Farklı Remineralize Edici Maddeler İçeren Ağartma Ajanlarının Sığır Diş Mine Yüzey Özellikleri Üzerine Etkilerinin Değerlendirilmesi

Öz

Bu çalışmanın amacı farklı remineralize edici maddeler içeren ağartma ajanlarının sığır dişi minesinin mineral içeriği üzerine etkilerinin SEM-EDX yöntemi ile değerlendirilmesidir. Bu çalışma 32 adet sığır dişinden elde edilen mine örnekleri üzerinde gerçekleştirildi. Örnekler rastgele 4 gruba ayrıldı. Grup1: %35’lik hidrojen peroksit (HP), Grup2: %35’lik hidrojen peroksit+kalsiyum klorür (CaCl2), Grup3: %35’lik hidrojen peroksit+amorf kalsiyum fosfat (ACP), Grup4: %35’lik hidrojen peroksit+kalsiyum (Ca) içeren ağartma ajanı uygulandı. Her gruptaki mine örnekleri iki eş parçaya ayrıldı. Bir parçasına beyazlatma öncesi, diğer parçasına beyazlatma sonrası SEM-EDX analizi yapıldı. Kalsiyum, fosfat ve kalsiyum/fosfat değerleri kaydedildi. Değişkenlerin normal dağılıp dağılmadığı Kolmogorov-Smirnow ve Shapiro-Wilk uyum testiyle araştırılmış ve verilerin normal dağıldıkları görülmüştür. Gruplar arası karşılaştırmalarda Paired Samples t-testi kullanılmıştır. SEM-EDX analizinin sonuçlarına göre hidrojen peroksit içeren ağartma ajanı ile içerisine remineralize edici maddeler ilave edilen hidrojen peroksit ağartma ajanı arasında kalsiyum, fosfat ve kalsiyum/fosfat oranı açısından anlamlı bir farklılık bulundu (p<0,05). Ağartma ajanları içerisine remineralize edici maddeler eklenmesi ağartma sırasında oluşabilecek diş mine yüzey demineralizasyonunu engellediği sonucuna varıldı.

Anahtar Kelimeler

• Ağartma
• remineralizasyon
• SEM-EDS

Assessment Of The Effects Of Bleachıng Agents Contaınıng Dıfferent Remıneralısıng Materıals On Bovıne Teeth Enamel Surface Propertıes

Öz

The aim of this study was to evaluate the effects of bleaching agents containing different remineralizing agents on mineral content of bovine enamel by SEM-EDX method. This study was conducted on enamel samples obtained from 32 bovine teeth. The samples were randomly divided into 4 groups. Group1: 35% hydrogen peroxide containing bleaching agent, Group2: 35% hydrogen peroxide + calcium chloride, Group3: 35% hydrogen peroxide + amorphous calcium phosphate, Group4: 35% hydrogen peroxide + calcium containing bleaching agent was applied. Enamel samples in each group were divided into two identical parts. SEM-EDX analysis was performed before bleaching on one part and after bleaching on the other part. Calcium, phosphate and calcium/phosphate values were recorded. Whether variables are normally distributed or not has been investigated with the Kolmogorov-Smirnov and Shapiro-Wilk compliance tests and the data were found to be normally distributed. Paired Samples t-test was used in comparisons between groups. According to the results of SEM-EDX analysis, a significant difference was found between the hydrogen peroxide bleaching agent and the hydrogen peroxide bleaching agent, in which remineralizing agents were added, in terms of calcium, phosphate and calcium/phosphate ratio (p <0.05). It was concluded that the addition of remineralizing agents to bleaching agents prevents the surface enamel demineralization during bleaching.

