Sıçanlarda Azitromisin ile Oluşturulan Kalp Hasarına Karşı Krisin’in Etkilerinin Araştırılması

Öz

Azitromisin (AZM), üst ve alt solunum yolu enfeksiyonlarının tedavisinde kullanılan bir makrolid antibiyotiktir. Terapötik etkilerinin yanında kardiyak ve oksidatif hasar gibi olumsuz etkilere sahiptir. Propoliste ve çeşitli bitkilerde bulunan Krisin (CHR), antioksidan özelliğiyle bilinen doğal bir flavonoiddir. Bu çalışmada, geniş spektrumlu bir antibiyotik olan AZM’nin neden olduğu kalp hasarına karşı CHR’nin koruyucu etkisi araştırıldı. Bu amaçla, yirmi sekiz dişi sıçan Kontrol, CHR, AZM, AZM+CHR olmak üzere dört gruba ayrıldı. AZM (200 mg/kg) ve CHR (50 mg/kg) yedi gün boyunca günde bir kez oral yoldan uygulandı. Kalp dokusunda hasarı belirlemek için kardiyak belirteçler ve oksidatif stres parametreleri analiz edildi. Doku hasarını ve yapısal değişiklikleri tespit etmek için histopatolojik analizler yapıldı. Bu analizler sonucunda elde edilen verilere göre AZM, kalp dokusunda laktat dehidrogenaz (LDH), kreatin kinaz-miyokardiyal bant (CK-MB) aktiviteleri ve kardiyak troponin-I (cTn-I) seviyesini artırdı. AZM toksikasyonu, süperoksit dismutaz (SOD), glutatyon peroksidaz (GPx), katalaz (CAT) aktiviteleri ve glutatyon (GSH) seviyeleri gibi antioksidan enzimlerin aktivitelerini azaltırken, malondialdehit (MDA) seviyelerini önemli ölçüde artırmıştır. AZM+CHR tedavisinin kalp dokusu kardiyak belirteçlerinde (LDH, CK-MB, cTn-I) azalma gösterdiği tespit edildi. Ayrıca, CHR tedavisinin AZM ile birlikte uygulanması MDA düzeyini düşürmüş ve GSH düzeyini ve GPx, SOD ve CAT aktivitelerini artırmıştır. Elde edilen bulgular birlikte değerlendirildiğinde, AZM’nin kardiyak belirteçleri ve oksidatif stresi artırarak kalp hasarına neden olduğu, CHR destekleyici tedavisinin ise bu parametreleri normale yakınlaştırarak hasarı azalttığı tespit edildi.

Anahtar Kelimeler

• Azitromisin
• Chrysin
• Kalp
• Oksidatif stres
• Rat

Investigation of the Effects of Chrysin Against Azithromycin-Induced Heart Damage in Rats

Abstract

Azithromycin (AZM) is macrolide antibiotic used to treat infections of the upper and lower respiratory tract. In addition to its therapeutic effects, it has adverse effects such as cardiac and oxidative damage. Chrysin (CHR), which is found in propolis and various plants, is a natural flavonoid known for its antioxidant properties. In this study, we investigated the protective effect of CHR against cardiac damage caused by AZM, a broad-spectrum antibiotic. For this purpose, twenty-eight female rats were divided into four groups: Control, CHR, AZM, AZM+CHR. AZM (200 mg/kg) and CHR (50 mg/kg) were administered orally once daily for seven days. Cardiac markers and oxidative stress parameters were analyzed to determine heart tissue damage. Histopathological analyses were performed to detect tissue damage and structural changes. According to the data obtained from these analyses, AZM increased lactate dehydrogenase (LDH) and creatine kinase-myocardial band (CK-MB) activities and cardiac troponin-I (cTn-I) levels in the heart tissue. AZM toxication significantly increased malondialdehyde (MDA) levels while reducing the activities of antioxidant enzymes such as superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) activities and glutathione (GSH) levels. AZM+CHR treatment decreased cardiac tissue cardiac markers (LDH, CK-MB, and cTn-I). In addition, CHR treatment together with AZM decreased MDA levels and increased GSH levels and GPx, SOD, and CAT activities. When the findings were evaluated together, it was determined that AZM caused heart damage by increasing cardiac markers and oxidative stress, while CHR supplementation reduced the damage by bringing these parameters closer to normal.

Keywords

• Azithromycin
• Chrysin
• Heart
• Oxidative stress
• Rat

Kaynakça

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