Investigation of Oxidative Stress Parameters in Cattle Infected with Mycobacterium avium subsp. Paratuberculosis

Öz

Paratuberculosis is a zoonotic disease caused by Mycobacterium avium subsp. paratuberculosis (MAP) in cattle. MAP may cause the formation of reactive oxygen species (ROS) by increasing the release of proinflammatory cytokines in the host. Due to the increase in ROS, the oxidant-antioxidant balance may be disrupted and oxidative stress may occur. The aim of the study was to determine the oxidative stress parameters in cattle infected with paratuberculosis. For this purpose, 15 cattle sera that were positive for paratuberculosis and 15 clinically healthy 30 cattle sera were used as the control group. In the samples taken, oxidative stress parameters such as total antioxidant capacity (TAS), total oxidant capacity (TOS), oxidative stress index (OSI), native thiol, total thiol and disulphide levels were evaluated. According to our study results, TOS (21.911±11.80), OSI (37.99±21.40), total thiol (1836.671±877.06) and disulphide (715.420±395.32) values in the paratuberculosis group were significantly higher than TOS (8.538±5.18), OSI (10.24±7.23), total thiol (823.809±289.86) and disulphide (197.936±131.70) values in the control group (P<.001). The TAS (0.588±0.14) value in the paratuberculosis group was significantly lower than the TAS (0.952±0.26) value in the control group (P<.001). No significant difference was found between the two groups in terms of native thiol levels (P>.05). As a result, it was determined that the oxidant-antioxidant balance was disrupted and oxidative stress occurred in MAP infected cattle. Therefore, it was concluded that oxidative stress parameters can be used as biomarkers in the diagnosis and treatment of the disease.

Anahtar Kelimeler

• oxidative stress
• cattle
• Paratuberculosis

Mycobacterium avium subsp. paratuberculosis ile Enfekte Sığırlarda Oksidatif Stres Parametrelerinin İncelenmesi

Öz

Paratüberküloz sığırlarda Mycobacterium avium subsp. paratuberculosis (MAP) tarafından oluşturulan zoonotik bir hastalıktır. MAP konakçıda proinflamatuar sitokinlerin salımını artırarak reaktif oksijen türleri (ROS) oluşumuna neden olabilir. ROS artışına bağlı olarak da oksidan antioksidan denge bozulabilir ve oksidatif stres ortaya çıkabilir. Çalışmanın amacı paratüberküloz enfekte sığırlarda oksidatif stres parametrelerinin belirlenmesidir. Bu amaçla çalışmada paratüberküloz yönünden pozitif tespit edilmiş 15 adet sığır serumu ve kontrol grubu olarak 15 adet klinik açıdan sağlıklı toplam 30 adet sığır serumu kullanılmıştır. Alınan numunelerde oksidatif stres parametrelerinden total antioksidan kapasite (TAK), total oksidan kapasite (TOK), oksidatif stres indeksi (OSİ), native thiol, total thiol ve disülfid düzeyleri değerlendirilmiştir. Çalışma sonuçlarımıza göre paratüberküloz grupta TOK (21,911±11,80), OSİ (37,99±21,40), total thiol (1836,671±877,06) ve disülfid (715,420±395,32) değerleri kontrol grubundaki TOK (8,538±5,18), OSİ (10,24±7,23), total thiol (823,809±289,86) ve disülfid (197,936±131,70) değerlerine göre anlamlı düzeyde yüksek belirlenmiştir (P<,001). Paratüberküloz grubunda ki TAK (0,588±0,14) değeri ise kontrol grubunda ki TAK (0,952±0,26) değerine göre anlamlı düzeyde düşük belirlenmiştir (P<,001). Native thiol düzeyleri açısından her iki grup arasında anlamlı bir fark belirlenememiştir (P>,05). Sonuç olarak MAP enfekte sığırlarda oksidan antioksidan dengenin bozulduğu ve oksidatif stresin ortaya çıktığı belirlenmiştir. Bu nedenle hastalığın tanı ve tedavisinde oksidatif stres parametrelerinin biyobelirteç olarak kullanılabileceği kanaatine varılmıştır.

Anahtar Kelimeler

• Oksidatif stres
• paratüberküloz
• sığır

Kaynakça

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