Fıstık (Pistacia vera L.) Yaprağından Gümüş Nanopartikül (AgNP)’lerin Sentezi, Karakterizasyonu ve Antimikrobiyal Aktivitesinin İncelenmesi

Yıl 2019, Cilt: 6 Sayı: 2, 165 - 173, 30.06.2019

Abdullah Eren Mehmet Fırat Baran

https://doi.org/10.19159/tutad.493006

Cited By: 7

Öz

Bu çalışmanın amacı, fıstık (Pistacia vera L.) bitki ekstraktı kullanılarak, yeşil sentez yöntemi ile gümüş nanopartikül (AgNP)’lerini
sentezlemektir. Yeşil sentez; çevre dostu ve maliyet açısından ucuz olması, kimyasal ve fiziksel yöntemlerden daha çok tercih edilen nanopartiküllerin
kolay bir şekilde elde edilmesi ile bilinen biyolojik bir yöntemdir. Reaksiyon sonucunda elde edilen AgNP’ler, UV görünür spektrofotometre (UV-vis),
fourier-dönüştürülmüş kızılötesi spektroskopisi (FTIR), X-Işını kırınımı (XRD), termogravimetrik ve diferansiyel termal analizi (TGA-DTA), taramalı elektron
mikroskobu ve enerji yayılımlı X-ışını cihazı (SEM-EDX) kullanılarak karakterize edilmiştir. Gümüş nanopartiküllerin 460.67 nm’de maksimum absorbansa sahip
olduğu görülmüştür. AgNP’lerin indirgenmesinde rol olan fitokimyasalları analiz etmek için FTIR analizi yapılmıştır. Debye-Scherrer’s denkleminden yararlanarak
sentezlenen nanoparçacıkların kristal boyutunun 16.7 nm olduğu hesaplanmıştır. AgNP’lerin küresel görünümde olduğu transmisyon elektron mikroskobu ile tespit
edilmiştir. Sentezlenen AgNP’lerin antimikrobiyal etkisi gram pozitif ve gram negatif bakterileri ve fungus (maya) üzerinde minimum inhibitör konsantrasyonu
(MIC) metodu ile test edilmiştir. Sonuç olarak, sentezlenen gümüş nanopartiküllerin antimikrobiyal etki gösterdiği belirlenmiştir.

Anahtar Kelimeler

Antimikrobiyal aktivite, AgNP, yeşil sentez, Pistacia vera L.

Synthesis, Characterization and Investigation of Antimicrobial Activity of Silver Nanoparticles (AgNP) from Pistachio (Pistacia vera L.) Leaf

Öz

The aim of this study is to synthesize silver nanoparticles (AgNPs) by green synthesis methods using pistachio (Pistacia vera L.) plant extract. Green synthesis is a biological method in which nanoparticles are easily obtained; as well as being environmentally friendly and inexpensive in terms of cost, and being more preferred than chemical and physical methods. AgNPs obtained by the reaction were chacarterized with UV visible spectrophotometer (UV-vis), fouriertransformed infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermogravimetric and differential thermal analysis (TGA-DTA), scanning electron microscopy and energy dispersive X-ray device (SEM-EDX). Silver nanoparticles were found to have a maximum absorbance at 460.67 nm. FTIR analysis was performed to analyze the phytochemicals involved in the reduction of AgNPs. Using the Debye-Scherrer's equation, the crystal size of the synthesized nanoparticles was calculated as 16.7 nm. AgNPs spherical appearance were detected by transmission electron microscopy. The antimicrobial effect of synthesized AgNPs were tested on gram-positive and gram-negative bacteria, and by the minimum inhibitory concentration (MIC) method on fungi (yeast). As a result, it was determined that the synthesized silver nanoparticles had antimicrobial effect.

Anahtar Kelimeler

Antimicrobial activity, AgNP, green synthesis, Pistacia vera L.

Kaynakça

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