Arı Ekmeği (Perga) ve Polenin Bıldırcınların Karaciğer Dokusunda Katalaz Salınımı ve Serum Antioksidan Düzeyi Üzerine Etkisi

Öz

Bu çalışma, arı ekmeği (perga, BB) ve polenin bıldırcın karaciğer dokusunda katalaz immünoreaktivitesi ve serum antioksidan düzeyleri üzerindeki etkilerini belirlemek amacıyla gerçekleştirilmiştir. Çalışmada, 30 adet 1 günlük erkek Japon bıldırcını (Coturnix coturnix japonica) kullanılmıştır. Bıldırcınlar kontrol, arı ekmeği ve polen grupları olmak üzere üç gruba ayrılmıştır. Hayvanlar çalışmanın 1., 14. ve 28. günlerinde tartılmış ve tüm gruplar için canlı ağırlık değerleri belirlenmiştir. Vücut ağırlığı artışı, tartımlardan elde edilen farklardan hesaplandı. Çalışmanın sonunda, çalışmanın sonunda alınan kan örneklerinden ekstrakte edilen serumlarda malondialdehit (MDA) ve glutatyon (GSH) değerleri belirlendi ve karaciğer dokuları alındı ve rutin histolojik ve immünohistokimyasal prosedürler uygulandı. Bıldırcın karaciğer dokularında katalaz immünoreaktivitesi değerlendirildi. MDA düzeyleri açısından polen grubu ile kontrol grubu arasında istatistiksel olarak anlamlı bir fark vardı. Tüm grupların karaciğer dokusunda güçlü katalaz immünoreaktivitesi tespit edildi. Sonuç olarak, diyete polen ilavesinin MDA düzeylerinin azalmasına olumlu katkı sağlayacağı düşünülmektedir.

Anahtar Kelimeler

• arı ekmeği
• bıldırcın
• karaciğer
• polen

The Effects of Bee Bread (Perga) and Pollen on Liver Catalase Activity and Serum Antioxidant Levels in Quails

Abstract

This study was carried out to determine the effects of bee bread (perga, BB) and pollen on catalase immunoreactivity and serum antioxidant levels in liver tissue of quails. In the study, 30 1-day-old male Japanese quails (Coturnix coturnix japonica) were used. The quails were divided into three groups as control, bee bread and pollen groups. The animals were weighed on days 1, 14, and 28 of the study, and live weight values were determined for all groups. Body weight gain was calculated from the differences obtained from the weighings. At the end of the study, malondialdehyde (MDA) and glutathione (GSH) values were determined in the sera extracted from the blood samples taken at the end of the study, and liver tissues were taken and routine histological and immunohistochemical procedures were performed. Catalase immunoreactivity was evaluated in quail liver tissues. There was a statistically significant difference between the pollen group and the control group in terms of MDA levels. Strong catalase immunoreactivity was detected in the liver tissue of all groups. As a result, it is thought that the addition of pollen to the diet will contribute positively to the reduction of MDA levels.

Keywords

• Bee bread
• liver
• pollen
• quails

Kaynakça

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