B.O., Zarina, B.Z., 2015. Acclimatized apparatus enhanced seed germination in Stevia rebaudiana Bertoni. International Journal of Biology, 7(2): 28-34.
• Rehman, A., Farooq, M., Cheema, Z.A., Wahid, A., 2012. Role of boron in leaf elongation and tillering dynamics in fine grain aromatic rice. Journal of Plant Nutrition, 38(3): 338-354.
• Rerkasem, B., Jamjod, S., 2004. Boron deficiency in wheat: A review. Field Crops Research, 89(2-3): 173-186.
• Robert, L.M., Timothy, K.H., Rusan, M.J., 2015. Challenges of increasing water and nutrient efficincy in irrigated agriculture, Chaper 8 of “Managing water and fertilizer for sustainable agricultural intensification”, International Fertilizer Industry Association, pp: 168-186.
• Rowse, H.R., 1995. Drum priming: A non-osmotic method of priming seeds. Seed Science and Technology, 24: 281-294.
• Shorrocks, V.M., 1997. The occurrence and correction of boron deficiency. Plant and Soil, 193(1): 121-148.
• Singh, S.D., Rao, G.P., 2005. Stevia: the herbal sugar of 21st century. Sugar Technology, 7(1): 17-24.
• Srivastava, S.P., Bhandari, T.M.S., Yadav, C.R., Joshi, M., Erskine, W., 2000. Boron deficiency in lentil: Yield loss and geographic distribution in a germplasm collection. Plant and Soil, 219(1): 147-151.
• Stephanie, E.B., Svoboda, V.P., Paul, A.T., Marc, W.V.I., 2005. Controlled drought affects morphology and anatomy of Salvia splendens. Journal of American Society for Horticultural Science, 130(5): 775-781.
• Tabrizian, F., Osareh, A.M., 2007. Improved seed emergence and yield related traits of marigold (Calendula officinalis L.) by on-farm seed micronutrient treatment trials. Iranian Journal of Crop Science, 9: 124-141.
• Tanaka, M., Fujiwar, T., 2008. Physiological roles and transport mechanisms of boron: Perspectives from plants. Pflügers Archiv-European Journal of Physiology, 456(4): 671-677.
• Ucar, E., Ozyigit, Y., Turgut, K., 2016. The effects of light and temperature on germination of Stevia (Stevia rebaudiana Bertoni) Seeds. Turkish Journal of Agricultural Research, 3(1): 37-40.