Isolation of First Local Coranavirus from Cattle with Winter Dysentery in Turkey

Öz

Kış dizanterisi sulu ishal ile karakterize sığırların çok bulaşıcı bir hastalığıdır. Hastalığın etiyolojisi hala tam olarak aydınlatılamamıştır. Sunulan çalışmanın amacı kış dizanterisi ile enfekte olan sürüde etiyolojik ajanların, rutin hematolojik ve biyokimyasal parametrelerin araştırılmasıdır. Çalışmada Bursa bölgesinde tipik kış dizanterisi bulguları gösteren 12 sığırdan kan ve dışkı örnekleri toplanmıştır. Örnekler, bovine coronavirus (BCoV), bovine viral diarrhea (BVD), Eimeria ookistleri ve Campylobacter spp. yönünden ELISA, RT-PCR, kültür ve flotasyon tekniği ile analiz edilmiştir. Dışkı örneklerinde, virus izolasyonu için HRT hücre kültürü yapılmıştır. Örneklerin%25’inden ELISA, PCR ve serum nötralizasyon yöntemi ile coronavirus identifiye edilmiştir. Dizanterili sığırların %33,3’ünden Campylobacter spp. izole edilmiştir. Hiçbir örnekte BVD ve Eimeria ookistlerine rastlanılmamıştır. Sunulan çalışmanın sonuçları göstermektedir ki Campylobacter spp ile birlikte BcoV sığırlarda kış dizanterisinin primer etkeni olabilir. Ancak dışkı örneklerinin % 58’i BCoV ve Campylobacter spp yönünden negatiftir ve Department of Internal Medicine, Faculty of Veterinary Medicine, Uludag University, Bursa. [e-mail] Etlik Veterinary Control and Research İnstitute Ankara. Department of Parasitology, Faculty of Veterinary Medicine, Uludag University, Bursa. Pendik Veterinary Control and Research İnstitute İstanbul. Nuclear Safety Department, Turkish Atomic Energy Authority. Ankara. Ministry of Food Agriculture and Livestock, Manyas, Balıkesir. hastalığın etiyolojisi hala tam olarak aydınlatılamadığı için BCoV ve Campylobacter spp dışında başka pathojenlerde hastalığın pathogenezinde rol olabilir.

Anahtar Kelimeler

• Campylobacter spp, sığır, coronavirus, Kış Dizanterisi.

Isolation of First Local Coranavirus from Cattle with Winter Dysentery in Turkey

Öz

Winter dysentery (WD) is a very contagious disease of cattle characterized by profuse diarrhea. The precise etiology of disease is still not fully elucidated. Aim of the presented study was investigation of the causative agents of disease in a herd affected by WD and to evaluate routine haematological and biochemical parameters of cows with WD. This study was carried out on blood and fecal samples collected from 12 cows showing typical signs of disease during an outbreak of WD in Bursa, Turkey. Samples were analysed for the presence of bovine coronavirus (BCoV), Bovine viral diarrhea (BVD), Eimeria oocysts and Campylobacter spp. using ELISA, RT-PCR, flotation technique and culture. Faecal samples were inoculated in HRT cell cultures for virus isolation. Isolated viruses were identified as coronaviruses from 25% of the samples by ELISA, PCR and serum neutralisation tests. Campylobacter spp. was isolated from 33.3% cows with dysentery. BVD and Eimeria oocysts were not detected in any of the samples. Results of the presented study indicates that BcoV along with Compylobacter spp. may be the primary agent of WD in cows. However negative results for BCoV and Campylobacter spp of 58 % of feacal samples indicates that etiology of the disease is still not fully elucidated and pathogens other than BCoV and Campylobacter could also be involved in pathogenesis of the disease

Anahtar Kelimeler

• Campylobacter, cattle, coronavirus, winter dysentery.

Kaynakça

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