In Vitro Evaluation of the Antineoplastic Activity of Silver Nanoparticles Functionalized with Bioactive Molecules Against SK-MEL-30, MCF-7, and H1299 Cancer Cells

Esra Bozkaya

doi:10.29137/ijerad.1674277

In Vitro Evaluation of the Antineoplastic Activity of Silver Nanoparticles Functionalized with Bioactive Molecules Against SK-MEL-30, MCF-7, and H1299 Cancer Cells

Abstract

The present study aimed to evaluate the in vitro antineoplastic activity of silver nanoparticles (AgNPs) biosynthesized using Centella asiatica (CA) leaf extract against human SK-MEL-30 skin cancer, MCF-7 breast cancer, and H1299 lung cancer cell lines. CA/AgNPs were synthesized via a green chemistry approach and characterized by standard physicochemical techniques. The anticancer potential of the synthesized nanoparticles was assessed using MTT assays, along with apoptotic and necrotic index evaluations. Results indicated a dose-dependent reduction in cell viability in all cancer cell lines, while minimal cytotoxicity was observed in normal L929 fibroblast cells. Notably, CA and CA/AgNPs induced apoptosis and necrosis selectively in cancer cells, suggesting that both formulations possess strong antitumoral effects. The apoptotic indices were especially high in H1299 lung cancer cells treated with CA extract, whereas necrosis was most pronounced in SK-MEL-30 cells at higher concentrations. Taken together, these findings support the antineoplastic, anticancer, and cytotoxic potential of CA/AgNPs, highlighting their promise as biocompatible agents for targeted cancer therapies. Further in vivo studies are recommended to validate these effects.

Keywords

Antineoplastic activity, silver nanoparticle, Centella asiatica, anticancer activity, apoptosis induction, green synthesis

Thanks

The author is grateful to Kırıkkale University and Kırıkkale University Scientific and Technological Researches Application and Research Center (KUBTUAM) for their support.

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Details

Primary Language

English

Subjects

Active Matter Design, Biomaterial , Animal Cell Culture and Tissue Engineering

Journal Section

Research Article

Authors

Esra Bozkaya ^*
0000-0002-9259-2538
Türkiye

Early Pub Date

November 23, 2025

Publication Date

November 30, 2025

Submission Date

April 11, 2025

Acceptance Date

October 15, 2025

Published in Issue

Year 2025 Volume: 17 Number: 3

DOI

https://doi.org/10.29137/ijerad.1674277

IZ

https://izlik.org/JA34WP76JD

APA

Bozkaya, E. (2025). In Vitro Evaluation of the Antineoplastic Activity of Silver Nanoparticles Functionalized with Bioactive Molecules Against SK-MEL-30, MCF-7, and H1299 Cancer Cells. International Journal of Engineering Research and Development, 17(3), 590-603. https://doi.org/10.29137/ijerad.1674277

AMA

1.Bozkaya E. In Vitro Evaluation of the Antineoplastic Activity of Silver Nanoparticles Functionalized with Bioactive Molecules Against SK-MEL-30, MCF-7, and H1299 Cancer Cells. IJERAD. 2025;17(3):590-603. doi:10.29137/ijerad.1674277

Chicago

Bozkaya, Esra. 2025. “In Vitro Evaluation of the Antineoplastic Activity of Silver Nanoparticles Functionalized With Bioactive Molecules Against SK-MEL-30, MCF-7, and H1299 Cancer Cells”. International Journal of Engineering Research and Development 17 (3): 590-603. https://doi.org/10.29137/ijerad.1674277.

EndNote

Bozkaya E (November 1, 2025) In Vitro Evaluation of the Antineoplastic Activity of Silver Nanoparticles Functionalized with Bioactive Molecules Against SK-MEL-30, MCF-7, and H1299 Cancer Cells. International Journal of Engineering Research and Development 17 3 590–603.

IEEE

[1]E. Bozkaya, “In Vitro Evaluation of the Antineoplastic Activity of Silver Nanoparticles Functionalized with Bioactive Molecules Against SK-MEL-30, MCF-7, and H1299 Cancer Cells”, IJERAD, vol. 17, no. 3, pp. 590–603, Nov. 2025, doi: 10.29137/ijerad.1674277.

ISNAD

Bozkaya, Esra. “In Vitro Evaluation of the Antineoplastic Activity of Silver Nanoparticles Functionalized With Bioactive Molecules Against SK-MEL-30, MCF-7, and H1299 Cancer Cells”. International Journal of Engineering Research and Development 17/3 (November 1, 2025): 590-603. https://doi.org/10.29137/ijerad.1674277.

JAMA

1.Bozkaya E. In Vitro Evaluation of the Antineoplastic Activity of Silver Nanoparticles Functionalized with Bioactive Molecules Against SK-MEL-30, MCF-7, and H1299 Cancer Cells. IJERAD. 2025;17:590–603.

MLA

Bozkaya, Esra. “In Vitro Evaluation of the Antineoplastic Activity of Silver Nanoparticles Functionalized With Bioactive Molecules Against SK-MEL-30, MCF-7, and H1299 Cancer Cells”. International Journal of Engineering Research and Development, vol. 17, no. 3, Nov. 2025, pp. 590-03, doi:10.29137/ijerad.1674277.

Vancouver

1.Esra Bozkaya. In Vitro Evaluation of the Antineoplastic Activity of Silver Nanoparticles Functionalized with Bioactive Molecules Against SK-MEL-30, MCF-7, and H1299 Cancer Cells. IJERAD. 2025 Nov. 1;17(3):590-603. doi:10.29137/ijerad.1674277

Kırıkkale University, Faculty of Engineering and Natural Science, 71450 Yahşihan / Kırıkkale, Türkiye.

ijerad@kku.edu.tr

In Vitro Evaluation of the Antineoplastic Activity of Silver Nanoparticles Functionalized with Bioactive Molecules Against SK-MEL-30, MCF-7, and H1299 Cancer Cells

Abstract

Keywords

Thanks

References

Details

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Subjects

Journal Section

Authors

Early Pub Date

Publication Date

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Acceptance Date

Published in Issue

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