Arı Ekmeği (Perga) ve Polenin Bıldırcınların Karaciğer Dokusunda Katalaz Salınımı ve Serum Antioksidan Düzeyi Üzerine Etkisi

Yıl 2025, Cilt: 20 Sayı: 3, 110 - 116, 25.12.2025

Ebru Karadağ Sarı , Mükremin Ölmez , Gizem Fidan Arslan , Şükran Aras , Mustafa Makav , Tarkan Şahin

https://doi.org/10.17094/vetsci.1696736

https://izlik.org/JA84ZS92TC

Öz

Bu çalışma, arı ekmeği (perga, BB) ve polenin bıldırcın karaciğer dokusunda katalaz immünoreaktivitesi ve serum antioksidan düzeyleri üzerindeki etkilerini belirlemek amacıyla gerçekleştirilmiştir. Çalışmada, 30 adet 1 günlük erkek Japon bıldırcını (Coturnix coturnix japonica) kullanılmıştır. Bıldırcınlar kontrol, arı ekmeği ve polen grupları olmak üzere üç gruba ayrılmıştır. Hayvanlar çalışmanın 1., 14. ve 28. günlerinde tartılmış ve tüm gruplar için canlı ağırlık değerleri belirlenmiştir. Vücut ağırlığı artışı, tartımlardan elde edilen farklardan hesaplandı. Çalışmanın sonunda, çalışmanın sonunda alınan kan örneklerinden ekstrakte edilen serumlarda malondialdehit (MDA) ve glutatyon (GSH) değerleri belirlendi ve karaciğer dokuları alındı ve rutin histolojik ve immünohistokimyasal prosedürler uygulandı. Bıldırcın karaciğer dokularında katalaz immünoreaktivitesi değerlendirildi. MDA düzeyleri açısından polen grubu ile kontrol grubu arasında istatistiksel olarak anlamlı bir fark vardı. Tüm grupların karaciğer dokusunda güçlü katalaz immünoreaktivitesi tespit edildi. Sonuç olarak, diyete polen ilavesinin MDA düzeylerinin azalmasına olumlu katkı sağlayacağı düşünülmektedir.

Anahtar Kelimeler

arı ekmeği , bıldırcın , karaciğer , polen

The Effects of Bee Bread (Perga) and Pollen on Liver Catalase Activity and Serum Antioxidant Levels in Quails

https://izlik.org/JA84ZS92TC

Öz

This study was carried out to determine the effects of bee bread (perga, BB) and pollen on catalase immunoreactivity and serum antioxidant levels in liver tissue of quails. In the study, 30 1-day-old male Japanese quails (Coturnix coturnix japonica) were used. The quails were divided into three groups as control, bee bread and pollen groups. The animals were weighed on days 1, 14, and 28 of the study, and live weight values were determined for all groups. Body weight gain was calculated from the differences obtained from the weighings. At the end of the study, malondialdehyde (MDA) and glutathione (GSH) values were determined in the sera extracted from the blood samples taken at the end of the study, and liver tissues were taken and routine histological and immunohistochemical procedures were performed. Catalase immunoreactivity was evaluated in quail liver tissues. There was a statistically significant difference between the pollen group and the control group in terms of MDA levels. Strong catalase immunoreactivity was detected in the liver tissue of all groups. As a result, it is thought that the addition of pollen to the diet will contribute positively to the reduction of MDA levels.

Anahtar Kelimeler

Bee bread , liver , pollen , quails

Etik Beyan

Approval was received for this research from Kafkas University Experimental Animals Ethics Committee (dated 25.10.2021, numbered 2021/10 and approval number KAU-HADYEK/2021-160)

