Extreme Processing Conditions Applied in Selection of Different Microorganisms Utilized as Compost Enhancers

Yıl 2018, Cilt: 5 Sayı: 3, 209 - 221, 31.10.2018

Fadime Özdemir Koçak , Levent Değirmenci

https://doi.org/10.19159/tutad.407623

Öz

Present
study included isolation of microorganisms during the mesophilic stage of composting and their reuse in compost mixture
to enhance organic matter degradation. Effect of microorganism addition on
compost degradation was investigated in the presence of microorganisms obtained
by isolation from a miniscule composting system. Effects of water addition and
time of mixing were applied as extreme composting conditions. Isolates were
obtained from these systems and inoculated to determine the most resistant
microorganisms. 16S rRNA sequencing of selected strains indicated the presence
of Escherichia, Enterococcus and Streptomyces
genus. These microorganisms were added to a large-scale compost mixture to
accelerate degradation of organic matter. Results indicated 20 and 38%
decreases of organic matter in the sole
utilization of Streptomyces sp. K1P07
and ternary utilization at the end of 14 days. Escherichia and Enterococcus inhibited
degradation in the case of binary utilization. Isolation of microorganisms from
the original flora of the compost mixture had accelerated composting due to
interaction among themselves. Member of Actinomycetes, in particular, was shown
to be effective even in the case of sole utilization. Higher amounts of
biomass-oriented wastes could be removed since composting would be terminated
in shorter times with the use of these microorganisms. 

Anahtar Kelimeler

16S rRNA gene sequencing, Escherichia sp., Enterococcus sp., Streptomyces sp., composting

Kaynakça

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