Araştırma Makalesi
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Süt, Et, İdrar ve Yemde Altı Farklı Grup Antimikrobiyal İlacın Biochip Array-Based Immunoassay ile Aynı Anda Tespiti

Yıl 2012, Cilt: 31 Sayı: 2, 29 - 33, 01.06.2012

Öz

Antimikrobiyaller besin üretiminde faydalandığımız hayvanlarda yaygın olarak kullanılmaktadır. Bu nedenle, bu besinlerde bulunabilen antimikrobiyal kalıntıları insan sağlığı açısından önemlidir. Farklı antimikrobiyal kalıntılarının aynı numunede ve aynı anda birlikte tespiti önemlidir. Bu nedenle, bu çalışmanın amacı Biochip array-based immunoassay yöntemi ile altı farklı grup antibakteriyel ilacın süt, et, idrar ve yemde deneysel şartlarda tespit etmektir. Bu test, aynı anda altı farklı grup antimikrobiyalin süt, idrar, et, bal ve yem numunelerinde miktarlarının belirlenmesinde kullanılmaktadır. Bu metodla hedef ilaçlar olan norfloksasin, seftifor, florfenikol, streptomisin, taylosin ve tetrasiklin süt, et, idrar ve yemde tespit edildi. Sonuç olarak, bu test ile yukarıda belirtilen altı farklı gruptaki hedef antibakteriyel ilaç süt, et, idrar ve yemde etkili bir şekilde tespit edilebilmektedir. Bu metod, belirtilen numunelerde bu antibakteriyel ilaçların kalıntılarının rutin analizinde kullanılabilir

Kaynakça

  • Botsoglou, N.A., Fletouris, D.J., 2001. Drug Residues in Foods, Pharmacology, Food Safety, and Analysis. Marcel Dekker, New York.
  • Chen, C.L., Gu, X., 1995. Determination of tetra- cycline residues in bovine milk, serum, and urine by capillary electrophoresis. J AOAC Int., 78(6), 1369-77.
  • Council Directive 96/23/EC, 1996. On measures to monitor certain substances and residues there- of in live animals and animal products and re- pealing Directives 85/358/EEC and 86/469/EEC and Decisions 89/187/EEC and 91/664/EEC. Off J Eur Commun., L125, 10-33.
  • Council Regulation (EEC) No 2377/90, 1990. Laying down a Community procedure for the es- tablishment of maximum residue limits of veteri- nary medicinal products in oodstuffs of animal origin. Off J Eur Commun., L 224, 1-8.
  • Honkanen-Buzalski, T., Reybroeck, W., 1997. Antimicrobials. Monograph on Residues and Contaminants in Milk and Milk Products. IDF Special Issue 9701. International Dairy Federa- tion, Brussel: 26–33.
  • Huebra, M.J.G., Bordin, U.V.G., Rodriguez, A.R., 2005. Determination of macrolide antibiot- ics in porcine and bovine urine by high- performance liquid chromatography coupled to coulometric detection. Anal Bioanal Chem., 382: 433–439.
  • Huet, A.C, Fodey, T., Haughey, S.A., Weigel, S., Elliott, C., Delahaut, P., 2010. Advances in bio- sensor-based analysis for antimicrobial residues in foods. Trends Anal Chem., 29 (11), 1281- 1294.
  • Kaya, S.E., Filazi, A., 2010. Determination of Antibiotic Residues in Milk Samples. Kafkas Univ Vet Fak Derg., 16 (Suppl-A), S31-S35.
  • Mäyrä-Mäkinen , A., 1995. Technological signif- icance of residues for the dairy industry. In: Symposium on Residues of Antimicrobial Drugs and Other Inhibitors in Milk. IDF Special Issue No. 95 05. Kiel, Germany: 136–143.
  • Navrátilová, P., 2008. Screening methods used for the detection of veterinary drug residues in raw cow milk – a review. Czech J Food Sci., 26, 393–401.
  • Omeiza, G.K., Ajayi, I.E., Ode, O.J., 2012. Assesment of antimicrobial drug residues in beef in Abuja, the Federal Capital Territory. Vet Ital- iana, 48 (3), 283-289.
  • Oruç, H. H., Rumbeiha, W. K., Ensley, S., Wol- sen, C., Schrunk, D. E. Simultaneous Detection of Six Different Groups of Antimicrobial Drugs in Porcine Oral Fluids Using a Biochip Array- Based Immunoassay. Kafkas Univ Vet Fak Derg., (DOI: 10.9775/kvfd.2012.7905).
  • Oruç, H.H., Cengiz, M., Bağdaş, D., Uzunoğlu, İ., 2006. Sığır Etlerinde Streptomisin ve Sulfa- metazin (Sulfadimidin) Kalıntıları (Streptomycin and Sulfamethazin (Sulphadimidine) Residues in Meat). Uludag Univ J Fac Vet Med., 26 (1-2), 17-20.
  • Özcan, A., Karaca, M.Y., Bayraktar, D., 2011. Güney Marmara Bölgesinde Satışa Sunulan Hayvan Yemlerindeki Antibiyotik ve Antikoksid- iostat Düzeylerinin LC-MS/MS Yöntemi İle Be- lirlenmesi Üzerine Bir Araştırma. Gıda Yem Bil Tek Derg., 8(11),1-15.
  • Randox Manual, 2011. Antimicrobial Array II (AM II), EV 3524 Manual.

