The Intensity of Lipid Peroxide Oxidation Processes and the System State of Antioxidant Protection of Broiler Chicken Due to the Action of the Synbiotic Preparation in Complex with the Disinfectant

Öz

Developing methods for increasing the immune reactivity and antioxidant potential of the bird's body during critical periods of growth is an urgent task today. The purpose of the research was to find out the influence of the synbiotic "Biomagn" in combination with the disinfectant "Diolide" on the intensity of the processes of peroxide oxidation of lipids and the activity of the system of antioxidant protection in the organism of chickens. The research was carried out on 2 groups of chickens, 100 in each, starting from 1 to 41 days of age: the control group was fed with standard compound feed (SCF); the chickens of the experimental group were fed with SCF, and the synbiotic preparation "Biomagn" based on 0.5 kg per ton of compound feed. The experimental group received a solution of the preparation "Diolide" with water. For conducting immunological research, blood was taken from chickens at different age periods: from 10-, 27-, 31-, and 41-day-old chickens. The use of the synbiotic preparation "Biomagn" in combination with the disinfectant "Diolide" in the chickens of the experimental group normalized the intensity of the processes of lipid peroxidation and oxidative modification of proteins in the poultry's organism - a decrease was established (p <0.05–0.001) in GPO content and TBK-active products and aldehyde derivatives oxidative modification of proteins in the blood compared to the control. The detected event was determined by increased activity of the enzyme link of the antioxidant protection system of the organism of chickens.

Anahtar Kelimeler

• Antioxidant protection
• chickens
• chlorine dioxide
• lipid peroxide oxidation

Lipid Peroksit Oksidasyon Proseslerinin Yoğunluğu ve Dezenfektanla Kompleks İçinde Sinbiyotik Preparatın Etkisine Bağlı Broyler Tavuğun Antioksidan Korumasının Sistem Durumu

Öz

Büyüme sürecinin kritik dönemlerinde kanatlılarda vücudun bağışıklık tepkisini ve antioksidan potansiyelini artırmaya yönelik yöntemler geliştirmek günümüzde acil bir görevdir. Araştırmanın amacı, "Biomagn" sinbiyotiğinin "Diolide" dezenfektanı ile birlikte tavukların organizmasındaki lipid peroksit oksidasyon süreçlerinin yoğunluğu ve antioksidan koruma sisteminin aktivitesi üzerindeki etkisini belirlemektir. Araştırma, 1 günlük yaştan 41 günlük yaşa kadar yetiştirilen ve her birinde 100 tavuk bulunan 2 grup üzerinde gerçekleştirilmiştir: kontrol grubu standart karma yem (SKY) ile beslenmiştir; deney grubundaki tavuklar ise SKY ve 0.5 kg/ton oranında "Biomagn" sinbiyotik preparatı ile beslenmiştir. Deney grubuna "Diolide" preparatının su ile çözeltisi verilmiştir. İmmünolojik araştırmalar için tavuklardan farklı yaş dönemlerinde (10-, 27-, 31- ve 41 günlük tavuklardan) kan alınmıştır. Sinbiyotik preparat "Biomagn" ile dezenfektan "Diolide"nin birlikte kullanımı, deney grubundaki tavukların organizmasındaki lipid peroksidasyon süreçlerinin yoğunluğunu ve proteinlerin oksidatif modifikasyonunu normalleştirmiştir - kanlarındaki GPO içeriği ve TBK-aktif ürünler ile aldehit türevleri oksidatif modifikasyonu kontrol grubuna göre azalmıştır (p <0.05–0.001). Tespit edilen etki, tavukların organizmasının antioksidan koruma sisteminin enzim bağlantısının aktivitesindeki artışla belirlenmiştir.

Anahtar Kelimeler

• antioksidan koruma
• klor dioksit
• lipit peroksit oksidasyonu
• probiyotik
• tavuk