Anahtar Kelimeler

• Bleaching
• remineralisation
• SEMEDS

Kaynakça

1. 1. Borges, A.B., Zanatta, R., Barros, A., Silva, L., Pucci, C. and Torres C. Effect of hydrogen peroxide concentration on enamel color and microhardness. Oper Dent 2014;40(1):96-101.
2. 2. Cervantes, A., Bolanho, A., Valera, M.C. and Araújo, M.A. Microhardness study of bovine enamel to bleaching treatment activated for different sources of light. Braz Dent Sci. 2006;9:78-86.
3. 3. Giannini, M., Silva, A.P., Cavalli, V. and Paes Leme, A.F. Effect of carbamide peroxide-based bleaching agents containing fluoride or calcium on tensile strength of human enamel. J Appl Oral Sci 2006;14:82- 7.
4. 4. Tam, L. Vital tooth bleaching: Review and current status. JCDA. 1992;58:654-659.
5. 5. Attin, T., Schmidlin, P.R., Wegehaupt, F. and Wiegand, A. Influence of study design on the impact of bleaching agents on dental enamel microhardness: A review. Dent Mater. 2009;25(2):143-157.
6. 6. Soldani, P., Amaral, C.M., Rodrigues, J.A. Microhardness evaluation of in situ vital bleaching and thickening agents on human dental enamel. Int J Periodontics Restorative Dent. 2010;30:203-11.
7. 7. Akal, N., Over, H., Olmez, A., and Bodur, H. Effects of carbamide peroxide containing bleaching agents on the morphology and subsurface hardness of enamel. J Clin Pediatr Dent. 2001;25(4):293-296.
8. 8. Lewinstein, .I, Fuhrer, N., Churaru, N. and Cardash, H. Effect of different peroxide bleaching regimens and subsequent fluoridation on the hardness of human enamel and dentin. J Prosthet Dent. 2004;92:337-42.

