Multilokus Variable Number of Tandem Repeat Analiz (MLVA) Veri Tabanlarında Türkiye'den İzole Edilen Bacillus anthracis Genotiplerinin Moleküler Epidemiyolojisi

Öz

Antraks, dünya genelinde insan ve hayvan sağlığını tehdit eden Bacillus anthracis’in neden olduğu önemli bir hastalıktır. B. anthracis doğada uzun süreler boyunca spor halinde kalması nedeniyle genetik olarak homojen bir yapı göstermektedir. Ancak epidemiyolojik olarak multiple-locus variable-number tandem repeat analysis (MLVA), single nucleotide repeat (SNR), genome-wide single nucleotide polymorphism (SNP) gibi moleküler metotlar ile suşlar arasında ayrım yapılabilmektedir. Bu araştırmada, MLVA Bank for Genotyping Microbes veri tabanına yüklenmiş olan B. anthracis MLVA-8 genotiplerinin analiz edilmesi amaçlandı. Bu araştırmada veri tabanında bulunan 49 ülkeye ait 2313 genotip profili değerlendirildi. Toplamda 402 genotip, 24 klonal grup içerisinde dağılım gösterdi. Türkiye’ye ait genotipler ise, 8 grup içerisinde dağılım gösterdi. Türkiye genotiplerinden en yüksek sayıdaki genotip (%31) XXIV klonal grubunda yerleşim gösterdi. Analiz sonucunda tespit edilen genotiplerin %63,2’si IX, XXIII ve XXIV klonal grupları içerisinde yer aldı. Sonuç olarak, MLVA Bank for Genotyping Microbes veri tabanında bulunan genotipler içerisinde Türkiye genotipleri birçok ülkeden bildirilen genotiplerle yakın genotipik yapıya sahip olduğu belirlendi.

Anahtar Kelimeler

• Antraks
• Bacillus anthracis
• MLVA-8
• moleküler epidemiyoloji

Molecular Epidemiology of Bacillus anthracis Genotypes Isolated From Turkey in Multilocus Variable Number of Tandem Repeat Analysis Databases

Öz

Anthrax is an important disease caused by Bacillus anthracis that threatens human and animal health worldwide. B. anthracis shows a genetically homogeneous structure because it can remain in the form of spores for a long time in nature. However, epidemiologically, it is possible to distinguish between strains with molecular methods such as multiple-locus variable-number tandem repeat analysis, single nucleotide repeat, and genome-wide single nucleotide polymorphism. In this study, it was aimed to analyze the B. anthracis MLVA-8 genotypes uploaded to the Bank for Genotyping Microbes database by multiple-locus variable-number tandem repeat analysis method. In this study, 2313 genotype profiles of 49 countries in the database were evaluated. In total, 402 genotypes were distributed within 24 clonal groups. Genotypes belonging to Turkey, on the other hand, show distribution in 8 groups. The highest number of genotypes (31%) among Turkey genotypes were localized in the XXIV clonal group. A total of 63.2% of the genotypes detected as a result of the analysis were included in the clonal groups IX, XXIII, and XXIV. As a result, among the genotypes in the MLVA Bank for Genotyping Microbes database, it was determined that the genotypes of Turkey were close to the genotypes reported from many countri

Anahtar Kelimeler

• Anthrax
• Bacillus anthracis
• MLVA-8
• molecular epidemiology

Kaynakça

1. 1. Koehler TM. Bacillus anthracis physiology and genetics. Mol Aspects Med. 2009;30(6):386-396. [CrossRef]
2. 2. Derzelle S, Thierry S. Genetic diversity of Bacillus anthracis in Europe: genotyping methods in forensic and epidemiologic investigations. Biosecur Bioterror. 2013;11(suppl 1):S166-S176. [CrossRef]
3. 3. Pilo P, Frey J. Pathogenicity, population genetics and dissemination of Bacillus anthracis. Infect Genet Evol. 2018;64:115-125. [CrossRef]
4. 4. WOAH. Anthrax (Terrestrial Manual, 8th edn, pp. 1–14). 2018.
5. 5. Zasada AA. Detection and identification of Bacillus anthracis: from conventional to molecular microbiology methods. Microorganisms. 2020;8(1) [CrossRef]
6. 6. Anisimova EA, FakhrutdinovNA, MirgazovDA, et al. Bacillus anthracis strain differentiation based on SNP and VNTR loci. Vavilovskii Zhurnal Genet Selektsii. 2022;26(6):560-567. [CrossRef]
7. 7. Wang D, Wang B, Zhu L, et al. Genotyping and population diversity of Bacillus anthracis in China based on MLVA and canSNP analysis. Microbiol Res. 2020;233:126414. [CrossRef]
8. 8. Rondinone V, Serrecchia L, Parisi A, et al. Genetic characterization of Bacillus anthracis strains circulating in Italy from 1972 to 2018. PLoS One. 2020;15(1):e0227875. [CrossRef]

