Cholinesterase, α-Glucosidase, α-Amylase, and Tyrosinase Inhibitory Effects and Antioxidant Activity of Veronica officinalis Extracts

Öz

In this study, inhibition ability of Veronica officinalis extracts against Alzheimer’s disease-related enzymes acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE), diabetes mellitus related enzymes α-glucosidase and α-amylase and antioxidant of Veronica officinalis were investigated. To the best of our knowledge, there are no previous studies on the enzyme inhibition activities of the V. officinalis extracts. For this aim, V. officinalis extracted with methanol and water by maceration method and their antioxidant activities were evaluated by DPPH (2,2-Diphenyl-1-picrylhydrazyl), ABTS [2,2′-Azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid)] radical scavenging assays, total phenol, total flavonoid content, iron chelating and ferric reducing power assays. The enzyme inhibition activity was performed with 96-well plate method. According to the obtained results; the plant extracts have good antioxidant effects. In the same time, the water extract have stronger inhibition activity against AChE, BuChE and α-glucosidase, α-amylase, and tyrosinase than methanol extract. The experimental findings indicated that the water extract exerted the higher α-glucosidase, α-amylase and tyrosinase (82.07 ± 2.91, 63.61 ± 3.93 and 47.47 ± 0.53 at 2 mg mL^-1, respectively) inhibition than reference drugs. The obtained results demonstrate that this plant has a significant potential for improving pharmaceutical formulations. 

Anahtar Kelimeler

• Antioxidant
• alzheimer’s
• diabetes
• tyrosinase
• Veronica officinalis

Kaynakça

1. Albach, D.C., Meudt, H.M., 2010. Phylogeny of veronica in the southern and northern hemispheres based on plastid, nuclear ribosomal and nuclear low-copy DNA. Molecular Phylogenetics and Evolution, 54(2): 457-471.
2. Beara, I., Živković, J., Lesjak, M., Ristic, J., Savikin, K., Maksimovic, Z., Jankovic, T., 2015. Phenolic profile and anti-inflammatory activity of three Veronica species. Industrial Crops and Products, 63: 276-280.
3. Chai, T., Mohan, M., Ong, H., Wong, F., 2014. Antioxidant, iron-chelating and anti-glucosidase activities of Typha domingensis Pers (Typhaceae). Tropical Journal of Pharmaceutical Research, 13(1): 67-72.
4. Clarke, G., Ting, K.N., Wiart, C., Fry, J., 2013. High correlation of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, ferric reducing activity potential and total phenolics content indicates redundancy in use of all three assays to screen for antioxidant activity of extracts of plants from the Malaysian Rainforest. Antioxidants, 2(1): 1-10.
5. Ellman, G.L., Courtney, KD., Andres, V., Featherstone, R.M., 1961. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical Pharmacology, 7(2): 88-90.
6. Emerit, J., Edeas, M., Bricaire, F., 2004. Neurodegenerative diseases and oxidative stress. Biomedicine & Pharmacotherapy, 58(1): 39-46.
7. Ertaş, A., Gören, A.C., Boğa, M., Yeşil, Y., Kolak, U., 2014. Essential oil compositions and anticholinesterase activities of two edible plants Tragopogon latifolius var. angustifolius and Lycopsis orientalis. Natural Product Research, 28(17): 1405-1408.
8. Halliwell, B., 1987. Oxidants and human disease: Some new concepts. Federation of American Societies for Experimental Biology, 1(5): 358-364.

