Cerrahi sütür uygulamaları için poli(hegzametilen biguanid) immobilize edilmiş emilmeyen ve antimikrobiyal PET fiber: sentez, karakterizasyon ve in vitro sitouyumluluk değerlendirmesi

Yıl 2024, Cilt: 16 Sayı: 2, 778 - 791, 30.06.2024

Ogün Bozkaya Kübra Günay Esra Bozkaya Metin Arslan

https://doi.org/10.29137/umagd.1473795

Öz

Cerrahi sütürler, cerrahi müdahalelerden sonra yaraları kapatmak amacıyla yaygın olarak kullanılan tıbbi cihazlardır. Cerrahi prosedürler sırasında, sütürler çevredeki mikroorganizmalarla temas ederek bakteriyel biyofilmlerin gelişmesine ve ardından cerrahi alan enfeksiyonlarına (CAE) yol açabilir. Bu nedenle bakteri tutunmasını ve kolonizasyonunu engelleyen sütürlerin kullanılması yara iyileşmesinde oldukça önemlidir. CAE oluşumunu en aza indirmek için triklosan ve klorheksidin gibi antiseptiklerle kaplı antimikrobiyal sütürler kullanılmıştır. Ancak çeşitli antiseptiklere ve antibiyotiklere dirençli bakteri suşlarının ortaya çıkması nedeniyle alternatif antimikrobiyal sütürlerin geliştirilmesine yönelik yeni yaklaşımlar gereklidir. Antimikrobiyal ajanlar arasında, poliheksanid (poliheksametilen biguanid, PHMB) geniş spektrumlu antibakteriyel ve antifungal aktiviteye sahip, düşük toksisiteli ve biyouyumlu katyonik bir antiseptiktir. Bu çalışmada cerrahi dikiş ipliği olarak kullanılmak üzere PHMB immobilize edilmiş polietilen tereftalat (PET) liflerin sentezlenmesi amaçlanmıştır. Sentezlenen modifiye PET lifler SEM, FTIR, DSC ve TGA ile karakterize edilmiştir. Bunlara ek olarak modifier PET liflerin ıslanabirliği ve in vitro degradasyon profili araştırılmıştır. Antimikrobiyal etkinlik testleri için S. aureus ve E. coli bakteri suşları kullanılmıştır. Materyallerin in vitro hücre-uyumluluğu (sitotoksisitesi) TS EN ISO 10993-5 standardına göre MTT testi ile değerlendirilmiştir.

Anahtar Kelimeler

Cerrahi sütür, Emilemez sütür”, Antimikrobiyal sütür, Polihegzametilen biguanid, Poliheksanid, Polietilen tereftalat

Proje Numarası

2022/0041

Poly(hexamethylene biguanide) immobilized non-absorbable and antimicrobial PET fiber for surgical suture applications: synthesis, characterization and in vitro cytocompatibility assessment

Öz

Surgical sutures are widely utilized medical devices for the purpose of closing wounds after surgical interventions. During surgical procedures, sutures can come into contact with microorganisms in the surrounding environment, leading to the development of bacterial biofilms and subsequent surgical site infections (SSI). Therefore, the use of sutures that prevent bacterial attachment and colonization is very important in wound healing. Antimicrobial sutures coated with antiseptics such as triclosan and chlorhexidine have been used to minimize SSI formation. However, due to the emergence of bacterial strains resistant to various antiseptics and antibiotics, new approaches to the development of alternative antimicrobial sutures are required. Among antimicrobial agents, polyhexanide (polyhexamethylene biguanide, PHMB) is a cationic antiseptic with broad spectrum antibacterial and antifungal activity, low toxicity and biocompatible. The aim of this study was to synthesize PHMB immobilized polyethylene terephthalate (PET) fibers for use as surgical suture threads. The synthesized modified PET fibers were characterized by SEM, FTIR, DSC and TGA. In addition, the wettability and in vitro degradation profile of the modified PET fibers were investigated. S. aureus and E. coli bacterial strains were used for antimicrobial activity tests. In vitro cytocompatibility (cytotoxicity) of the materials was evaluated by MTT test according to TS EN ISO 10993-5 standard.

Anahtar Kelimeler

Surgical suture, Antimicrobial suture, Non-adsorbable suture, Polyhexamethylene biguanide, Polyhexanide, Polyethylene terephthalate

Destekleyen Kurum

Kırıkkale University Scientific Commite

Proje Numarası

2022/0041

Teşekkür

The authors would like to thank Kırıkkale University Scientific and Technological Research Application and Research Centre (KUBTUAM).

Kaynakça

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