Screening of Oryza sativa L. for Hpt Gene and Evaluation of Hpt Positive Samples Using Houba Retransposon-Based IRAP Markers

Yıl 2017, , 59 - 64, 28.02.2017

Gözde Yüzbaşıoğlu Sevgi Maraklı Nermin Gözükırmızı

https://doi.org/10.19159/tutad.300702

Cited By: 5

Öz

Increasing world population needs to enhance agricultural production because of food starvation. Genetically
modified organism (GMO) is a way to solve this problem. During gene transfers, DNA is inserted into a plant’s genome in a
random way. This produces spontaneous genetic changes with movement of transposable elements, and even increases
variations. Houba was described as one of the active retrotransposons in rice. The aim of this study was to screen rice
samples collected from Turkey, and analyse Houba retrotransposon movements with IRAP technique in transgenic ones and
their controls. For this purpose, 71 different rice seeds obtained from different regions of Turkey were used for GMO
analysis. All samples were screened by real time PCR to test cauliflower mosaic virus (CaMV) 35S promoter (P-35S)
regions, T-NOS (nopaline synthase terminator) regions, figwort mosaic virus (FMV) regions, bar, pat and Cry1ab/ac, and
hpt (hygromycin resistance) genes. Hpt gene was identified in 6 samples as a result of real time PCR analysis. These 6
transgenic samples with their controls were used for IRAP-PCR analysis and 0-56% polymorphism ratios were observed in
analysed samples. This study is one of the first detailed experimental data of transgenic Oryza sativa L. samples in terms of
retrotransposon-based variation. 

Anahtar Kelimeler

Genetically modified organism, Houba, IRAP, Real-Time PCR, rice

Screening of Oryza sativa L. for Hpt Gene and Evaluation of Hpt Positive Samples Using Houba Retransposon-Based IRAP Markers

Öz

Increasing world population needs to enhance agricultural production because of food starvation. Genetically modified organism (GMO) is a way to solve this problem. During gene transfers, DNA is inserted into a plant’s genome in a random way. This produces spontaneous genetic changes with movement of transposable elements, and even increases variations. Houba was described as one of the active retrotransposons in rice. The aim of this study was to screen rice samples collected from Turkey, and analyse Houba retrotransposon movements with IRAP technique in transgenic ones and their controls. For this purpose, 71 different rice seeds obtained from different regions of Turkey were used for GMO analysis. All samples were screened by real time PCR to test cauliflower mosaic virus (CaMV) 35S promoter (P-35S) regions, T-NOS (nopaline synthase terminator) regions, figwort mosaic virus (FMV) regions, bar, pat and Cry1ab/ac, and hpt (hygromycin resistance) genes. Hpt gene was identified in 6 samples as a result of real time PCR analysis. These 6 transgenic samples with their controls were used for IRAP-PCR analysis and 0-56% polymorphism ratios were observed in analysed samples. This study is one of the first detailed experimental data of transgenic Oryza sativa L. samples in terms of retrotransposon-based variation. 

Anahtar Kelimeler

Genetically modified organism, Houba, IRAP, Real-Time PCR, rice

Kaynakça

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