Sorgum [Sorghum bicolor (L.) Moench] Genomunda BES1 Transkripsiyon Faktör Ailesinin Genom Çaplı Analizi

Öz

BES1 transkripsiyon faktörü ailesi brassinosteroidlerin biyosentezinde önemli bir role sahiptir. Bitki büyüme ve gelişme

süreçlerini ve çevresel streslere yanıt mekanizmasını etkileyen bir steroid hormonudur. Bu çalışmanın amacı sorgum

[Sorghum bicolor (L.) Moench] bitkisinin farklı dokularında farklı azot kaynakları (kontrol gübresi, amonyak, nitrat, üre)

uygulanarak bu dokulardaki BES1 transkripsiyon faktörünün ifade profillerini belirlemek ve in siliko olarak BES1 gen

ailesinin üyelerini genom çapında tespit ederek karakterize etmektir. Sorgum genomunda amino asit sayıları 190 ile 716,

moleküler ağırlıkları 35.27 ile 80.54 kDa ve izoelektrik noktaları 5.0 ile 10.07 arasında değişen 9 Sobic-BES1 proteini

belirlenmiştir. Gen yapısı analizlerinde tahmini ekzonların sayısı 2 ile 10 arasında değişmiştir. S. bicolor, Arabidopsis

thaliana ve Oryza sativa türlerinin BES1 proteinleri kullanılarak filogenetik ilişki tespit edilmiştir. Evrimsel süreçte Sobic-

BES1-4 ve Sobic-BES1-9 genlerinin segmental duplike olduğu belirlenmiştir. İn siliko gen ifade analizlerine göre farklı azot

kaynaklarının ve su kontrolünün uygulandığı kök ve sürgün dokularında Sobic-BES1-4 ve -9 genlerinin ifade seviyelerinin

en yüksek olduğu, diğer taraftan kullanılan azot kaynağına ve dokuya göre Sobic-BES1-1, Sobic-BES1-2 ve Sobic-BES1-8

genlerinin ifade seviyelerinin farklılık gösterdiği belirlenmiştir. Bu çalışmanın sonuçları fonksiyonel gen araştırmaları için

bir temel sağlayacak olup, sorgum bitkisinde BES1 gen ailesinin anlaşılmasına katkı sunacaktır.

Anahtar Kelimeler

• Biyoinformatik analizler,fitohormon,brassinosteroid,gen ailesi,gen ifadesi

Genome-Wide Analysis of BES1 Transcription Factor Family in Sorghum [Sorghum bicolor (L.) Moench] Genome

Öz

The BES1 transcription factor family has an important role in the biosynthesis of brassinosteroids. It is a steroid

hormone that affects plant growth and development processes and the mechanism of response to environmental stresses. The

aim of this study was to determine the expression profiles of BES1 transcription factor in different tissues of sorghum

[Sorghum bicolor (L.) Moench] by applying different nitrogen sources (controlled fertilized, ammonia, nitrate and urea) and

to characterize BES1 gene family members through in silico methods. In sorghum genome, 9 Sobic-BES1 proteins with

amino acid numbers ranged from 190 and 716, molecular weights between 35.27 and 80.54 kDa and isoelectric points

between 5.0 and 10.07 were determined. In gene structure analysis, the number of estimated exons ranged from 2 to 10.

Phylogenetic relationship was determined using BES1 proteins of S. bicolor, Arabidopsis thaliana and Oryza sativa species.

Sobic-BES1-4 and Sobic-BES1-9 genes were found to be segmental duplicated in the evolutionary process. According to the

silico gene expression analysis, the expression levels of Sobic-BES1-4 and -9 genes were the highest in the root and shoot

tissues where different nitrogen sources and irrigation control were applied. On the other hand, the expression levels of

Sobic-BES1-1, Sobic-BES1-2 and Sobic-BES1-8 genes were differed according to the nitrogen source and tissue used. The

results of this study will provide a basis for functional gene research and contribute to the understanding of the BES1 gene

family in Sorghum bicolor.

Anahtar Kelimeler

• Bioinformatic analysis,phytohormone,brassinosteroids,gene family,gene expression

Kaynakça

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