Kaynakça

• 1. Barajas J, Cortes-Rodriguez M, Rodríguez-Sandoval E. Effect of temperature on the drying process of bee pollen from two zones of Colombia. J Food Process Eng. 2012;35(1):134-148.
• 2. Markiewicz-Żukowska R, Naliwajko SK, Bartosiuk E, et al. Chemical composition and antioxidant activity of beebread, and its influence on the glioblastoma cell line (U87MG). J Apic Sci. 2013;57(2):147-157.
• 3. Fuenmayor B, Zuluaga D, Díaz M, et al. Evaluation of the physicochemical and functional properties of Colombian bee pollen. Rev MVZ Córdoba. 2014;19(1):4003-4014.
• 4. Zuluaga CM, Juan C, Serrato M, Quicazan, C. Chemical, nutritional and bioactive Characterization of Colombian bee-bread. Chem Eng Trans. 2015;43:175-180.
• 5. Kieliszek M, Piwowarek K, Kot AM, Blažejak S, Chlebowska-Śmigiel A, Wolska I. Pollen and bee bread as new health-oriented products: A review. Trends Food Sci Technol. 2018;71:170-180.
• 6. Kumar R, Kashyap L. Bee pollen as healthy food. J Ent Res. 2023;47(2):454-457.
• 7. Nemauluma MFD, Manyelo TG, Ng'ambi JW, Kolobe DS, Malematja E. Effects of bee pollen inclusion on performance and carcass characteristics of broiler chickens. Poult Sci. 2023;102(6):102628.
• 8. Boulfous N, Belattar H, Ambra R, Pastore G, Ghorab A. Botanical origin, phytochemical profile, and antioxidant activity of bee pollen from the mila region, algeria. Antioxidants. 2025;14(3):291.
• 9. Kebede AI, Gebremeskel FH, Ahmed AD, Dule G. Bee products and their processing: a review. Pharm Pharmacol Int J. 2024;12(1):5-12.
• 10. Ölmez M, Şahin T, Karadağoğlu Ö, et al. Effect of herbal extract mixture on growth performance and antioxidant parameters in broilers. J Hellenic Vet Med Soc. 2022;73(2): 4069–4076.
• 11. Chandimali N, Bak SG, Park EH, et al. Free radicals and their impact on health and antioxidant defenses: a review. Cell Death Dis. 2025;11:19.
• 12. Duan J, Dong W, Wang G, et al. Senescence-associated 13-HODE production promotes age-related liver steatosis by directly inhibiting catalase activity. Nat Commun. 2023;14:8151.
• 13. Chia Tan Y, Abdul Sattar M, Ahmeda AF, et al. Apocynin and catalase prevent hypertension and kidney injury in Cyclosporine A-induced nephrotoxicity in rats. Plos One. 2020;15(4): e0231472.
• 14. Junqueira LC, Carneiro J: Basic Histology. 10th ed., Çeviri Editörleri: Aytekin Y, Solakoğlu S. Temel Histoloji. Nobel Tıp Kitabevleri, İstanbul. 2006;332-344.
• 15. Mayda N. Arı polleni ve arı ekmeğinin palinolojik kimyasal ve antioksidan kapasitelerinin belirlenmesi, Hacettepe Üniversitesi, Yüksek Lisans Tezi, 2019.
• 16. NRC. Nutrient requirements of poultry, 9th revised edn. National Academy Press, Washington, DC, USA. 1994.
• 17. Yediel Aras Ş, Makav M, Kuru M, et al. The effect of quercetin application on desmin and vimentin levels in ovariectomized rats with cyclophosphamide-ınduced cardiotoxicity. Kafkas Univ Vet Fak Derg. 2025;31(3):377-385.
• 18. Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. J Lab Clin Med. 1963;61:882-888.
• 19. Yoshioka T, Kawada K, Shimada T, Mori M. Lipid peroxidation in maternal and cord blood and protective mechanism against activated-oxygen toxicity in the blood. Am J Obstet Gynecol. 1979;135:372-376.
• 20. Hada LV, Johri A. The study of pollen biology, palynology and pollen production to estimate pollen-ovule ratio in datura innoxia mill. IJRTI. 2023;8(2):342-350.
