Yıl 2019, Cilt 11 , Sayı 2, Sayfalar 643 - 656 2019-06-30

Katkısız ve Gümüş Katkılı Hidroksiapatitin Fiziksel ve Kimyasal Karakterizasyonu
Physical and Chemical Characterization of Pure and Silver Doped Hydroxyapatite

Mustafa Burak TÜRKÖZ [1] , Ümit ERDEM [2]

Bu çalışmada çöktürme yöntemiyle nano boyutlu katkısız Hidroksiapatit (HAp) ve %0,05, %0,20, %0,40 (w/v) oranlarında Ag(I) iyonu katkılanmış HAp örnekleri üretilerek, bu örneklerin fiziksel ve kimyasal karakterizasyonları yapılmıştır. Kimyasal yapı ve kristalografik incelemede XRD, yüzey morfolojisi incelemesinde SEM, elementel analizde SEM-EDS ve ICP-OES, tane boyut incelemesinde Zeta Sizer, bileşen gruplarının belirlenmesinde FTIR, yoğunluk ölçümü için Archimed yöntemleri kullanılmıştır. XRD sonuçlarına göre, katkısız HAp için 2θ=31,77 olarak belirlenmiştir. Katkı oranlarındaki değişimin yüzey morfolojisine net bir etkisinin olmadığı SEM sonuçlarından elde edilmiştir. EDS ile Ca/P oranı katkısız HAp için 1,668 olarak bulunmuştur. SEM ve Zeta Sizer ölçümlerinden 100-500 nm aralığında tane boyutları elde edilmiştir. Üretilen örnekler için yoğunluk değeri 3,051 - 3,140 gr/cm3 olarak ölçülmüştür. Tüm bu sonuçların ışığında, üretimi yapılan katkısız ve Ag(I) katkılı örnekler literatürle uyumlu ve biyolojik çalışmalarda kullanılabilecek özelliklerde olduğu anlaşılmaktadır.

In this study, nano-sized pure HAp and 0.05%, 0.20%, 0.40% (w / v) Ag (I) ion-doped HAp samples were produced by precipitation method also physical and chemical characterization of these samples were made. According to the XRD results, 2θ= 31.77° for pure HAp. No significant changes were observed in the 2θ values ​​according to the silver dopping rate. From the SEM images, it was observed that the change in the dopping rates did not have a clear effect on the surface morphology. The Ca / P ratio with the SEM were 1.6685 for HAp. The Ca / P ratio for pure HAp in ICP-OES device was found to be 1.6630. Grain size mean values ​​of samples were measured 100-400 nm. FTIR results were consistent with the literature. From the produced samples, the density value for pure HAp was found to be 3,051 gr / cm3. It was observed that the densities of Ag (I) -HAp powders increased as the contribution ratio increased. In the light of all these results, it is understood that the pure and silver doped samples are compatible with the literature and can be used in biological studies.