9. 9. Keim P, Gruendike JM, Klevytska AM, Schupp JM, Challacombe J, Okinaka R. The genome and variation of Bacillus anthracis. Mol Aspects Med. 2009;30(6):397-405. [CrossRef]
10. 10. Keim P, Price LB, Klevytska AM, et al. Multiple-locus variable-number tandem repeat analysis reveals genetic relationships within Bacillus anthracis. J Bacteriol. 2000;182(10):2928-2936. [CrossRef]
11. 11. Grissa I, Bouchon P, Pourcel C, Vergnaud G. On-line resources for bacterial micro-evolution studies using MLVA or CRISPR typing. Biochimie. 2008;90(4):660-668. [CrossRef]
12. 12. Aslan S. Klinik örneklerden izole Edilen Brucella izolatlarının epidemiyolojik özelliklerinin multi-locus Variable Number Tandem Repeat Analysis ve Pulsed Field Gel Electrophoresis Yöntemleri ile Tespiti. Dissertation. Çukurova University; 2015.
13. 13. Letunic I, Bork P. Interactive Tree Of Life (iTOL) v5: an online tool for phylogenetic tree display and annotation. Nucleic Acids Res. 2021;49(W1):W293-W296. [CrossRef]
14. 14. Durmaz R, Doganay M, Sahin M, et al. Molecular epidemiology of the Bacillus anthracis isolates collected throughout Turkey from 1983 to 2011. Eur J Clin Microbiol Infect Dis. 2012;31(10):2783-2790. [CrossRef]
15. 15. Ortatatli M, Karagoz A, Percin D, Kenar L, Kilic S, Durmaz R. Antimicrobial susceptibility and molecular subtyping of 55 Turkish Bacillus anthracis strains using 25-loci multiple-locus VNTR analysis. Comp Immunol Microbiol Infect Dis. 2012;35(4):355-361. [CrossRef]
16. 16. Antwerpen M, Ilin D, Georgieva E, Meyer H, Savov E, Frangoulidis D. MLVA and SNP analysis identified a unique genetic cluster in Bulgarian Bacillus anthracis strains. Eur J Clin Microbiol Infect Dis. 2011;30(7):923-930. [CrossRef]
17. 17. Sue D, Marston CK, Hoffmaster AR, Wilkins PP. Genetic diversity in a Bacillus anthracis historical collection (1954 to 1988). J Clin Microbiol. 2007;45(6):1777-1782. [CrossRef]
18. 18. Van Ert MN, Easterday WR, Huynh LY, et al. Global genetic population structure of Bacillus anthracis. PLoS One. 2007;2(5):e461. [CrossRef]
19. 19. Pilo P, Perreten V, Frey J. Molecular epidemiology of Bacillus anthracis: determining the correct origin. Appl Environ Microbiol. 2008;74(9):2928-2931. [CrossRef]
20. 20. Fouet A, Smith KL, Keys C, et al. Diversity among French Bacillus anthracis isolates. J Clin Microbiol. 2002;40(12):4732-4734. [CrossRef]
21. 21. AikembayevAM, Lukhnova L, Temiraliyeva G, et al. Historical distribution and molecular diversity of Bacillus anthracis, Kazakhstan. Emerg Infect Dis. 2010;16(5):789-796. [CrossRef]
22. 22. Gierczyński R, Kałuzewski S, Rakin A, et al. Intriguing diversity of Bacillus anthracis in eastern Poland – the molecular echoes of the past outbreaks. FEMS Microbiol Lett. 2004;239(2):235-240. [CrossRef]
23. 23. Beyer W, Bellan S, Eberle G, et al. Distribution and molecular evolution of Bacillus anthracis genotypes in Namibia. PLOS Negl Trop Dis. 2012;6(3):e1534. [CrossRef]
24. 24. Fasanella A, Van Ert M, Altamura SA, et al. Molecular diversity of Bacillus anthracis in Italy. J Clin Microbiol. 2005;43(7):3398-3401. [CrossRef]
25. 25. Lista F, Faggioni G, Valjevac S, et al. Genotyping of Bacillus anthracis strains based on automated capillary 25-loci multiple locus variablenumber tandem repeats analysis. BMC Microbiol. 2006;6:33. [CrossRef]
26. 26. Wattiau P, Klee SR, Fretin D, et al. Occurrence and genetic diversity of Bacillus anthracis strains isolated in an active wool-cleaning factory. Appl Environ Microbiol. 2008;74(13):4005-4011. [CrossRef]
27. 27. Okutani A, Sekizuka T, Boldbaatar B, Yamada A, Kuroda M, Inoue S. Phylogenetic typing of Bacillus anthracis isolated in Japan by multiple locus variable-number tandem repeats and the comprehensive single nucleotide polymorphism. J Vet Med Sci. 2010;72(1):93-97. [CrossRef]
28. 28. Van Belkum A. Tracing isolates of bacterial species by multilocus variable number of tandem repeat analysis (MLVA). FEMS Immunol Med Microbiol. 2007;49(1):22-27. [CrossRef]
29. 29. Yudianingtyas DW, Sumiarto B, Susetya H, et al. Identification of the molecular characteristics of Bacillus anthracis (1982-2020) isolates in East Indonesia using multilocus variable-number tandem repeat analysis. Vet World. 2022;15(4):953-961. [CrossRef]
30. 30. Lienemann T, Beyer W, Pelkola K, et al. Genotyping and phylogenetic placement of Bacillus anthracis isolates from Finland, a country with rare anthrax cases. BMC Microbiol. 2018;18(1):102. [CrossRef]