• 21. Bogdanov S. Pollen: Nutrition, Functional Properties, Health: A Review. Bee Product Science. 2011;1-34.
• 22. Cordiano R, Di Gioacchino M, Mangifesta R, Panzera C, Gangemi S, Minciullo PL. Malondialdehyde as a Potential Oxidative Stress Marker for Allergy-Oriented Diseases: An Update. Molecules. 2023;28(16):5979.
• 23. Saez GT, Bannister WH, Bannister JV. Free radicals and thiol compounds-The role of glutathione against free radical toxicity. In: Vina J (ed). Glutathione Metabo Physiological Functions. Boca Raton, FL: CRC Press. Inc. 1990;237-254.
• 24. Coles B, Ketterer B. The role of glutathione transferases. Crit Rev Biochem Mol Biol. 1990;25:47-70.
• 25. Majrashi KA. Effects of supplementing quails’ (coturnix japonica) diets with a blend of clove (syzygium aromaticum) and black cumin (nigella sativa) oils on growth performance and health aspects. Life. 2022;12(11):1915.
• 26. Babaei S, Rahimi S, Torshizi MAK, Tahmasebi G, Miran SNK. Effects of propolis, royal jelly, honey and bee pollen on growth performance and immune system of Japanese quails. Vet Res Forum. 2016;7(1):13-20.
• 27. Tatlı Seven P, Sur Arslan A, Seven İ, Gökçe Z. The effects of dietary bee pollen on lipid peroxidation and fatty acids composition of Japanese quails (Coturnix coturnix japonica) meat under different stocking densities. J Appl Anim Res. 2016;44(1):487-491.
• 28. Canogullari S, Baylan M, Sahinler N, Sahin A. Effects of propolis and pollen supplementations on growth performance and body components of Japanese quails (Coturnix coturnix japonica). Arch Geflugelk. 2009;73(3):173-178.
• 29. Farag SA, El-Rayes TK. Research article effect of beepollen supplementation on performance, carcass traits and blood parameters of broiler chickens. Asian J Anim Vet Adv. 2016;11(3):168-177.
• 30. Çetintaş B. Diyete soy isoflavon ilavesinin bıldırcınlarda (coturnix coturnix japonica) karaciğer ve kas dokularındaki delta 9, 6, 5 desatüraz ürünleri ve kolesterol üzerine etkileri. Fırat Universitesi, Fen Bilimleri Enstitüsü, Yüksek Lisans Tezi, Elazığ. 2006.
• 31. Su T, Wang P, Li H, et al. The Arabidopsis catalase triple mutant reveals ımportant roles of catalases and peroxisome-derived signaling in plant development. J. Integr. Plant Biol. 2018;60:591–607.
• 32. Karaali HF, Fahmi RR, Borjac JM. Effect of Ocimum basilicum leaves extract on acetaminophen-induced nephrotoxicity in BALB/c mice. J Complement Integr Med. 2018;16(2):1-14.
• 33. Joshi D, Srivastav SK, Belemkar S, Dixit VA. Zingiber officinale and 6-gingerol alleviate liver and kidney dysfunctions and oxidative stress induced by mercuric chloride in male rats: A protective approach. Biomed Pharmacother. 2017;91:645-655.
• 34. Zoidis E, Pappas AC, Georgiou CA, Komaitis E, Feggeros K. Selenium affects the expression of GPx4 and catalase in the liver of chicken. Comp Biochem Physiol B Biochem Mol Biol. 2010;155(3):294-300.
• 35. Grzegorzewska AK, Wolak D, Hrabia A. Effect of tamoxifen treatment on catalase (CAT) and superoxide dismutase (SOD) expression and localization in the hen oviduct. Theriogenology. 2024;214:73-80.
• 36. Zhu W, Li YH, Chen ML, Hu FL. Protective effects of Chinese and Brazilian propolis treatment against hepatorenal lesion in diabetic rats. Hum Exp Toxicol. 2011;30:1246-1255.
• 37. Karan M, Baygeldi SB, Özkan ZE, et al. Japon bıldırcınlarında (coturnix coturnix japonica) karaciğerin morfolojik yapısının incelenmesi. FÜ Sağ Bil Vet Derg. 2018;32(3):209-212.