9. 9. Pinto, C.F., Paes Leme, A.F., Cavalli, V. and Giannini, M. Effect of 10% carbamide peroxide bleaching on sound and artifi cial enamel carious lesions. Braz Dent J. 2009;20:48-53.
10. 10. Lia Mondelli, R. F., Garrido Gabriel, T. R. C., Piola Rizzante, F. A., Magalhães, A. C., Soares Bombonatti, J. F., and Ishikiriama, S. K. Do different bleaching protocols affect the enamel microhardness? Eur J Dent. 2015;1:25–30.
11. 11. Thylstrup, A., Bruun, C. and Holmen, L. In vivo caries models--mechanisms for caries initiation and arrestment. Adv Dent Res. 1994;8(2):144-57.
12. 12. White, D.J. The application of in vitro models to research on demineralization and remineralization of the teeth Adv Dent Res. 1995;9(3):175-193.
13. 13. Rodrigues, E., Delbem, A. C., Pedrini, D. and Cavassan, L. Enamel remineralization by fluo-ride-releasing materials: proposal of a pH-cycling model. Braz Dent J. 2010;21(5):446-51.
14. 14. Tang, G., Yip, H.K., Cutressc, T.V. and Samaranayakea, L.P. Artificial mouth model systems and their contribution to caries research. J Dent. 2003;31:161–171.
15. 15. Vidhya, S., Srinivasulu, S., Sujatha, M. and Mahalaxmi, S. Effect of grape seed extract on the bond strength of bleached enamel. Oper Dent. 2011;4:433-438.
16. 16. Khoroushi, M. and Aghelinejad, S. Effect of postbleaching application of anantioxidant on enamel bond strength of three different adhesives. Med Oral Patol Oral Cir Bucal. 2011;7:990-996.
17. 17. Scougall-Vilchis, R.J., Gonzalez-Lopez, B.S., Contreras-Bulnes, R., Rodriguez- Vilchis, L.E., Garcia-Nino de Rivera, M.W.and Kubodera-Ito, T. Influence of four systems for dental bleaching on the bond strength of orthodontic brackets. Angle Orthod. 2011;4:700-706.
18. 18. Titley, K. et al. The effect of concentrated hydrogen peroxide on the surface morphology of human tooth enamel. J Endod. 1988;14(2):69-74.
19. 19. Cakir, F.Y., Korkmaz, Y., Firat, E., Oztas, S.S., and Gurgan, S. Chemical analysis of enamel and dentin following the application of three different athome bleaching systems. Oper Dent. 2011;5:529-536.
20. 20. Borges, A.B., Torres, C.R., de Souza, P.A., Caneppele, T.M., Santos, L.F. and Magalhães, A.C. Bleaching gels containing calcium and fluoride: effect on enamel erosion susceptibility. Int J Dent. 2012;347848.
21. 21. Hegde, M. N., Moany, A. Remineralization of enamel subsurface lesions with casein phosphopeptide-amorphous calcium phosphate: A quantitative energy dispersive X-ray analysis using scanning electron microscopy: An in vitro study. J Conserv Dent. 2012;1:61–67.
22. 22. Sda, S. Et Al. “the effect of fluoride therapies on the morphology of bleached human dental enamel ferreira. Microsc Res Tech. 2011;74(6):512-516.
23. 23. Hegde, M.N., Shetty, S. and Pardal, D. Remineralization of enamel sub-surface lesion using casein phosphopeptideamorphous calcium phosphate. J Conserv Dent. 2007;10:19–25.
24. 24. Bakry, A.S., Marghalani, H.Y., Amin, O.A. and Tagami, J. The effect of a bioglass paste on enamel exposed to erosive challenge. J Dent. 2014;42(11):1458-1463.
25. 25. Alexandria, A.K., Nassur, C., Nóbrega, C.B.C., Valença, A.M.G., Rosalen, P.L. and Maia, L.C. In situ effect of titanium tetrafluoride varnish on enamel demineralization. Braz Oral Res. 2017;6(31):e86.
26. 26. Danesh-Sani, S.A., Esmaili, M. Effect of 10% sodiuascorbate hydrogel and delayed bonding on shear bond strenght of composite resin anda resinmodified glass ionomer to bleached enamel. J Conserv Dent. 2011;3:241-6
27. 27. Esmaili, M Danesh-Sani, S.A.,.M ascorbate hydrogel and delayed bonding on shear bond strength of composite resin and resinmodified glass ionomer to bleached enamel. J Conserv Dent. 2011;3:241-246.
28. 28. Rass, M.A. Long-term prognosis ofinternational endodontics and biternal bleaching of tetracycline-stained teeth. Compendum, 1998;10:1034-1050.
29. 29. Mangal, T. Vital ve devital yapılmış diş yüzeylerine uygulanan farklı yüzey tedavilerinin tekrar renklenmeye ve çürük yapıcı mikrooraganizmaların tutunmasına etkileri. Doktora Tezi, Gazi Üniversitesi Sağlık Bilimleri Enstitüsü, Ankara, 2014:10-22.
30. 30. Walstra, P., Geurts, T. J., Noomen, A., Jellema, A., Boekel, van, MAJS. Dairy technology: principles of milk properties and processes. New York: Marcel Dekker,Inc. 1999.
31. 31. Kumar, V.L., Itthagarun, A. and King, N.M. The effect of casein phosphopeptideamorphous calcium phosphate on remineralization of artificial caries-like lesions: an in vitro study. Aust Dent J. 2008;1:34-40.
32. 32. Sasaki, R.T., Catelan, A., Bertoldo Edos, S., Venâncio, P.C., Groppo, F.C., Ambrosano, G.M., Marchi, G.M., Lima, D.A. and Aguiar, F.H. Effect of 7.5% hydrogen peroxide containing remineralizing agents on hardness, color change, roughness and micromorphology of human enamel. J Am Dent Assoc. 2015;5:261-7.
33. 33. Borges, A. B., Samezima, L. Y., Fonseca, L. P., Yui, K. C. K., Borges, A. L. S., & Torres, C. R. G. Influence of potentially remineralizing agents on bleached enamel microhardness. Oper Dent. 2009;34(5):593-597.
34. 34. Al-Salehi, S. K., Wood, D. J. And Hatton, P. V. The effect of 24 hour non-stop hydrogen peroxide concentration on bovine enamel and dentine mineral content and microhardness. J Dent. 2007;35, (11):845– 850.
35. 35. Tezel, H., Ertas O.S., Ozata F., Dalgar H., and Korkut, Z.O. Effect of bleaching agents on calcium loss from the enamel surface. Quintessence Int. 2007;38 (4):339–347.
36. 36. Zhang, Y. P., Din C. S., Miller S., Nathoo S.A., and A. Gaffar. Intra-oral remineralization of enamel with a MFP/DCPD and MFP/silica dentifrice using surface microhardness. J Clin Dent.1995;6(2):148–153.
37. 37. Attin, T., Kocabiyik M., Buchalla W., Hannig C., and Becker K. Susceptibility of enamel surfaces to demineralization after application of fluoridated carbamide peroxide gels. Caries Res. 2003;37(2):93– 99.
38. 38. Hughes, J. A., West, N. X. Parker, D. M. van den Braak, M. H. and Addy, M. Effects of pH and concentration of citric, malic and lactic acids on enamel, in vitro. J Dent. 2000;28(2):147–152.
39. 39. De Oliveira, R., Paes Leme, A. F., and Giannini, M. Effect of a carbamide peroxide bleaching gel containing calcium or fluoride on human enamel surface microhardness. Braz. Dent. J. 2004;16(2):103–106.