Simultaneous Detection of Six Different Groups of Antimicrobial Drugs in Milk, Meat, Urine, and Feed Matrices

Yıl 2012, Cilt: 31 Sayı: 2, 29 - 33, 01.06.2012

Öz

Antimicrobials are widely used for animal health in food-producing animals. Therefore, antimicrobial residues are of food safety concern. Simultaneous detection of different antimicrobial residues in the same matrice is important. Therefore, the objective of this study was to detect six different group antimicrobials in milk, meat, urine, and feed matrices under experimental conditions, and to evaluate its use for routine analysis. Biochip array-based immunoassay is currently used for simultaneous detection and quantitation of different groups of six antimicrobials in milk, urine, meat, honey, and feed. Results showed that of the six target drugs, norfloxacin, ceftiofur, florfenicol, streptomycin, tylosin and tetracycline could be detected in milk, meat, urine, and in feed matrices. In conclusion, this assay can effectively detect all target antibacterials from different groups in milk, meat, urine, and feed matrices, and this assay can be used for routine detection of these antibacterial residues in the stated matrices.

Kaynakça

  • Botsoglou, N.A., Fletouris, D.J., 2001. Drug Residues in Foods, Pharmacology, Food Safety, and Analysis. Marcel Dekker, New York.
  • Chen, C.L., Gu, X., 1995. Determination of tetra- cycline residues in bovine milk, serum, and urine by capillary electrophoresis. J AOAC Int., 78(6), 1369-77.
  • Council Directive 96/23/EC, 1996. On measures to monitor certain substances and residues there- of in live animals and animal products and re- pealing Directives 85/358/EEC and 86/469/EEC and Decisions 89/187/EEC and 91/664/EEC. Off J Eur Commun., L125, 10-33.
  • Council Regulation (EEC) No 2377/90, 1990. Laying down a Community procedure for the es- tablishment of maximum residue limits of veteri- nary medicinal products in oodstuffs of animal origin. Off J Eur Commun., L 224, 1-8.
  • Honkanen-Buzalski, T., Reybroeck, W., 1997. Antimicrobials. Monograph on Residues and Contaminants in Milk and Milk Products. IDF Special Issue 9701. International Dairy Federa- tion, Brussel: 26–33.
  • Huebra, M.J.G., Bordin, U.V.G., Rodriguez, A.R., 2005. Determination of macrolide antibiot- ics in porcine and bovine urine by high- performance liquid chromatography coupled to coulometric detection. Anal Bioanal Chem., 382: 433–439.
  • Huet, A.C, Fodey, T., Haughey, S.A., Weigel, S., Elliott, C., Delahaut, P., 2010. Advances in bio- sensor-based analysis for antimicrobial residues in foods. Trends Anal Chem., 29 (11), 1281- 1294.
  • Kaya, S.E., Filazi, A., 2010. Determination of Antibiotic Residues in Milk Samples. Kafkas Univ Vet Fak Derg., 16 (Suppl-A), S31-S35.
  • Mäyrä-Mäkinen , A., 1995. Technological signif- icance of residues for the dairy industry. In: Symposium on Residues of Antimicrobial Drugs and Other Inhibitors in Milk. IDF Special Issue No. 95 05. Kiel, Germany: 136–143.
  • Navrátilová, P., 2008. Screening methods used for the detection of veterinary drug residues in raw cow milk – a review. Czech J Food Sci., 26, 393–401.
  • Omeiza, G.K., Ajayi, I.E., Ode, O.J., 2012. Assesment of antimicrobial drug residues in beef in Abuja, the Federal Capital Territory. Vet Ital- iana, 48 (3), 283-289.
  • Oruç, H. H., Rumbeiha, W. K., Ensley, S., Wol- sen, C., Schrunk, D. E. Simultaneous Detection of Six Different Groups of Antimicrobial Drugs in Porcine Oral Fluids Using a Biochip Array- Based Immunoassay. Kafkas Univ Vet Fak Derg., (DOI: 10.9775/kvfd.2012.7905).
  • Oruç, H.H., Cengiz, M., Bağdaş, D., Uzunoğlu, İ., 2006. Sığır Etlerinde Streptomisin ve Sulfa- metazin (Sulfadimidin) Kalıntıları (Streptomycin and Sulfamethazin (Sulphadimidine) Residues in Meat). Uludag Univ J Fac Vet Med., 26 (1-2), 17-20.
  • Özcan, A., Karaca, M.Y., Bayraktar, D., 2011. Güney Marmara Bölgesinde Satışa Sunulan Hayvan Yemlerindeki Antibiyotik ve Antikoksid- iostat Düzeylerinin LC-MS/MS Yöntemi İle Be- lirlenmesi Üzerine Bir Araştırma. Gıda Yem Bil Tek Derg., 8(11),1-15.
  • Randox Manual, 2011. Antimicrobial Array II (AM II), EV 3524 Manual.
Toplam 15 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Bölüm Makaleler
Yazarlar