Kaynakça

1. 1. Shevchuk M, Stoyanovskyy V, Kolomiiets I. Technological stress in poultry. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 2018, 20(88), 63-68.
2. 2. Osadcha, Yu., Shuliar, A., Sydorenko, O., Dzhus, P., & Shuliar, A. (2023). Biochemical parameters of chicken blood under the influence of technological stimuli of various etiologies. Scientific Horizons, 26(9), 70-80.
3. 3. Lavryshyn, Y., Varkholyak, I., Martyschuk, T., Guta, Z., & Ivankiv, L. (2016). THE BIOLOGICAL SIGNIFICANCE OF THE ANTIOXIDANT DEFENSE SYSTEM OF ANIMALS BODY. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 18(2(66), 100-111.
4. 4. Antonyak GL, Babych NO, Sologub LI, Snitynsky VV. Formation of reactive oxygen species and the system of antioxidant protection in the animal organism. Anim Biol. 2000; 2(2): 34-42.
5. 5.Kalyuzhin VA. Thermoresistance in a yeast Saccharomyces cerevisiae. Zh Obshch Biol. 2011; 72(2): 140-149.
6. 6.Regoli F, Gorbi S, Frenzilli G, et al. Oxidative stress in ecotoxicology: from the analysis of individual antioxidants to a more integrated approach. Mar Environ Res. 2002; 54(3-5):419-423.
7. 7.Lushchak VI, Bagnyukova TV, Luzhna LI. Indicators of oxidative stress.2. peroxides of lipids. Ukr biochim Zh. 2006;78(6):113-120.
8. 8. MylostуvaD. F., Farafonov, S. J., PuzniakO. М., StakhivV. І., Borshchenko, V. V., Tsisinska, S. V., & Voloshin, S. V. (2023). Antioxidant system of the body of young Ukrainian beef cattle under the action of microelements . Regulatory Mechanisms in Biosystems, 14(1), 106-111.