9. Harput, U.Ş., Genç, Y., Khan, N., Saracoglu, I., 2011. Radical scavenging effects of different Veronica species. Records of Natural Products, 5(2): 100-107.
10. Havsteen, B.H., 2002. The biochemistry and medical significance of the flavonoids. Pharmacology & Therapeutics, 96(2-3): 67-202.
11. Jeong, S.H., Ryu, Y.B., Curtis-Long,, M.J., Ryu, H.W., Baek, Y.S., Kang, J.E., Lee, W.S., Park, K.H., 2009. Tyrosinase inhibitory polyphenols from roots of Morus lhou. Journal of Agricultural and Food Chemistry, 57(4): 1195-1203.
12. Junqueira, V.B., Barros, S.B., Chan, S.S., Rodriques, L., Giavarotti, L., Abud, R.L., Deucher, G.P., 2004. Aging and oxidative stress. Molecular Aspects of Medicine, 25(1-2): 5-16.
13. Kiss, B., Popa, D.S., Crişan, G., Bojiţǎ, M., Loghin, F., 2009. The evaluation of antioxidant potential of Veronica officinalis and Rosmarinus officinalis extracts by monitoring malondialdehide and glutathione levels in rats. Farmacia, 57(4): 432-441.
14. Kumar, D., Gupta, N., Ghosh, R., Gaonkar, R.H., Pal, B.C., 2013. α-Glucosidase and α-amylase inhibitory constituent of Carex baccans: Bio-assay guided isolation and quantification by validated RP-HPLC-DAD. Journal of Functional Foods, 5(1): 211-218.
15. Kumar, D., Kumar, H., Vedasiromoni, J.R., Pal, B.C., 2012. Bio- assay guided isolation of a-glucosidase inhibitory constituents from Hibiscus mutabilis leaves. Phytochemical Analysis, 23(5): 421-425.
16. Miser-Salihoglu, E., Akaydin, G., Caliskan-Can, E., Yardim-Akaydin, S., 2013. Evalution of antioxidant activity of various herbal folk medicines. Journal of Nutrition Food Sciences, 3(5): 222.
17. Mocan, A., Vodnar, D.C., Vlase, L., Crișan, O., Gheldiu, A.M., Crișan, G., 2015. Phytochemical characterization of Veronica officinalis L., V. teucrium L. and V. orchidea crantz from Romania and their antioxidant and antimicrobial properties. International Journal of Molecular Science, 16(9): 21109-21127.
18. Molan, A.L., Mahdy, A.S., 2014. Iraqi medicinal plants: Total flavonoid contents, free-radical scavenging and bacterial beta-glucuronidase inhibition activities. Journal of Dental and Medical Sciences, 13(5): 72-77.
19. Raclariu, A.C., Mocan, A., Popa, M.O., Vlase, L., Ichim, M.C., Crisan, G., Brysting, A.K, Boer H., 2017. Veronica officinalis product authentication using DNA metabarcoding and HPLC-MS reveals widespread adulteration with Veronica chamaedrys. Frontiers in Pharmacology, 8: 378, doi: 10.3389/fphar.2017.00378.
20. Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice-Evans, C., 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology & Medicine, 26(9-10): 1231-1237.
21. Rice-Evans, C., 2004. Flavonoids and isoflavones: absorption, metabolism, and bioactivity. Free Radical Biology & Medicine, 36(7): 827-828.
22. Supritha, P., Radha, K.V., 2018. Estimation of phenolic compounds present in the plant extracts using high pressure liquid chromatography , antioxidant properties and its antibacterial activity. Indian Journal of Pharmaceutical Education and Research, 52(2): 321-326.
23. Taskova, R.M., Gotfredsen, C.H., Jensen, S.R., 2006. Chemotaxonomy of Veroniceae and its allies in the Plantaginaceae. Phytochemistry, 67(3): 286-301.
24. Taskova, R., Peev, D., Handjieva, N., 2002. Iridoid glucosides of the genus Veronica s.l. and their systematic significance. Plant Systematics and Evolution, 231(1): 1-17.
25. Thilagam, E., Parimaladevi, B., Kumarappan, C., Chandra Mandal, S., 2013. α-Glucosidase and α-Amylase inhibitory activity of Senna surattensis. JAMS Journal of Acupuncture and Meridian Studies, 6(1): 24-30.
26. Wojdylo, A., Oszmianski, J., Czemerys, R., 2007. Antioxidant activity and phenolic compounds in 32 selected herbs. Food Chemistry, 105(3): 940-949.
27. Yang, H., Dong, Y., Du, H., Shi, H., Peng, Y., Li, X., 2011. Antioxidant compounds from propolis collected in Anhui, China. Molecules, 16(4): 3444-3455.
28. Zhou, J., Tang, Q., Wu, T., Cheng, Z., 2017. Improved TLC bioautographic assay for qualitative and quantitative estimation of tyrosinase inhibitors in natural products. Phytochemical Analysis, 28(2): 115-124.