Hasan H. Oruç Bu kişi benim

Wilson K. Rumbeıha Bu kişi benim

Steve Ensley Bu kişi benim

Dwayne E. Schrunk Bu kişi benim

Yayımlanma Tarihi 1 Haziran 2012
Yayımlandığı Sayı Yıl 2012 Cilt: 31 Sayı: 2

Kaynak Göster

APA Oruç, H. H., Rumbeıha, W. K., Ensley, S., Schrunk, D. E. (2012). Simultaneous Detection of Six Different Groups of Antimicrobial Drugs in Milk, Meat, Urine, and Feed Matrices. Uludağ Üniversitesi Veteriner Fakültesi Dergisi, 31(2), 29-33.
AMA Oruç HH, Rumbeıha WK, Ensley S, Schrunk DE. Simultaneous Detection of Six Different Groups of Antimicrobial Drugs in Milk, Meat, Urine, and Feed Matrices. Uludağ Üniversitesi Veteriner Fakültesi Dergisi. Haziran 2012;31(2):29-33.
Chicago Oruç, Hasan H., Wilson K. Rumbeıha, Steve Ensley, ve Dwayne E. Schrunk. “Simultaneous Detection of Six Different Groups of Antimicrobial Drugs in Milk, Meat, Urine, and Feed Matrices”. Uludağ Üniversitesi Veteriner Fakültesi Dergisi 31, sy. 2 (Haziran 2012): 29-33.
EndNote Oruç HH, Rumbeıha WK, Ensley S, Schrunk DE (01 Haziran 2012) Simultaneous Detection of Six Different Groups of Antimicrobial Drugs in Milk, Meat, Urine, and Feed Matrices. Uludağ Üniversitesi Veteriner Fakültesi Dergisi 31 2 29–33.
IEEE H. H. Oruç, W. K. Rumbeıha, S. Ensley, ve D. E. Schrunk, “Simultaneous Detection of Six Different Groups of Antimicrobial Drugs in Milk, Meat, Urine, and Feed Matrices”, Uludağ Üniversitesi Veteriner Fakültesi Dergisi, c. 31, sy. 2, ss. 29–33, 2012.
ISNAD Oruç, Hasan H. vd. “Simultaneous Detection of Six Different Groups of Antimicrobial Drugs in Milk, Meat, Urine, and Feed Matrices”. Uludağ Üniversitesi Veteriner Fakültesi Dergisi 31/2 (Haziran 2012), 29-33.
JAMA Oruç HH, Rumbeıha WK, Ensley S, Schrunk DE. Simultaneous Detection of Six Different Groups of Antimicrobial Drugs in Milk, Meat, Urine, and Feed Matrices. Uludağ Üniversitesi Veteriner Fakültesi Dergisi. 2012;31:29–33.
MLA Oruç, Hasan H. vd. “Simultaneous Detection of Six Different Groups of Antimicrobial Drugs in Milk, Meat, Urine, and Feed Matrices”. Uludağ Üniversitesi Veteriner Fakültesi Dergisi, c. 31, sy. 2, 2012, ss. 29-33.
Vancouver Oruç HH, Rumbeıha WK, Ensley S, Schrunk DE. Simultaneous Detection of Six Different Groups of Antimicrobial Drugs in Milk, Meat, Urine, and Feed Matrices. Uludağ Üniversitesi Veteriner Fakültesi Dergisi. 2012;31(2):29-33.