Boğaların Testislerinde Bovine Viral Diyare Virüsünün Moleküler ve Patolojik Olarak Araştırılması

Öz

Bovine viral diarrhea virus (BVDV), gastrointestinal, solunum ve üreme sistemlerinde hastalıklara neden olan önemli bir patojendir. Ayrıca, üreme performansında azalmaya yol açar ve bu nedenle sığırların yönetiminde sürekli ekonomik kayıplara neden olur. Bu çalışmada, BVDV’nin boğa testisindeki varlığı indirekt immunofloresan (IF), immunohistokimya (IHC) ve ters transkriptaz polimeraz zincir reaksiyonu (RT-PCR) yöntemleriyle araştırıldı. Çalışmada, 100 boğa testis dokusu örneğinde BVD virusunun doku dağılımı patolojik ve virolojik olarak incelendi. Bu amaçla, IF, IHC ve RT-PCR yöntemleri kullanıldı. İndirekt IF yöntemiyle 21 (%21) testis örnek, IHC yöntemiyle 16 (%16) örnek pozitif bulundu. RT-PCR'da ise 13 (%13) örnek pozitif bulundu. Sunulan çalışmanın sonuçları, BVDV'nin boğa testisinde farklı hücre tiplerine enfekte olabileceğini ve virüsün öncelikle doğal veya suni tohumlama yoluyla bulaşabileceğini göstermektedir. Bu nedenle, boğalar hastalığın yayılmasında önemli bir epidemiyolojik faktördür.

Anahtar Kelimeler

• Bovine viral diyare virüsü
• immunfloresans
• immunohistokimya
• RT-PCR
• testis

Molecular and Pathological Survey of Bovine Viral Diarrhea Virus in the Testis of Bulls

Öz

Bovine viral diarrhea virus (BVDV) is an important pathogen that causes diseases in the gastrointestinal, respiratory, and reproductive systems. It also leads to a decrease in reproductive performance and consequently continuous economic losses in cattle management. The presence of BVD virus in bull testes was investigated in this study using indirect immunofluorescence (IF), immunohistochemistry (IHC), and Reverse Transcription polymerase chain reaction (RT-PCR) methods. The tissue distribution of BVDV was examined pathologically and virologically in 100 bull testis tissue samples. For this purpose, IF, IHC, and RT-PCR methods were employed. Positive IF staining was detected in 21 (21%) testis samples using the indirect IF method. In IHC staining, 16 (16%) samples were found to be positive. In RT-PCR, 13 (13%) samples tested positive. The results of the presented study demonstrate that BVDV can infect different cell types in bull testes and that the virus can primarily be transmitted through natural or artificial insemination. Therefore, bulls are an important epidemiological factor in the transmission of the disease.

Anahtar Kelimeler

• Bovine viral diarrhea virus
• immunoflourescence
• immunohistochemistry
• RT-PCR
• testis

Kaynakça

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