Biosensor Platforms for Cancer Derived Exosomes Detection

Kübra Kelleci; Sevil Özer

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Kanser Kaynaklı Eksozom Tespitinde Biyosensör Platformlar

Öz

Erken teşhis kansere karşı mücadeledeki en büyük zorluklardan biridir. Geleneksel kanser tanı yöntemlerinin uzmanlık gerektirmesi, invaziv işlem içermesi, radyasyon maruziyeti, yüksek maliyet ve zaman kaybına neden olması gibi birtakım dezavantajlara sahiptir. Bu nedenle mevcut araştırmalar daha hızlı, güvenilir ve uygun maliyetli tanı yöntemlerinin geliştirilmesine odaklanmıştır. Vücut sıvılarında var olan biyobelirteçlerin kullanımı bu anlamda umut verici olmuştur. Hücreler tarafından vücut sıvılarına salgılanan ve hücreler arası iletişimden sorumlu olan ekzosomlar, doğal yolaklarla üretilen biyolojik nanopartiküllerdir. Tümör gelişimi esnasında mikroçevre koşullarında meydana gelen değişimin ekzosom sekresyonunu değiştirdiği yapılan çalışmalarca ortaya konmuştur. Tümör hücreleri yüksek hücresel aktiviteleri nedeniyle, sağlıklı hücrelere göre çok daha yüksek oranda ekzosom üretimi gerçekleştirmektedir. Dolayısıyla vücut sıvılarında bulunan ekzosomların sayısı, diğer hücrelere göre ciddi oranda zenginleştiği ve tek başına tanısal biyobelirteç olarak görev yapabildiği bilinmektedir. Kanser kaynaklı eksozomlar, kanserin erken teşhisi ve terapötik yanıt değerlendirmesi için oldukça ilgi çekicidir. Biyosensör, biyolojik bir bileşeni fizikokimyasal bir detektörle birleştiren bir analitin saptanması için kullanılan analitik bir cihazdır. Günümüzde, üstün analitik performans ve gerçek zamanlı ölçüm gösterdikleri için kanser biyosensörlerini geliştirmesine yönelik artan bir ilgi vardır. Ekzosom tespiti için biyosensör uygulamaların geliştirilmesi ve yaygınlaştırılması, teşhis cihazlarına ve kaynaklarına erişimi olmayan bölgelerdeki bireyler için oldukça avantaj sağlayacaktır. Geleneksel yöntemlerin dezavantajları göz önünde bulundurulduğunda biyosensör çalışmalarının daha ucuz, çoklu analit desteği sağlaması, yüksek hassasiyet sağlaması ve hızlı, güvenilir sonuçlar elde etmesi nedeniyle çok daha avantajlı olduğu söyleyebiliriz. Bu çalışmada farklı kanser hücrelerinden salgılanan eksozomların tespiti için biyosensör sistemlerdeki son gelişmeler özetlenmiştir. Ayrıca, bakım noktası testleri (POC) için kanserden salgılanan eksozomların kullanımı ve bu alandaki zorluklar sunulmaktadır.

