Effect of subacute malathion application on oxidative stress biomarkers

Abstract

In this study, it was aimed to investigate the effects of malathion on oxidative stress biomarkers and liver enzymes and the protection of caffeic acid phenethyl ester against malathione. In the study, a total of 40 (200-240 g) adult male Spraque Dawley rats with 10 animals in each group were used. In the study, 5 ml / kg corn oil by gavage in the control (C) group, 40 mg / kg malathion by gavage in the malathion (MAL) group, CAPE (10 µmol / kg) intraperitoneally in the malathion + caffeic acid phenethyl ester (MAL + CAPE) group. After 1 hour, malathion (40 mg / kg) was administered by gavage, and CAPE (10 µmol / kg) was administered intraperitoneally to the caffeic acid phenethyl ester (CAPE) group. At the end of 15 days of application, the blood of the rats was intracardiac under ketamine / xylazine anesthesia and the animals were applied cervical dislocation method. Paraoxonase (PON), aspartate aminotransferase (AST), alanine aminotransferase (ALT) activities and high density lipoprotein (HDL), malondialdehyde (MDA) and nitric oxide (NO) levels were analyzed in plasma obtained from blood samples. When the analysis results were evaluated, plasma PON activity and HDL levels decreased (P<0.01) in MAL and MAL+CAPE groups compared to the control group, while AST-ALT activities (P<0.05), MDA and NO levels increased (p<0.01) was detected. According to the results obtained from the study, it was determined that malathion caused significant changes in oxidative stress biomarkers, while CAPE could prevent these changes and be protective against malathion toxicity

Keywords

• Caffeic acid phenethyl ester
• nitric oxide
• oxidative stress
• paraoxonase
• malathion

Subakut malathion uygulamasının oksidatif stres biyobelirteçlerine etkisi

Öz

Bu çalışmada malathionun oksidatif stres biyobelirteçleri ve karaciğer enzimleri üzerine etkileri ile kafeik asit fenetil esterin malathiona karşı koruyuculuğunun araştırılması amaçlandı. Çalışmada her grupta 10 adet hayvan olacak şekilde toplam 40 adet (200-240 g) erişkin erkek Spraque Dawley ırkı rat kullanıldı. Çalışmada kontrol (K) grubuna gavaj yoluyla 5 ml/kg mısır yağı, malathion (MAL) grubuna gavaj yoluyla 40 mg/kg malathion, malathion+kafeik asit fenetil ester (MAL+CAPE) grubuna intraperitoneal yolla CAPE (10 µmol/kg) ve 1 saat sonra gavaj yoluyla malathion (40 mg/kg), kafeik asit fenetil ester (CAPE) grubuna ise intraperitoneal yolla CAPE (10 µmol/kg) uygulandı. 15 günlük uygulama sonunda ratların ketamin/ksilazin anestezi altında intrakardiyak olarak kanları alındı ve hayvanlara servikal dislokasyon yöntemi uygulandı. Alınan kan örneklerinden elde edilen plazmada paraoksonaz (PON), aspartat aminotransferaz (AST), alanin aminotransferaz (ALT) aktiviteleri ile yüksek dansiteli lipoprotein (HDL), malondialdehit (MDA) ve nitrik oksit (NO) düzeyleri analiz edildi. Analiz sonuçları değerlendirildiğinde, kontrol grubuna göre MAL ve MAL+CAPE gruplarında plazma PON aktivitesi ve HDL düzeylerinde azalma (P<0.01) olduğu, AST-ALT aktivitelerinde (P<0,05) MDA ve NO düzeylerinde ise (p<0.01) artış olduğu tespit edildi. Çalışmadan elde edilen sonuçlara göre, malathionun oksidatif stres biyobelirteçlerinde önemli değişiklikler meydana getirdiği, CAPE’nin ise bu değişiklikleri önleyebileceği ve malathion toksikasyonuna karşı koruyucu olabileceği belirlendi.

Anahtar Kelimeler

• Kafeik asit fenetil ester
• malathion
• nitrik oksit
• oksidatif stres
• paraoksonaz

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