Seryum Nitrat ve Trombositten Zengin Plazma Tedavilerinin Yanıklardan Sonra Staz Bölgesinin İyileşmesinde Sıçan Testis Dokusu Üzerindeki Etkilerinin Araştırılması

Öz

Yanık sonrası travma günlük yaşamda sıklıkla görülmektedir. Yanık yaralanması ciltte travmatik hasara neden olmakla birlikte, vücutta sistemik inflamatuar reaksiyonlar sonucu testis dokusu da dahil olmak üzere birçok doku ve organda da hasara neden olabilir. Seryum nitrat (SN) ve Trombositten Zengin Plazma (TZP), antiinflamatuar, antioksidan ve antiapoptotik özelliklere sahip doğal bileşiklerdir. Çalışmanın birinci gününde sıçanların cilt dokularında termal hasar oluşturuldu. Daha sonra CN grubundaki sıçanlar 0,04 M SN'li 30 dakikalık banyoda tutuldu. PRP grubundaki sıçanlara yara bölgesine 0,1 ml PRP intradermal enjeksiyonu yapıldı. Yanık sonrası yapılan spermatolojik incelemelerde anormal şekilli sperm sayısının arttığı ve sperm zarının bütünlüğünün bozulduğu görüldü. Histopatolojik incelemelerde testis dokularında tubüler duvarlarda incelme, spermatosit sayısında azalma ve spermatositlerde ileri derecede dejenerasyon ve nekroz gözlendi. Yanığın testis dokularında IL-1β, TNF-α ve iNOS düzeylerini artırarak inflamasyonu tetiklediği, 8-OHdG düzeylerini artırarak DNA hasarına neden olduğu ve ayrıca testis hücrelerinde Kaspaz 3 ekspresyonunun artmasına bağlı olarak apoptoza neden olduğu gözlendi. CN ve PRP tedavilerinin yanık sonrası anormal şekilli sperm sayısını azalttığı, spermlerin membran bütünlüğünü koruduğu, testis dokularında inflamasyonu, oksidatif stresi ve apoptozu baskıladığı belirlenmiştir.

Anahtar Kelimeler

• seryum nitrat
• trombositten zengin plazma
• Apoptozis
• inflamasyon
• yanık

Investigation of the Effects of Cerium Nitrate and Platelet-Rich Plasma Treatments on Rat Testicular Tissue in the Recovery of the Stasis Zone

Abstract

Post-burn trauma is common in daily life. Although a burn injury causes traumatic damage to the skin, it can also cause damage to many tissues and organs, including testicular tissue, as a result of systemic inflammatory reactions in the body. Cerium nitrate (CN) and Platelet-Rich-Plasma (PRP) are natural compounds having anti-inflammatory, anti-oxidant and anti-apoptotic properties. In this study, we aimed to investigate the efficacy of CN and PRP against the damage to testicular tissues after burns. Thermal damage was induced in the skin tissues of the rats on the first day of the study. Then, the rats in the CN group were kept in a 30-min bath of 0.04 M CN. The rats in the PRP group received 0.1 ml of PRP intradermal injections in the wound area. Spermatological examinations performed after burn revealed that abnormally shaped sperm counts increased and the integrity of the sperm membrane was impaired. In histopathological examinations, thinning of the tubular walls in testicular tissues was observed, as well as a decrease in spermatocyte numbers and severe degeneration and necrosis in the spermatocytes. It was also observed that the burn triggered inflammation in testicular tissues by increasing IL-1β, TNF-α and iNOS levels, caused DNA damage by increasing 8-OHdG levels, and furthermore caused apoptosis due to increased Caspase 3 expression in the testicular cells. It was determined that CN and PRP treatments reduced the number of abnormally shaped sperms after burn, maintained membrane integrity of sperms, and suppressed inflammation, oxidative stress and apoptosis in testicular tissues.

Kaynakça

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