Anahtar Kelimeler

Biyosensör, Biyobelirteç, Ekzosom, Tanı yöntem

Biosensor Platforms for Cancer Derived Exosomes Detection

Öz

Early diagnosis is one of the biggest challenges in the fight against cancer. Traditional cancer diagnosis methods have some disadvantages such as requiring expertise, involving invasive procedures, radiation exposure, high cost and loss of time. For this reason, current research has focused on the development of faster, reliable and cost-effective diagnostic methods. The use of biomarkers present in body fluids has been promising in this regard. Exosomes secreted by cells into body fluids and responsible for intercellular communication are biological nanoparticles produced by natural pathways. Studies have shown that the change in microenvironmental conditions during tumor development changes exosome secretion. Due to their high cellular activities, tumor cells produce a much higher rate of exosome than healthy cells. Therefore, it is known that the number of exosomes in body fluids is significantly enriched compared to other cells and can act as a diagnostic biomarker alone. Cancer-derived exosomes are very interesting for early detection of cancer and evaluation of therapeutic response. Biosensor is an analytical device used to detect an analyte that combines a biological component with a physicochemical detector. Nowadaysthere is an increasing interest in developing cancer biosensors with superior analytical performance and real-time measurement. The development and dissemination of biosensor applications for exosome detection will be very advantageous for individuals in regions where there is no access to diagnostic devices and resources. Considering the disadvantages of traditional methods, we can say that biosensor studies are much more advantageous because they are cheaper, provide multi-analyte support, provide high sensitivity, and obtain fast and reliable results. In this study, recent developments in biosensor systems for the detection of exosomes secreted from different cancer cells are summarized. In addition, the use of cancer-secreted exosomes for point of care testing (POC) and the challenges in this area are presented and discussed.

Anahtar Kelimeler

Biosensor, Biomarker, Exosome, Diagnostic method

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Birinci Basamak Sağlık Hizmetleri

Bölüm

Derleme

Yazarlar

Kübra Kelleci ^* Bu kişi benim
0000-0002-9409-2254
Türkiye

Sevil Özer Bu kişi benim
0000-0002-0186-763X
Türkiye

Yayımlanma Tarihi

30 Temmuz 2021

Gönderilme Tarihi

14 Haziran 2021

Kabul Tarihi

9 Temmuz 2021

Yayımlandığı Sayı

Yıl 2021 Cilt: 2 Sayı: 3

IZ

https://izlik.org/JA58DS45GB

APA

Kelleci, K., & Özer, S. (2021). Biosensor Platforms for Cancer Derived Exosomes Detection. Yeni Yüzyıl Journal of Medical Sciences, 2(3), 22-31. https://izlik.org/JA58DS45GB

AMA

1.Kelleci K, Özer S. Biosensor Platforms for Cancer Derived Exosomes Detection. JMS. 2021;2(3):22-31. https://izlik.org/JA58DS45GB

Chicago

Kelleci, Kübra, ve Sevil Özer. 2021. “Biosensor Platforms for Cancer Derived Exosomes Detection”. Yeni Yüzyıl Journal of Medical Sciences 2 (3): 22-31. https://izlik.org/JA58DS45GB.

EndNote

Kelleci K, Özer S (01 Temmuz 2021) Biosensor Platforms for Cancer Derived Exosomes Detection. Yeni Yüzyıl Journal of Medical Sciences 2 3 22–31.

IEEE

[1]K. Kelleci ve S. Özer, “Biosensor Platforms for Cancer Derived Exosomes Detection”, JMS, c. 2, sy 3, ss. 22–31, Tem. 2021, [çevrimiçi]. Erişim adresi: https://izlik.org/JA58DS45GB

ISNAD

Kelleci, Kübra - Özer, Sevil. “Biosensor Platforms for Cancer Derived Exosomes Detection”. Yeni Yüzyıl Journal of Medical Sciences 2/3 (01 Temmuz 2021): 22-31. https://izlik.org/JA58DS45GB.

JAMA

1.Kelleci K, Özer S. Biosensor Platforms for Cancer Derived Exosomes Detection. JMS. 2021;2:22–31.

MLA

Kelleci, Kübra, ve Sevil Özer. “Biosensor Platforms for Cancer Derived Exosomes Detection”. Yeni Yüzyıl Journal of Medical Sciences, c. 2, sy 3, Temmuz 2021, ss. 22-31, https://izlik.org/JA58DS45GB.

Vancouver

1.Kübra Kelleci, Sevil Özer. Biosensor Platforms for Cancer Derived Exosomes Detection. JMS [Internet]. 01 Temmuz 2021;2(3):22-31. Erişim adresi: https://izlik.org/JA58DS45GB

Kanser Kaynaklı Eksozom Tespitinde Biyosensör Platformlar

Öz

Anahtar Kelimeler

Biosensor Platforms for Cancer Derived Exosomes Detection

Öz

Anahtar Kelimeler

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

IZ

Kaynak Göster