  • Chu C H, Lo E C M, & Lin H C. Effectiveness of silver diamine fluoride and sodium fluoride varnish in arresting dentin caries in Chinese pre-school children. Journal of dental research,81(11), 767-770, 2002.
  • Llodra J C, Rodriguez A, Ferrer B,Menardia V, Ramos T, & Morato M. Efficacy of silver diamine fluoride for caries reduction in primary teeth and first permanent molars of schoolchildren: 36-month clinical trial. Journal of dental research, 84(8),721-724, 2005.
  • Tan H P, Lo E C M, Dyson J E, Luo Y, & Corbet E F. A randomized trial on root caries prevention in elders. Journal of dental research, 89(10), 1086-1090, 2010.
  • Zhang W, McGrath C, Lo E C.M, & Li J Y. Silver diamine fluoride and education to prevent and arrest root caries among community-dwelling elders. Caries research, 47(4), 284-290, 2013.
  • Earl, J., et al. Physical and chemical characterization of dentin surface following treatment with NovaMin technology. The Journal of clinical dentistry, 22(3): 62-67, 2011.
  • Arnold, W., et al. Effectiveness of various toothpastes on dentine tubule occlusion. Journal of Dentistry, 43(4): 440-449, 2015.
  • Gysi, A., An attempt to explain the sensitiveness of dentine., British Journal of Dental Science, 43: 865-868, 1990.
  • Byers, M. R. and W. K. Dong., Autoradiographic location of sensory nerve endings in dentin of monkey teeth., The Anatomical Record, 205(4): 441-454, 1983.
  • Hirvonen, T. and M. Narhi., The effect of dentinal stimulation on pulp nerve function and pulp morphology in the dog., Journal of dental research, 65(11): 1290-1293, 1986.
  • Brannstrom, M., & Åström, A., A study on the mechanism of pain elicited from the dentin. Journal of dental research, 43(4), 619-625, 1964.
  • Addy, M. and P. Mostafa. Dentine hypersensitivity. I. Effects produced by the uptake in vitro of metal ions, fluoride and formaldehyde onto dentine. Journal of Oral Rehabilitation, 15(6): 575-585, 1988.
  • Pashley, D. H., et al. Effects of the degree of tubule occlusion on the permeability of human dentine in vitro. Archives of oral biology, 23(12): 1133, 1978.
  • Lee, S., et al. (2008). "Effect of dentinal tubule occlusion by dentifrice containing nano‐carbonate apatite." Journal of oral rehabilitation, 35(11): 847-853.
  • Lavender, S. A., et al. (2010). Mode of action studies of a new desensitizing dentifrice containing 8.0% arginine, a high cleaning calcium carbonate system and 1450 ppm fluoride. American journal of dentistry, 23(Special Issue): 14A-19A.
  • Cummins, D. (2011). Advances in the clinical management of dentin hypersensitivity: a review of recent evidence for the efficacy of dentifrices in providing instant and lasting relief. Journal of Clinical Dentistry, 22(4): 100.
  • Cunha-Cruz, J., et al. (2011). Dentin hypersensitivity and oxalates: a systematic review. Journal of Dental Research, 90(3): 304-310.
  • Wang, R., et al. (2014). Enhancement of nano-hydroxyapatite bonding to dentin through a collagen/calcium dual-affinitive peptide for dentinal tubule occlusion. Journal of biomaterials applications, 29(2): 268-277.
  • Chiang, Y.-C., et al. (2010). A novel mesoporous biomaterial for treating dentin hypersensitivity. Journal of dental research, 89(3): 236-240.
  • Vallet-Regi, M., "Ceramics for Medical Applications", The Royal Society of Chemistry, Dalton Transactions, pp. 97 – 108, 2000.
  • Kweh, S. W. K. et al., The Production and Characterisation of Hydroxyapatite (HA) Powder", Journal of Materials Processing Technologies, Vol. 89-90, pp. 373 – 377, 1999.
  • Turkoz, M., Atilla, A. O., & Evis, Z.. Silver and fluoride doped hydroxyapatites: investigation by microstructure, mechanical and antibacterial properties. Ceramics International, 39(8), 8925-8931, 2013.
  • McLaughlin, M. et al. Particle Size Analysis- When to use the Fraunhofer Approximation versus the Mie Theory‖, PDS-Analytical Development, Patheon Inc., 2005.