9. 9. Romanovych, L., Kurtyak, B., & Mudrak, D. (2019). The intensity of the processes of oxidative modification of proteins and the activity of enzymes of the antioxidant protection system of broiler chickens under the action of vitamins E and C. Ukrainian Journal of Veterinary and Agricultural Sciences, 2(3), 19-22.
10. 10. Klaenhammer TR, Kleerebezem M, Kopp MV, Rescigno M. The impact of probiotics and prebiotics on the immune system. Nat Rev Immunol. 2012;12(10):728-734.
11. 11. Stoyanovskyy, V. G., Shevchuk, M. O., Kolomiiets, I. A., & Kolotnytskyy, V. A. (2020). Dynamics of individual indicators of protein metabolism in the body of broiler chickens on the background of combined stress when included in the diet “Reasil Humic Vet” + “Laktin” and “Reasil Humic Health”. Ukrainian Journal of Veterinary and Agricultural Sciences, 3(2), 42-46.
12. 12. Mishra V, Shah C, Mokashe N, Chavan R, Yadav H, Prajapati J. Probiotics as Potential Antioxidants: A systematic review. J Agric Food Chem. 2015; 63(14):3615-3626.
13. 13. Irshad A. Effect of probiotics on broilers performance. Int J Poultry Sci. 2006;5(6):593-597.
14. 14. Sander JE, Hofacre CL, Cheng IH, Wyatt RD. Investigation of resistance of bacteria from commercial poultry sources to commercial disinfectants. Avian Dis. 2002;46(4):997-1000.
15. 15. Wu BQ, Zhang T, Guo LQ, Lin JF. Effects of Bacillus subtilis KD1 on broiler intestinal flora. Poult Sci. 2011;90(11): 2493-2499.
16. 16. Horiuk Y, Kukhtyn M, Kovalenko V, Kornienko L, Horiuk V, Liniichuk N. Biofilm formation in bovine mastitis pathogens and the effect on them of antimicrobial drugs. Independent J Man Prod. 2019;10(7):897-910.
17. 17. Chechet O, Kovalenko V, Haidei O, Polupan I, Rudoi O. Toxicity and virucidal activity of chlorine dioxide disinfectant. Sci Horiz. 2022;25(5):30-39.
18. 18. Du Y, Lv XT, Wu QY, et al. Formation and control of disinfection byproducts and toxicity during reclaimed water chlorination: A review. J Environ Sci (China). 2017; 58:51-63.
19. 19. Ma JW, Huang BS, Hsu CW, et al. Efficacy and safety evaluation of a chlorine dioxide solution. Int J Environ Res Public Health. 2017; 14(3):329.
20. 20. Park HW, Chen G, Hwang CA, Huang L. Effect of water activity on inactivation of Listeria monocytogenes using gaseous chlorine dioxide - A kinetic analysis. Food Microbiol. 2021;95:103707.
21. 21. Field and laboratory methods for grassland and animal production research. L. 't Mannetje (Editor), R.M. Jones. Research output: 452, 2000. Academic, 10.1079/9780851993515.0000
22. 22. Buckmaster C. Shifting the culture of lab animal care. Lab Anim (NY). 2012;41(7):205.
23. 23. DIRECTIVE 2010/63/EU OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL of 22 September 2010 on the protection of animals used for scientific purposes. https://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2010:276:0033:0079:en:PDF
24. 24. European Convention for the Protection of Vertebrate Animals used for Experimental and Other Scientific Purposes. https://rm.coe.int/168007a67b
25. 25. Studenok, A., Shnurenko, E., Karpovskyi, V., Trokoz, V., & Gutyj, B. (2021). Indicators of protein metabolism and intensity of lipid peroxide oxidation in chickens with different vegetative status. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 23(102), 110-118.
26. 26. Chechet OM, Kovalenko VL, Vishchur OI, Haidei OS, Liniichuk NV, Krushelnytska OV. The activity of T- and B-cell links of specific protection of chicken-broilers under the influence of synbiotic preparation “Biomagn” and “Diolide” disinfectant. Ukrainian J Vet Agric Sci. 2022; 5(1): 46-52.
27. 27. Chechet OM, Kovalenko VL, Gaidei OS. Preclinical tests of the preparation "Biomagn" on laboratory animals and with the use of Tetrahymena pyriformis. Med Clin Chem. 2021;23(3):48-56.
28. 28. Jiang S, Yan FF, Hu JY, Mohammed A, Cheng HW. Bacillus subtilis-based probiotic improves skeletal health and immunity in broiler chickens exposed to heat stress. Animals. 2021;11(6):1494.
29. 29. Aluwong T, Kawu M, Raji M, et al. Effect of yeast probiotic on growth, antioxidant enzyme activities and malondialdehyde concentration of broiler chickens. Antioxidants. 2013;2(4):326-339.
30. 30. Wang J, Ishfaq M, Miao Y, et al. Dietary administration of Bacillus subtilis KC1 improves growth performance, immune response, heat stress tolerance, and disease resistance of broiler chickens. Poultry Sci. 2022;101(3):101693.
31. 31. Ahmed-Farid O, Salah AS, Nassan MA, El-Tarabany MS. Effects of chronic thermal stress on performance, energy metabolism, antioxidant activity, brain serotonin, and blood biochemical indices of broiler chickens. Animals. 2021;11(9):2554.
32. 32. Belenichev IF, Kovalenko SI, Dunaev VV. Antioxidants: modern idea, prospects of creation. Medicines. 2002;1: 25-29.
33. 33. Simonov MR, Kulyk MM. Changes in the activity of antioxidant system of chickens ISA BROWN CROSS depending of age. Biol Tvarin. 2019;9(1-2):56-61.
34. 34. Gudyma V Yu, Yanovych VG. The content of lipid peroxidation products and the activity of antioxidant enzymes in the tissues of laying hens at different levels of vitamin D3 in the ration. Sci Bull Stepan Gzhytsky LNUVMBT. 2010;2(44): 55-58.
35. 35. Romanovich NN, Kurtyak BM, Romanovich MS, Vishchur OI, Mudrak DI, Matiukha IO. Dynamics of intensity of the protein oxidative modification processes and the state of the antioxidant protection system in chicken-broilers under the effect of bps-44 and the dried preparations of Saccharomyces cerevisiae. Biol Tvarin. 2019;21(1):48-54.
36. 36. Kim K, He Y, Xiong X, et al. Dietary supplementation of Bacillus subtilis influenced intestinal health of weaned pigs experimentally infected with a pathogenic E. coli. J Anim Sci Biotech. 2019;10(1):1-12.
37. 37. Schieber M, Chandel NS. ROS function in redox signaling and oxidative stress. Curr Biol. 2014;24(10): R453-R462.
38. 38. Zhao Y, Zeng D, Wang H, et al. Dietary probiotic Bacillus licheniformis H2 enhanced growth performance, morphology of small intestine and liver, and antioxidant capacity of broiler chickens against Clostridium perfringens-induced subclinical necrotic enteritis. Probiotics Antimicrob Proteins. 2020;12(3):883-895.
39. 39. Gong L, Wang B, Mei X, et al. Effects of three probiotic Bacillus on growth performance, digestive enzyme activities, antioxidative capacity, serum immunity, and biochemical parameters in broilers. Anim Sci J. 2018;89(11): 1561-1571.
40. 40. Yu Y, Li Q, Zeng X, et al. (2022). Effects of probiotics on the growth performance, antioxidant functions, immune responses, and caecal microbiota of broilers challenged by lipopolysaccharide. Front Vet Sci. 2022;9:846649.
41. 41. Xu Y, Yu Y, Shen Y, et al. Effects of Bacillus subtilis and Bacillus licheniformis on growth performance, immunity, short chain fatty acid production, antioxidant capacity, and cecal microflora in broilers. Poultry Sci. 2021;100(9):101358.
42. 42. Zhao Y, Zeng D, Wang H, et al. Dietary probiotic Bacillus licheniformis H2 enhanced growth performance, morphology of small intestine and liver, and antioxidant capacity of broiler chickens against Clostridium perfringens-induced subclinical necrotic enteritis. Probiotics Antimicrob Proteins. 2020;12(3): 883-895.
43. 43. Ji L, Zhang L, Liu H, et al. Bacillus subtilis M6 improves intestinal barrier, antioxidant capacity and gut microbial composition in AA broiler. Front Nutr. 2022;17(9):965310.