  • Khan, P. K., Mahato, A., Kundu, B., Nandi, S. K., Mukherjee, P., Datta, S., ... & Mandal, C., Influence of single and binary doping of strontium and lithium on in vivo biological properties of bioactive glass scaffolds. Scientific reports, 6, 32964, 2016.
  • Slosarczyk, A. et al., "FTIR and XRD Evaluation of Carbonated Hydroxyapatite Powders Synthesised by Wet Methods", Journal of Molecular Structure, Volumes 744-747, pp. 657 – 661, 2005.
  • Destainville, A., Champion, E., Bernache-Assollant, D., & Laborde, E. Synthesis, characterization and thermal behavior of apatitic tricalcium phosphate. Materials Chemistry and Physics, 80(1), 269-277, 2003.
  • ISO 13779, Part 1, Implants for Surgery – Hydroxyapatite-Part 1: Ceramic Hydroxyapatite‖, International Standards Organisation, 2000.
  • ASTM F1185, Standard Specification for Composition of Hydroxylapatite for Surgical Implants‖, American Society for Testing and Materials, 2003.
  • ASTM E2149 - 13a Standard Test Method for Determining the Antimicrobial Activity of Antimicrobial Agents Under Dynamic Contact Conditions.
  • U.S. Food and Drug Administration, "Calcium Phosphate (Ca-P) Coating Draft Guidance for Preparation of FDA Submissions for Orthopaedic and Dental Endosseous Implants", 1992.
  • Puajindanetr, S.,Characterisation and Sintering of Precipitated Hydroxyapatite, Ph.D. Thesis, Queen Mary and Westfield College London, Great Britain, 1993.
  • Smiciklas, I. et al. "Experimental Design Approach in the Synthesis of Hydroxyapatite by Neutralization Method", Separation and Purification Technology, Volume 44, Issue 2, pp. 97 – 102, 2005.
  • Hellmich, C. and Ulm, F. J., (2002), ―Micromechanical Model for Ultrastructural Stiffness of Mineralized Tissues‖, Journal of Engineering Mech., Vol. 128, Issue 8, pp. 898 – 908.
  • Keskin, A., Hidroksiapatit seramiklerinin mekanik özelliklerinin zirkonya ilavesi ile geliştirilmesi, Yüksek Lisans Tezi, İTÜ, Fen Bilimleri Enstitüsü, İstanbul, 2000.
  • Giuca MR, Cei G, Gigli F, Gandini P. "Oral signs in the diagnosis of celiac disease: review of the literature". Minerva Stomatol (Review). 59 (1–2): 33–43, 2010.
  • Touger-Decker R, van Loveren C., "Sugars and dental caries". Am. J. Clin. Nutr. 78(4): 881S–892S, 2003.
  • Hardie JM., "The microbiology of dental caries". Dent Update. 9 (4): 199–200, 202–4, 206–8. 1982.
  • Verron E, Khairoun I, Guicheux J, Bouler J. Calcium phosphate biomaterials as bone drug delivery systems: a review. Drug Discov Today. 2010;15(13–14):547-552.
  • Seckler, M. M. et al., "Influence of Process Conditions on Hydroxyapatite Crystallinity Obtained by Direct Crystallization", Materials Research, Vol. 2, No. 2, pp. 59 – 62, 1999.
  • Giulietti, M. et al., "Industrial Crystallisation and Precipitation from Solutions: State of the Technique", Brazilian Journal of Chemical Engineering, Vol. 15, Issue 4, pp. 423 – 440, 2001.
  • Lazic, S. et al., "Properties of Hydroxyapatite Crystallized from High Temperature Alkaline Solutions", Journal of Crystal Growth, (165),124 – 128, 1996.
  • Lazic, S. et al., "The effect of Temperature on the Properties of Hydroxyapatite Precipitated from Calcium Hydroxide and Phosphoric Acid", Thermochimica Acta, Vol. 374, pp. 13 – 22, 2001.
  • Dorozhkin SV. Calcium Orthophosphates as Bioceramics: State of the Art. Journal of Functional Biomaterials. 2010;1(1):22-107.Thomas M, Ghosh S, George K. Characterization of nano-structured silver orthophosphate. Materials Letters.,56(4):386-392,2002.
Birincil Dil tr
Konular Mühendislik, Ortak Disiplinler
Bölüm Makaleler

Orcid: 0000-0002-4127-7650
Yazar: Mustafa Burak TÜRKÖZ (Sorumlu Yazar)
Ülke: Turkey

Orcid: 0000-0002-0480-8176
Yazar: Ümit ERDEM
Ülke: Turkey


Yayımlanma Tarihi : 30 Haziran 2019

APA Türköz, M , Erdem, Ü . (2019). Katkısız ve Gümüş Katkılı Hidroksiapatitin Fiziksel ve Kimyasal Karakterizasyonu . International Journal of Engineering Research and Development , 11 (2) , 643-656 . DOI: 10.29137/umagd.539364